Abstract: Taking the opportunity of the “Third National Crop Germplasm Resources Census and Collection Action” of the Ministry of Agriculture and Rural Affairs, the investigation, collection, evaluation and identification of Astragalus chinensis L. germplasm resources in Guangxi were carried out. The results showed that the 58 collected local resources were mainly distributed in the middle and low altitude areas of 0~400 meters in northeastern Guangxi. Phenotypic analysis of 12 agronomic traits showed rich genetic diversity. Principal component analysis divided 12 agronomic traits into 3 principal components, namely seed yield, biological yield, and growth period characteristics, with a cumulative contribution rate of 72.089%. By weighted average calculation, the top 10 germplasm resources in the comprehensive ranking were GXLF20180011, GXLF2019109, GXLF2019156, GXLF2019118, GXLF2019112, GXLF2019117, GXLF20180006, GXLF2019125, GXLF2019111, and GXLF2019136. The total growth period of the top 10 germplasm resources was 134~147 d, with stem diameter of 2.83~3.66 mm during the peak flowering period, branch length of 41.63~54.73 cm during the peak flowering period, branch number per plant of 2.83~4.19, flower number per branch of 5.85~6.99, total flower number of 17.55~26.23 per plant, pod bearing flower number of 14.33~19.97 per plant, and fresh grass yield of 10 458.15~48 792.75 kg/hm², hay yield of 1 037.36~5 724.34 kg/hm², and seed yield of 246.75~991.20 kg/hm².

Key words: Astragalus sinicus L., germplasm resources, collection, phenotypic diversity, Guangxi