Effects of Bacillus mojavensis ZLX10 on the neutral aroma components of fermented cigar tobacco leaves

doi:10.14088/j.cnki.issn0439-8114.2024.06.028

Abstract

Abstract: To investigate the effect of mono-bacterial fermentation of tobacco-derived microorganisms on volatile aroma components of cigar tobacco leaves, the neutral aroma components of cigar tobacco leaves fermented by Bacillus mojavensis ZLX10 were detected by GC-MS, the differential neutral aroma components were screened, and the correlation analysis was done with the sensory scores. Combined with the change of ROAV value, the key aroma components to improve the sensory quality of cigar tobacco leaves by ZLX10 were identified. The results showed that after ZLX10 fermented cigar tobacco leaves, the total aroma and the contents of most neutral aroma components obviously increased. A total of 15 differential neutral aroma components were screened, and a total of 10 aroma components were significantly increased （P<0.05）. Among them, megastigmatrienone A, megastigmatrienone B, 6-methyl-5-hepten-2-one, 2,6,6-trimethyl-2-cyclohexene-1,4-dione, solanone and phytoalcohol were significantly positively correlated with the aroma of cigar tobacco leaves （P<0.05）, which could be considered as the key differential aroma components to increase the dry aroma and floral aroma of cigar tobacco leaves. Five aroma components significantly decreased （P<0.05）, among which phenylacetaldehyde and 2,4-di-tert-butyl-6-nitrophenol were significantly positively correlated with cigar irritability （P<0.05）, and had a greater negative impact on the aroma of tobacco leaves. It could be seen that ZLX10 fermentation could promote the quality and quantity of cigar tobacco leaves aroma, which provided a basis for the fermentation of cigar tobacco leaves by artificial inoculation.

Key words: cigar tobacco leaves, Bacillus mojavensis, tobacco fermentation, neutral aroma components

CLC Number:

S572

ZHAO Xiao-chao, LI Lin-lin, SHI You-zhi, ZHANG Bei-bei, ZHOU Ya-bin, YU Yan, ZHANG Jing-ya, QU Huan, PU Qian, HUANG You-yi, PAN Yong. Effects of Bacillus mojavensis ZLX10 on the neutral aroma components of fermented cigar tobacco leaves[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(6): 173-180.

