Physicochemical characterization of nano-selenium synthesized by Bacillus and its application in selenium-enriched tomato cultivation

doi:10.14088/j.cnki.issn0439-8114.2026.02.017

Abstract

Abstract: A SeNPs-synthesizing Bacterium MC-3 was isolated from serenium-rich soil and identified through morphological observation and 16S rDNA sequencing analysis. The physicochemical characteristics of SeNPs synthesized by strain MC-3 were analyzed by scanning electron microscope(SEM), energy dispersive spectrometer(EDS), X-ray diffraction(XRD), and Fourier transform infrared spectroscopy(FTIR), and SeNPs were applied to the cultivation experiment of selenium-enriched tomatoes. The results showed that strain MC-3 was Bacillus cereus. The SeNPs from reduction of Na₂SeO₃ by B. cereus MC-3 presented both crystalline and amorphous selenium nanoparticles, with a diameter range of 100~300 nm, and their molecule contain elements such as C, O, and Se, as well as functional groups such as -OH, C=O, and C-O, which were related to the stability of SeNPs. The SeNPs synthesized by B. cereus MC-3 were used as foliar fertilizer in the cultivation of selenium-enriched tomatoes. When the concentration of SeNPs was 70 mg/L, the selenium content in tomato fruits could reach 0.090 mg/kg FW. Meanwhile,the contents of soluble protein and vitamin C in the fruits were 41.25% and 35.00% higher than those in the control group, respectively. In conclusion, the appropriate concentration of SeNPs could be sprayed as foliar fertilizer for selenium-enriched tomato cultivation, and could improve the quality of tomato fruit.

Key words: Bacillus cereus, nano-selenium, physicochemical characterization, selenium-enriched tomato

CLC Number:

Q939.124

MA Di, SHENG Qi, KAN Guo-shi, CHEN Hong-man. Physicochemical characterization of nano-selenium synthesized by Bacillus and its application in selenium-enriched tomato cultivation[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 113-119.

