Population genetic structure analysis of camellia anthracis fungus in the main oil tea producing areas of China

doi:10.14088/j.cnki.issn0439-8114.2024.01.038

Abstract

Abstract: Using 168 strains from 16 geographical populations of camellia anthracis fungus （Colletotrichum camelliae Abel.）, a dominant species of camellia anthracis in the main camellia producing areas of China, as the test materials, ISSR molecular marker technology was used to analyze genetic diversity and population genetic structure. The percentage of polymorphic loci （PPB） for 16 geographical populations was 98.99%, the Nei’s gene diversity index （He） was 0.28, the Shannon information diversity index （Is） was 0.43, the average genetic similarity （I） was 0.834, and the average genetic distance （D） was 0.183. This indicated that the genetic diversity level and heterogeneity of camellia anthracis fungus were high, and there was a certain degree of genetic variation among populations. There was no correlation between genetic distance and geographical distance. The total genetic diversity （Ht）, genetic differentiation coefficient （Gst）, and gene flow （Nm） of the five provincial-level populations in Hubei, Zhejiang, Jiangxi, Hunan, and Guangxi provinces were 0.274 8, 0.517 4, and 0.466 4, respectively. This indicated that although the five provincial-level populations of camellia anthracis fungus were differentiated, there was no gene flow phenomenon （Nm<1）.

Key words: camellia（Camellia oleifera Abel.）, anthracis fungus（Colletotrichum camelliae）, ISSR, genetic diversity, population genetic structure, oil tea producing areas

CLC Number:

S763.1

WANG Yi-xun, XU Xiao-wen, ZHENG Lu, HUANG Jun-bin, XIE Xian-bin. Population genetic structure analysis of camellia anthracis fungus in the main oil tea producing areas of China[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(1): 212-217.

References

[1] 陈永忠,彭邵峰,王湘南,等. 油茶子代林群产量性状的研究[J]. 中南林学院学报,2006,26(5):8-14.
[2] 庄瑞林. 中国油茶[M]. 北京:中国林业出版社,2008.
[3] 田朝光. 普通油茶重要性状及脂肪酸组成遗传变异研究[D]. 北京:中国林业科学研究院,2000.
[4] 张联辉. 发展油茶产业实现生态富民[J]. 安徽科技,2009(8):22-23.
[5] 刘幼丽. 我国油茶文献研究分析[J]. 农业图书情报学报,2007,19(4):166-169.
[6] LI H, ZHOU G Y, LIU J A, et al.Population genetic analyses of the fungal pathogen Colletotrichum fructicula on tea-oil tree in China[J]. PLoS one, 2016, 11(6): e0156841.
[7] WANG Y X, CHEN J Y, XU X W, et al.Identification and characterization of Colletotrichum species associated with anthracnose disease of Camellia oleifera in China[J]. Plant disease, 2020, 104:474-482.
[8] 朱丹雪,周国英,徐建平,等. 果生刺盘孢菌Colletotrichum fructicola 群体遗传结构研究[J]. 菌物学报,2015, 34(3): 366-374.
[9] 李杨,李河,周国英,等. 油茶暹罗刺盘孢菌群体遗传结构分析[J]. 植物保护,2017,43(3):49-54.
[10] 方中达. 植病研究法(第三版)[M]. 北京:中国农业出版社,1998.
[11] SAMBROOK J, FRISCH E F, MANIATIS T.Molecular cloning: A laboratory manual (2^nd edition)[M]. New York: Cold spring harbor laboratory, 2001.
[12] RAMPERSAD S N.Genetic structure of Colletotrichum gloeosporioides sensu lato isolates infecting papaya inferred by multiocus ISSR markers[J]. Phytopathology, 2013, 103:183-189.
[13] HARTL D L, CLARK A G.Principles of population genetics[M].Sunderland,MA:Sinauer associates,2007. 565.
[14] FREEMAN S, KATAN T, SHABI E.Characterization of Colletotrichum gloeosporioides isolates from avocado and almond fruits with molecular and pathogenicity tests[J]. Applied environmental microbiology, 1996, 62(3): 1014-1020.
[15] DEAN R, VAN KAN J A L, PRETRIUS Z A, et al. The top 10 fungal pathogens in molecular plant pathology[J]. Molecular plant pathology, 2012, 13(7): 414-430.
[16] HYDE K D, CAI L, CANNON P F, et al.Colletotrichum-names in current use[J]. Fungal diversity, 2009, 39: 147-182.
[17] CHATTAOUI M, RAYA M C, BOURI M, et al.Characterization of a Colletotrichum population causing anthracnose disease on olive in northern Tunisia[J]. Journal of applied microbiology, 2016,120,1368-1381.
[18] 张小飞,李晓,崔丽娜,等. 我国玉米灰斑病菌遗传多样性的ISSR分析[J].植物保护学报,2015,42(6):908-913.
[19] 王子迎, 王源超, 张正光, 等. 中国和美国大豆疫霉群体遗传结构的ISSR分析[J]. 生物多样性, 2007,15(3):215-223.
[20] MAHMODI F, KADIR J B, PUTCH A, et al.Genetic diversity and differentiation of Colletotrichum spp. isolates associated with Leguminosae using multigene loci, RAPD and ISSR[J]. Plant pathology, 2014, 30(1):10-24.
[21] GRÜNIG C R, SIEBER T N, HOLDENRIEDER O. Characterisation of dark septate endophytic fungi(DSE) using inter-simple-sequence-repeat-anchored polymerase chain reaction(ISSR-PCR) amplification[J]. Mycological research, 2001, 105(1):24-32.
[22] BROWN A E,SREENIVAPRASAD S,TIMMER L W.Molecular characterization of slow-growing orangeand key lime anthracnose strains of Colletotrichum from citrus as Colletotrichum acutatum[J]. Phytopathology, 1996, 86(5):523-527.
[23] MORIWAKI J, SATO T, TSUKIBOSHI T.Morphological and molecular characterisation of Colletotrichum boninense sp. nov. from Japan[J]. Mycoscience, 2003, 44: 47-53.