Screening of lignocellulosic degradation complex strains

doi:10.14088/j.cnki.issn0439-8114.2021.16.009

Abstract

Abstract: In order to improve the degradation rate of biomass straw and screen high-efficiency biomass cellulose degrading bacteria, the corn stalk-rich rot was used as a bacterial source, and the cellulose-congo red selective medium was used for preliminary screening to separate high-efficiency cellulose-degrading bacteria. The initial screening was determined by the diameter of the transparent ring on the Congo red medium and the disintegration ability of the filter paper strip; The rescreening was carried out by the determination of the enzyme activity, including the determination of the activity of the filter paper strip and the determination of the activity of the carboxymethylcellulose enzyme. Screening step by step to form a complex strain of high-efficiency strains, the results showed that a group of corn stalk degradation complex strains were screened from the decayed corn stalks and in the enzyme activity assay, the C₁ strain had the highest filter paper activity of 8.52 U/mL; The N₁strain had the highest cellulase activity of 6.44 U/mL. By sequencing and identifying the strains of the selected strains, the dominant strains of composite bacteria were Trametes, Sarocladium, Streptomyces, Actinomycetales unclassified, Propionibacterium.

Key words: compound bacteria, lignocellulose, screening, flora, degradation

CLC Number:

Q939

SUN Miao-miao, LI Juan, LIU Guo-qing, ZHOU Chun-yang, WEI Zhang-qi, WU Jian-xin. Screening of lignocellulosic degradation complex strains[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(16): 44-49.

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