Study on treatment of nickel-containing wastewater by alga-bacteria biofilm and changes of the extracellular polymeric substances

doi:10.14088/j.cnki.issn0439-8114.2023.03.022

Abstract

Abstract: To explore the feasibility of algal-bacteria biofilm's treatment for nickel-containing wastewater, alga-bacteria biofilm was obtained by filming algae and bacteria enrichment in natural urban water, and nickel-containing solution was treated under different operating parameters to study the adsorption amount of nickel and the change of extracellular polymer substance（EPS） content of algal bacteria biofilm under different conditions. The results showed that the algas after enrichment and filming belonged to Chlorella, which had a higher treatment effect on nickel-containing wastewater. The treatment effect was best when pH was 8 and the temperature was 35 ℃. Nickel adsorption equilibrium was reached within 3 days and the removal rate was more than 70%. At the same time, the content of polysaccharides in alga-bacteria biofilm with time was positively correlated with nickel adsorption amount, which proved that it was the main substance in nickel adsorption, while ATP content could reflect the stress process of alga-bacteria biofilm. Under the stress of nickel, the content of extracellular polymer substances in alga-bacteria biofilm decreased with the increase of stress. From the perspective of adsorption amount, its tolerance to nickel was 10 mg/L. Within this range, adsorption amount increased with the increase of the nickel concentration, up to 2.358 mg. However, adsorption amount decreased significantly when the nickel concentration continued to increase.

Key words: algae biofilm, extracellular polymer substance （EPS）, nickel （Ni）, adsorption amount, removal rate

CLC Number:

X703

REN Yun-yun, ZHANG Sha, PEI Cuo-ping, ZHANG Zhi-lin. Study on treatment of nickel-containing wastewater by alga-bacteria biofilm and changes of the extracellular polymeric substances[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(3): 135-140.

References

[1] 周建军,周桔,冯仁国.我国土壤重金属污染现状及治理战略[J].中国科学院院刊,2014,29(3): 315-320,350,272.
[2] 王丙烁,黄益宗,王农,等.镍污染土壤修复技术研究进展[J].农业环境科学学报,2018,37(11):2392-2402.
[3] 陈远. 遵义钼镍矿下游水体污染及生物毒性研究[J].科技创新与应用,2012(23):121-122.
[4] SALT D E,BLAYLOCK M,KUMAR N P,et al.Phytoremediation: A novel strategy for the removal of toxic metals from the environment using plants[J].Biotechnology,1995,13(5):468-474.
[5] 张敏,郜春花,李建华,等.重金属污染土壤生物修复技术研究现状及发展方向[J].山西农业科学,2017, 45(4):674-676.
[6] 凌娜,刘小瑞,李玮璐,等.应用藻类进行重金属污染水体生物修复的研究进展[J].水产学杂志,2022,35(1):89-96.
[7] 杨荧,刘莉文,李建宏.微藻富集重金属的机制及在环境修复中的应用综述[J].江苏农业科学,2019,47(21):88-94.
[8] 范彩彩. 鼠尾藻对水体重金属铅、铜、锌、镉的生物吸附效应研究[D].浙江舟山:浙江海洋学院,2013.
[9] MATA Y N,BLÁZQUEZ M L,BALLESTER A,et al. Characterization of the biosorption of cadmium, lead and copper with the brown alga Fucus vesiculosus[J].J Hazard Mater,2008,158(2-3):316-323.
[10] 马艳,宋文娟,赵晨曦.藻菌生物膜胞外聚合物与铅锌尾矿重金属的络合配位作用[J].环境科学研究, 2018,31(7):1296-1302.
[11] TIWARI S,DIXIT S,VERMA N.An effective means of biofiltration of heavy metal contaminated water bodies using aquatic weed Eichhornia crassipes[J].Environmental monitoring & assessment,2007,129(1-3):253-256.
[12] DIXIT S,DHOTE S.Evaluation of uptake rate of heavy metals by Eichhornia crassipes and Hydrilla verticillata[J].Environmental monitoring & assessment,2010,169(1-4):367-374.
[13] 肖明,王雨净.微生物实验[M].北京:科学出版社,2008.
[14] 曹轶姝. 原子吸收光度法连续测定矿石中的铜铅锌钴镍[J].四川建材,2012,38(2):105-106.
[15] 日本微生物研究法讨论会.微生物学实验法[M].程光胜译.北京:科学出版社,1981.
[16] 邱廷省,唐海峰.生物吸附法处理重金属废水的研究现状及发展[J].南方冶金学院学报,2003(4):65-69.
[17] 高敏,李茹.菌藻共生生物膜对重金属镉的去除[J].西安工程大学学报,2016,30(2):170-176.
[18] 季秀玲,魏云林.低温微生物环境污染修复技术研究进展[J].环境污染治理技术与设备,2006,7(10):6-11.
[19] 李楠,王秀蘅,亢涵,等.pH对低温除磷微生物种群与聚磷菌代谢的影响[J].环境科学与技术,2013, 36(3):9-11,19.
[20] 江娜,宁增平,郭圆,等 .环境pH对微生物生物膜吸附重金属的影响研究进展[J].地球与环境,2021, 49(2):216-226.
[21] 陈燕飞. pH对微生物的影响[J].太原师范学院学报(自然科学版),2009,8(3):121-124,131.
[22] 吴静,陈书涛,胡正华,等.不同温度下的土壤微生物呼吸及其与水溶性有机碳和转化酶的关系[J].环境科学,2015,36(4):1497-1506.
[23] 黄飞. 蜡状芽孢杆菌对水体中镉的吸附特性与机理研究[D].广州:华南理工大学,2013.
[24] 李韵诗,冯冲凌,吴晓芙,等.重金属污染土壤植物修复中的微生物功能研究进展[J].生态学报,2015,35(20): 6881-6890.
[25] 邓闻杨,罗学刚,罗蓝,等.三种微生物对铀胁迫下凤眼莲荧光生理及铀累积特性的影响[J].农业环境科学学报,2018, 37(8):1626-1633.
[26] 张帆,李菁,谭建华,等.吸附法处理重金属废水的研究进展[J].化工进展,2013,32(11):2749-2756.
[27] 姚雪丹,付建红,徐彤,等.重金属胁迫下的微生物代谢组学研究进展[J].生物资源,2020,42(6):678-685.
[28] 杨东方,曹海荣,高振会,等.胶州湾水体重金属HgⅠ.分布和迁移[J].海洋环境科学,2008(1):37-39.