Genetic mapping of the Osps-2 gene responsible for male sterility mutant in rice

doi:10.14088/j.cnki.issn0439-8114.2025.09.035

Abstract

Abstract: To elucidate the molecular mechanism of male sterility in rice (Oryza sativa L.) and its potential application in crop genetic improvement, a sterile mutant was obtained using EMS (ethyl methanesulfonate) mutagenesis and named Osps-2. Compared with the wild-type Nipponbare, the Osps-2 mutant did not show significant differences in vegetative growth but produced no seeds at maturity.Morphological observation revealed that almost no normal pollen was observed in the anthers of the Osps-2 mutant at the mature stage, indicating that its sterility phenotype might originate from abnormal pollen development. Cytological analysis found that after release from the tetrad, the microspores of wild-type Nipponbare could successfully complete subsequent development and eventually form mature pollen. In contrast, the microspores of the Osps-2 mutant degraded after release from the tetrad, accompanied by gradual shrinkage of the pollen sacs and no pollen formation. Through the screening of sterile individuals in the F2 segregation population and genetic analysis with molecular markers, the Osps-2 gene was successfully mapped to a region between 18 809 kb and 20 269 kb on chromosome 10 of rice.

Key words: rice (Oryza sativa L.), male sterility, mutant, Osps-2 gene, genetic mapping

CLC Number:

S511

GAN Tian, CAI Kai, TU Ze-yan, WANG Chao. Genetic mapping of the Osps-2 gene responsible for male sterility mutant in rice[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 229-237.

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