Content determination and subcellular localization of ester catechins of small and medium-leaf teas

doi:10.14088/j.cnki.issn0439-8114.2024.05.017

HUBEI AGRICULTURAL SCIENCES ›› 2024, Vol. 63 ›› Issue (5): 98-100.doi: 10.14088/j.cnki.issn0439-8114.2024.05.017

• Horticulture & Local Products • Previous Articles Next Articles

Content determination and subcellular localization of ester catechins of small and medium-leaf teas

MAN Jia-xu^a,b, GAO Zi-qi^a,b, WU Si-min^a,b, WANG Dong-xue^a,b, ZHAO Xue-ting^a,b, ZHANG Dong-ying^b

a.College of Food Science and Technology; b. Key Laboratory of Pu’er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650000, China

Received:2022-11-01 Online:2024-05-25 Published:2024-06-04

Abstract

Abstract: In order to clarify the subcellular spatial localisation of ester catechins in small and medium-leaf teas, five small- and medium-leaf tea samples of Shilixiang, Guilv No.1, Zhenong 113, Fuding Dabai Tea and Zhenong 139 were taken as the test materials, and the content of ester catechins in the five tea samples was detected using high-performance liquid chromatography （HPLC）, and the vesicles in the cells of the samples were localised using neutral red staining. Subcellular localisation of ester catechins in tea samples was performed by immunohistochemistry. The results showed that all five types of small and medium-leaf teas contained different levels of ester catechins, with the highest content in Shilixiang tea; under neutral red staining, the vesicles in Shilixiang tea samples were round or oval; under immunohistochemistry staining, the ester catechins of the five types of small and medium-leaf teas were located in the vesicles at the subcellular level.

Key words: small and medium-leaf teas, ester catechins, immunohistochemistry, subcellular localisation, determination of content

CLC Number:

Q946.84⁺1

MAN Jia-xu, GAO Zi-qi, WU Si-min, WANG Dong-xue, ZHAO Xue-ting, ZHANG Dong-ying. Content determination and subcellular localization of ester catechins of small and medium-leaf teas[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 98-100.

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Content determination and subcellular localization of ester catechins of small and medium-leaf teas

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