Screening of high-efficiency bioflocculant-producing actinobacteria and preliminary optimization of medium composition

doi:10.14088/j.cnki.issn0439-8114.2021.24.022

Abstract

Abstract: In order to develop a strain with high flocculation activity for rural sewage treatment, actinomycete ws75382 with the flocculation rate 91.9% of kaolin was identified by 16S rDNA, and its medium composition were optimized by single factor test and orthogonal test. 16S rDNA identification showed that actinomycetes ws75382 had the highest similarity with Streptomyces cyslabdanicus. The initial culture medium of actinomycetes ws75382 had many components, and the concentration of carbon and nitrogen sources was higher. The optimized medium composition reduced the nitrogen sources by one component, and the concentrations of carbon and nitrogen sources reduced 40% and 30%, respectively. The optimized culture medium components were soybean powder 15 g/L, glucose 14 g/L, sodium chloride 8 g/L and calcium carbonate 3 g/L, and under the conditions the flocculation rate of kaolin was nealy 100%.

Key words: rural sewage treatment, actinomycetes, flocculation, strain identification, medium composition

CLC Number:

X703

TIAN Yu-xi, CHEN Ling, MIN Yong, YANG Zi-wen. Screening of high-efficiency bioflocculant-producing actinobacteria and preliminary optimization of medium composition[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(24): 94-97.

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