Physiological mechanisms of exogenous gibberellin treatment affecting the seed germination process of Fraxinus hupehensis Chiú. Shang et Su

doi:10.14088/j.cnki.issn0439-8114.2024.05.013

Abstract

Abstract: The seeds of Fraxinus hupehensis stored at normal temperature for 2 years were taken as the research object. Before the experiment, the radicle end covering tissue was removed to expose the radicle. Taking water as a control, after soaking the seeds with 150 mg/L gibberellin solution for 24 h, the physiological and biochemical indexes and the contents of endogenous hormones in seeds during germination were measured. The results showed that gibberellin treatment could enhance seed germination rate, with a germination rate of 24.67% after gibberellin treatment compared to 16.67% in the control group. During germination, gibberellin-treated seeds exhibited a trend of initially increasing and then decreasing in soluble sugar content. Soluble protein content showed a decreasing trend but remained significantly higher than the control group at all stages. Superoxide dismutase （SOD）, peroxidase （POD）, and catalase （CAT） activities showed an increasing trend on the whole. The content of endogenous jasmonic acid methyl ester （JA-Me） in seeds after gibberellin treatment was significantly lower than that in the control, which favored an increase in endogenous gibberellin （GA₃） and zeatin riboside （ZR） content. The ratios of gibberellin to abscisic acid （GA₃/ABA） and zeatin riboside to abscisic acid （ZR/ABA） were significantly positively correlated with the ratio of indole-3-acetic acid to abscisic acid （IAA/ABA） at 0.01 and 0.001 levels, respectively, and GA₃/ABA was significantly positively correlated with ZR/ABA at 0.001 level. Gibberellin immersion significantly increased the germination rate of Fraxinus hupehensis seeds, and the seeds treated with gibberellin exhibited a stronger balance of protective enzyme activity system, delayed membrane damage, and the resistance was better. The lower levels of endogenous JA-Me and the higher levels of endogenous GA₃ and ZR favored the initiation of seed germination and the release of dormancy.

Key words: Fraxinus hupehensis Chiú. Shang et Su;, seed, physiology and biochemistry, germination, gibberellin

CLC Number:

S792.41

ZHAO Yi-ning, LIU Yan-hong, ZHENG Peng-li, ZHOU Ming-qin. Physiological mechanisms of exogenous gibberellin treatment affecting the seed germination process of Fraxinus hupehensis Chiú. Shang et Su[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 70-76.

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