Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City

doi:10.14088/j.cnki.issn0439-8114.2024.10.010

Abstract

Abstract: The pathogenic fungi of the black spot of Brassica pekinensis in Shiyan City, Hubei Province were identified and analyzed, and the corresponding preventive measures were proposed. The pathogenic fungi were isolated from diseased leaves by tissue separation and identified by rDNA ITS and Alt-α 1 gene sequence alignment. The results showed that a new Alternaria sp. fungus was found, which was presumed to be the pathogenic fungus causing the local cabbage black spot disease in Shiyan City. In addition, it was confirmed that the joint analysis of ITS sequence and Alt-α 1 gene sequence could distinguish three common Alternaria fungi： Alternaria Brassicola, Alternaria japonica and Alternaria brassicae.

Key words: Chinese cabbage（Brassica pekinensis）, rDNA ITS, black leaf spot disease, pathogenic fungi, separation and identification, Shiyan City, Hubei Province

CLC Number:

S436.341

SHI Xian-ming, HE Ya-hui, HU Yan-ping, WU Fei-yang, PENG Xiao-xuan, ZHANG Yong-hong, YOU Lei, WEN Yi-hua. Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(10): 59-63.

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