Abstract: Thirty-week-old healthy Hy-Line Brown laying hens in two henhouses were chosen to evaluate the effects of complex Bacillus on fecal microbial flora and egg quality of laying hens. Hens in the control group were fed a basal diet, and hens in the experimental group were fed basal diet supplemented with complex Bacillus. The results showed as follows： compared with the control group, at 30 d, the amount of “Salmonella+Shigella” decreased significantly （P<0.05）, and Bacillus increased significantly （P<0.05） in the experimental group; at 60 d, the amount of total airborne bacteria and Escherichia coli decreased significantly （P<0.05）, bacillus increased significantly （P<0.05）, and the ratio of egg yolk increased significantly （P<0.05）; at 90 d, the amount of total airborne bacteria, Escherichia coli and “Salmonella+Shigella” decreased significantly （P<0.05）, and egg yolk color increased significantly （P<0.05）. The results indicated that dietary supplemented with complex Bacillus could significantly decrease the amounts of harmful microorganism to improve the intestinal healthy, and significantly increase the egg yolk ratio and color, and then improve the egg quality.

Key words: complex Bacillus, laying hens, fecal microbial flora, egg quality