Analysis of genetic diversities in Hubei local apricot varieties with simple sequence repeat（SSR） flourescence markers

doi:10.14088/j.cnki.issn0439-8114.2024.10.017

Abstract

Abstract: To explore the genetic diversity of local apricot variety resources in Hubei Province, 24 local apricot varieties materials were taken as test materials, and SSR molecular markers combined with fluorescence capillary electrophoresis technology were used for the study. The results showed that 14 pairs of primers from apricots, peaches, almonds, and cherries amplified a total of 162 polymorphic effective allele loci, with an average of 11.571 alleles per locus. The total number of effective alleles was 90.312, with an average of 6.451 effective alleles per locus. The average values of observed heterozygosity （Ho）, expected heterozygosity （He）, and Shannon's information index （I） were 0.652, 0.813, and 2.031, respectively. The average value of polymorphic information content （PIC） was 0.794, indicating that the primers for the selected related species had high universality and rich polymorphism. At a similarity coefficient of 0.580, 24 varieties were divided into three large groups. The first group was independently composed of Hongping apricot, the second group consisted of four varieties including Langmei and Xingmei et al, and the third group consisted of 19 varieties including rice apricot and Wuzu apricot. It could be seen that local apricot varieties in Hubei Province had rich genetic diversity, and SSR specific primers could effectively identify Hubei local apricot varieties.

Key words: local apricot, germplasm resources, fluorescent labeled SSR marker, genetic diversity, Hubei Province

CLC Number:

S662.2

YE Yuan-ming, CAI Hua, TIAN Rui, DONG Zhi-dan, ZHANG Yu, JIANG Ying-chun, WU Li-ming, HE Li-gang, LIU Mo-fa. Analysis of genetic diversities in Hubei local apricot varieties with simple sequence repeat（SSR） flourescence markers[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(10): 97-101.

References

[1] HEDIA B,IVAN S,CHRISTOPHER S, et al.Genetic structure of a worldwide germplasm collection of Prunus armeniaca L. reveals three major diffusion routes for varieties coming from the species’ center of origin[J]. Frontiers in plant science, 2020,11: 1-17.
[2] 张加延. 全国李与杏资源考察报告[J]. 中国果树,1990(4):29-34.
[3] HAGEN L S, CHAIB J, FADY B, et al.Genomic and cDNA microsatellites from apricot (Prunus armeniaca L.)[J]. Molecular ecology notes, 2004, 4: 742-745.
[4] DIETHARD T.Hypervariabflity of simple sequences as a general source for polymorphic DNA markers[J]. Nucleic acids research, 1989, 17(16): 6463-6471.
[5] 刘志琨,陈雪峰,王端,等. 基于SSR标记的普通杏种质资源遗传多样性分析[J/OL]. 分子植物育种, 1-22[2023-07-24].
[6] 董胜君,温佳星,卢彩云,等. 野杏经济性状SSR关联分析及优异等位变异挖掘[J]. 经济林研究, 2022, 40(2): 71-82.
[7] 赵海娟,章秋平,马小雪,等. 杏高通量荧光SSR检测体系及分子身份证的构建[J]. 分子植物育种, 2023, 21(24): 8134-8143.
[8] 斯钦毕力格,包文泉. 用于杏种质资源遗传多样性分析的SSR引物的筛选[J]. 分子植物育种, 2019, 17(5): 1551-1557.
[9] 董胜君,王新鑫,张皓凯,等. 基于SSR标记的东北杏遗传多样性分析及指纹图谱构建[J]. 沈阳农业大学学报,2021,52(6): 668-676.
[10] FORSTER B P,RUSSELL J R, ELLIS R P.Locating genotypes and genes for abiotic stress tolerance in barley: A strategy using maps, markers and the wild species[J]. New Phytol,1997,137:141-147.
[11] 何天明,陈学森,高疆生,等.新疆栽培杏群体遗传结构的 SSR分析[J].园艺学报,2006,33(4):809-812.
[12] 张淑青,刘冬成,刘威生,等.普通杏品种SSR遗传多样性分析[J].园艺学报,2010,37(1):23-30.
[13] 王玉安,欧巧明,陈建军,等.甘肃地方杏品种资源的SSR遗传多样性分析[J].西北农业学报,2013,22(3):98-100.
[14] 赵海娟,章秋平,马小雪,等. 杏高通量荧光SSR检测体系及分子身份证的构建[J]. 分子植物育种,2023,21(24): 8134-8143.
[15] 苑克俊,牛庆霖,葛福荣,等. 利用荧光SSR标记构建杏新品系的分子身份证[J]. 北方园艺, 2018(4): 34-40.
[16] 王璐伟,沈志军,李贺欢,等.基于SSR荧光标记的79份桃种质遗传多样性分析[J].中国农业科学,2022,55(15):3002-3017.
[17] IEZZONI A, SCHMIDT H, ALBERTINI A.Cherries (Prunus)[M]. Intl. Soc. Hort. Sci.,Wageningen, The Netherlands,1990.
[18] CANTINI C,LEZZONI A F,LAMBOY W F, et al.DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats[J]. Journal of the Americal society for horticultural science, 2001,126(2):205-209.
[19] BAO W, WU T, LI T, et al.Genetic diversity and population structure of Prunus mira (Koehne) from the Tibet plateau in China and recommended conservation strategies[J]. PLoS ONE, 2017,12(11): e0188685.
[20] DIRLEWANGER E, COSSON P, TAVAUD M, et al.Development of microsatellite markers in peach[Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.)[J]. Theor Appl Genet, 2002, 105(1):127-138.
[21] CIPRIANI G,LOT G, HUANG W G, et al.AC/GT and AG/CT microsatellite repeats in peach (Prunus persica L. Batsch) isolation, characterization and cross-species amplification in Prunus[J]. Theor Appl Genet, 1999, 99(1-2):65-72.
[22] MASATOSHI N, FUMIO T, YOSHIO T.Accuracy of estimated phylogenetic trees from molecular data[J]. Journal of molecular evolution, 1983(19):153-170.