References

[1] 牛浩,周中宇,白金莹,等.雪茄烟发酵的研究进展[J].湖南文理学院学报(自然科学版), 2020,32(4):60-63,68.
[2] SRIVASTAVA R P.Biochemical transformations in leaf pigments and chemical constituents during air-curing of cigar wrapping tobacco[J].Tobacco research,1988,14(2):83-88.
[3] 张玉姣,宋金鹏,郭学民,等.烟叶微生物菌群变化及微生物在烟叶中的应用现状[J].中国酿造,2022,41(6):31-36.
[4] 李宁,汪长国,曾代龙,等.蜡样芽孢杆菌(Bacillus cereus)筛选鉴定及在雪茄烟叶发酵中的应用研究[J].中国烟草学报,2012,18(2):65-69.
[5] 薛磊,郑泽浩,郭志刚,等.烟草增香细菌的筛选及其作用效果[J].中国烟草科学,2019,40(5):60-67.
[6] 罗家基,朱子高,罗毅,等.微生物在烟叶发酵过程中的作用[J].烟草科技,1998(1):6.
[7] 帅瑶,陶菡,田运霞,等.复合菌种发酵烟叶产酶及挥发性风味物质变化[J].河南农业科学,2020,49(10):162-175.
[8] 黄晓春. 降碱增香微生物制剂改善烟叶品质研究与应用[D].长沙:湖南农业大学,2010.
[9] 李勇,王猛,陈剑明,等.出芽短梗霉OF-01菌株的筛选及其在烟草香料中的应用[J].轻工学报,2017,32(4):49-57.
[10] 黄申,夏璠,钱玉梅,等.西柏三烯-4,6-二醇降解产香菌培养基条件优化[J].烟草科技,2017,50(12):55-60,69.
[11] ROBERTS M S,NAKAMURA L K,COHAN F M.Bacillus mojavensis sp.nov.,distinguishable from Bacillus subtilis by sexual isolation,divergence in DNA sequence,and differences in fatty acid composition[J].International journal of systematic bacteriology,1994,44(2):256-264.
[12] 刘龙,荣华,郑童童,等.莫海威芽孢杆菌对梨腐烂病的抑菌防病效果[J].中国农学通报,2022,38(18):140-146.
[13] 冯中红,畅涛,杨成德,等.一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillus mojavensis ZA1)培养条件的优化[J].草业学报,2016,25(2):77-86.
[14] 郭宏萍. 优良菌株组合发酵对食醋大曲和酿造过程中的代谢调控[D].山西晋中:山西农业大学,2021.
[15] 李晓梅. 山西老陈醋源优良芽孢杆菌的筛选、互作及原位强化研究[D].山西晋中:山西农业大学,2020.
[16] 孙建平. 山西老陈醋酿造川芎嗪的生成规律及菌株代谢调控[D].山西晋中:山西农业大学,2021.
[17] YC/T 31—1996,烟草及烟草制品试样的制备和水分的测定烘箱法[S].
[18] 赵晓丹,史宏志,钱华,等.不同类型烟草常规化学成分与中性致香物质含量分析[J].华北农学报,2012,27(3):234-238.
[19] 马京民,刘国顺,时向东,等.主成分分析和聚类分析在烟叶质量评价中的应用[J].烟草科技,2009(7):57-60.
[20] 里奥·范海默特.化合物嗅觉阈值汇编(第二版)[M]. 北京:科学出版社,2018.
[21] 刘登勇,周光宏,徐幸莲.确定食品关键风味化合物的一种新方法:“ROAV”法[J].食品科学,2008(7):370-374.
[22] 陶陶,来苗,姬小明,等.烤烟类胡萝卜素及其降解产物与香气特性的关系研究[J].西南农业学报,2016,29(2):294-297.
[23] 王芳,欧明毅,杨超,等.茅台酒致香微生物对烟叶致香成分影响[J].贵州农业科学,2018,46(6):89-93,97.
[24] 毛多斌,黄晓玉,周利峰,等.枯草芽孢杆菌分离鉴定及其对烟叶化学成分和吸味品质的影响[J].烟草科技,2022,55(8):10-19.
[25] 薛云,宁振兴,苏赞,等.基于芽孢杆菌固态发酵技术提高广西河池C4F烟叶品质的研究[J].轻工学报,2022,37(5):76-84.
[26] 毛多斌,贾春晓,张峻松,等.茄酮及其降解产物系列香料合成研究进展[J].郑州轻工业学院学报,1998(2):59-63.
[27] 孔宁川,陈永宽,杨伟祖,等.异茄酮——5-异丙基-8-甲基-5,8-壬二烯-2-酮的合成及在烟草加香中的应用[J].云南化工,2002(3):4-6.
[28] 赵铭钦,刘金霞,黄永成,等.烟草质体色素与烟叶品质的关系综述[J].中国农学通报,2007(7):135-138.
[29] 刘哲. 基于香气活力值的烟叶、卷烟、烟草添加剂主要香气成分研究[D].昆明:昆明理工大学,2020.
[30] 谢剑平. 烟草香原料[M].北京:化学工业出版社,2009.
[31] 于建军,杨寒文,毕庆文,等.烤烟中性致香成分与香气质量的典型相关分析[J].甘肃农业大学学报,2009,44(2):72-76,104.
[32] 王能如,李章海,王东胜,等.我国烤烟主体香味成分研究初报[J].中国烟草科学,2009,30(3):1-6.
[33] 刘金,刘复初,朱洪友.巨豆三烯酮的合成研究概况[J].云南化工,2006(1):38-40.
[34] 于航,刘砚婷,尚梦琦,等.基于致香成分分析的雪茄烟产地间差异[J].烟草科技,2021,54(9):58-71.
[35] 貊志杰,邓帅军,史素娟,等.烤烟品种中烟特香301烤后烟叶石油醚提取物分析[J].中国烟草科学,2022,43(3):71-77.
[36] 嵇伟彬,刘盼盼,许勇泉,等.几种乌龙茶香气成分比较研究[J].茶叶科学,2016,36(5):523-530.
[37] 田永峰,杨柳,董高峰,等.解淀粉芽孢杆菌GUHP-86在口含烟烟草原料发酵中的提质降害研究[J].中国酿造,2019,38(10):133-137.
[38] 周富臣,戚万敏,张红双,等.氧化异佛尔酮及其在卷烟加香中的作用[J].烟草科技,1997(1):27.
[39] 于建军,庞天河,章新军,等.鄂西南烤烟吸食质量与致香物质的关系[J].华中农业大学学报,2006(4):355-358.
[40] 姜慧娟,赵铭钦,任伟,等.浓香型烤烟中性致香成分及多酚含量与香气质量的关系研究[J].中国烟草学报,2014,20(5):25-30.
[41] 许美玲,焦芳婵,吴兴富,等.烤烟种质资源致香成分分析[J].江西农业学报,2022,34(2):127-139.
[42] 李许涛,武广鹏,高相彬,等.平顶山烟区烤烟中性香气成分与感官评吸质量分析[J].安徽农业科学,2016,44(31):48-51,57.
[43] 周昆,周清明,胡晓兰.烤烟香气物质研究进展[J].中国烟草科学,2008(2):58-61.
[44] 王鹏泽,来苗,陶陶,等.不同香型烤烟主要香味物质成分与香韵指标的关系研究[J].中国农业科技导报,2015,17(3):126-135.
[45] 刘欢,李皓,陈长武,等.β-葡萄糖苷酶对‘双优’干红葡萄酒理化性质和香气物质的影响[J].食品与发酵工业,2018, 44(11):138-146.
[46] 谢春梅,焦红茹,曹芳玲.宁夏青铜峡小产区不同品种干红葡萄酒的香气成分分析[J].中国酿造,2018,37(1):170-176.
[47] 姚烨岑,郭明,叶博,等.新型水果时尚黄酒中风味物质的GC-MS检测分析及指纹图谱构建[J].酿酒科技,2022(4):127-131.