References

[1] ZHANG L,ZENG H W,CHENG W H.Beneficial and paradoxical roles of selenium at nutritional levels of intake in healthspan and longevity[J]. Free radical biology and medicine,2018,127: 3-13.
[2] ANGAMUTHU A,VENKIDUSAMY K,MUTHUSWAMI R R.Synthesis and characterization of nano-selenium and its antibacterial response on some important human pathogens[J]. Current science,2019,116(2): 285-290.
[3] GB 28050—2025,食品安全国家标准预包装食品营养标签通则[S].
[4] SHI Z M,PAN P J,FENG Y W,et al.Environmental water chemistry and possible correlation with Kaschin-Beck Disease (KBD) in northwestern Sichuan,China[J]. Environment international,2017,99: 282-292.
[5] DENG X F,ZHAO Z Q,ZHOU J J,et al.Compositional analysis of typical selenium ore from Enshi and its effect on selenium enrichment in wetland and dryland crops[J]. Plant soil,2018,433(1-2): 55-64.
[6] 余侃,段炼,黄思思,等.微生物制备纳米硒的研究进展[J]. 中南农业科技,2024,45(12): 211-217.
[7] DING Q T,CUI Z W,HUANG J,et al.Selenium distribution in the Chinese environment and its relationship with human health: A review[J]. Environment international,2018,112:294-309.
[8] IZYDORCZYK G,LIGAS B,MIKULA K,et al.Biofortification of edible plants with selenium and iodine-A systematic literature review[J]. Science of the total environment,2021,754: 141983.
[9] NIE X L,YANG X R,HE J Y,et al.Bioconversion of inorganic selenium to less toxic selenium forms by microbes: A review[J]. Frontiers in bioengineering and biotechnology,2023,11: 1167123.
[10] SARKAR J,DEY P,SAHA S,et al.Mycosynthesis of selenium nanoparticles[J]. Micro and nano letters,2011,6(8): 599-602.
[11] VETCHINKINA E,LOSHCHININA E,KURSKY V,et al.Reduction of organic and inorganic selenium compounds by the edible medicinal basidiomycete Lentinula deodes and the accumulation of elemental selenium nanoparticles in its mycelium[J]. Journal of microbiology,2013,51(6):829-835.
[12] MUKHOPADHYAY S K,CHATTERJEE S,GAURI S S,et al.Isolation and characterization of extracellular polysaccharide Thelebolan produced by a newly isolated psychrophilic Antarctic fungus Thelebolus[J]. Carbohydrate polymers,2014,104(1):204-212.
[13] KIELISZEK M,SAPAZHENKAVA K.The promising role of selenium and yeast in the fight against protein amyloidosis[J]. Biological trace element research,2025,203(3): 1251-1268.
[14] HAJI MEHDI NOURI Z,TAFVIZI F,AMINI K,et al. Enhanced induction of apoptosis and cell cycle arrest in MCF-7 breast cancer and HT-29 colon cancer cell lines via low-dose biosynthesis of selenium nanoparticles utilizing Lactobacillus casei[J]. Biological trace element research,2023,202(3):1288-1304.
[15] KHALEDIZADE E,TAFVIZI F,JAFARI P.Anti-breast cancer activity of biosynthesized selenium nanoparticles using Bacillus coagulans supernatant[J]. Journal of trace elements in medicine and biology,2024,82: 127357.
[16] NILE S H,THOMBRE D,SHELAR A,et al.Antifungal properties of biogenic selenium nanoparticles functionalized with nystatin for the inhibition of Candida albicans biofilm formation[J]. Molecules,2023,28(4): 1836.
[17] AMERI A,SHAKIBAIE M,ALI FARAMARZI M,et al.Hotocatalytic decolorization of bromothymol blue using biogenic selenium nanoparticles synthesized by terrestrial actinomycete Streptomyces griseobrunneus strain FSHH12[J]. Desalination and water treatment,2016,57(45): 21552-21563.
[18] 张琪,李亚妮,周荣娟,等.阿维链霉菌合成纳米硒的物理化学表征及其对枸杞病原真菌的抑菌活性[J]. 生物工程学报,2025,41(2): 693-705.
[19] RADHAKRISHNAN R,HASHEM A,ABD_ALLAH EF. Bacillus: A biologicao tool for crop improvement through bio-molecular changes in adverse environments[J]. Frontiers in physiology,2017,8: 667-669.
[20] 马丽莎,陈小玲,檀艳萍,等.一株高耐硒雷氏普罗威登斯菌的分离鉴定[J]. 食品科技,2023,48(5): 1-9.
[21] 张立秋,向建伟,胡琴,等.利用地衣芽孢杆菌制备纳米硒能力的研究[J]. 山东化工,2023,52(8): 13-16.
[22] 朱燕云,王欣,陈丹艳,等.芽孢杆菌生物合成纳米硒条件优化及活性评价[J]. 农业工程学报,2023,39(1):269-274.
[23] LU S,NA K,LI Y R,et al.Bacillus-derived probiotics: Metabolites and mechanisms involved in bacteria-host interactions[J]. Critical reviews in food science and nutrition,2024,64(6):1701-1714.
[24] 张一雯,迟凤琴,张久明,等.喷施外源硒对番茄和西瓜果实硒含量及品质的影响[J]. 北方园艺,2021(2): 47-52.
[25] 邵旭日,韩莹琰,齐长红,等.叶面施用不同浓度的硒肥对番茄果实品质的影响[J]. 蔬菜,2017(8): 25-28.
[26] 李利军,马英辉,卢美欢. 纳米硒合成细菌Lxz—41的鉴定、培养条件优化及其在富硒猕猴桃栽培中的应用[J]. 食品工业科技,2019,40(21):110-117.
[27] 白玲,霍群. 基础生物化学实验[M]. 第二版. 上海:复旦大学出版社,2008.
[28] 刘绍俊,牛英,刘冰浩,等.钼蓝比色法测定沙田柚果肉中还原型维生素C含量的研究[J]. 北方园艺,2011(1): 8-12.
[29] YANG F,TANG Q M,ZHONG X Y,et al.Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles[J]. International journal of nanomedicine,2012,7: 835-844.
[30] 袁莲莲,刘天波,李晓东,等.多黏类芽孢杆菌抗真菌活性及其纳米硒化应用[J]. 河南农业科学,2025,54(1):101-108.
[31] 朱燕云,孔祥平,吴娥娇,等.耐高盐枯草芽孢杆菌XP合成球形纳米硒及其抑制草莓病原真菌生物活性[J]. 生物工程学报,2021,37(8): 2825-2835.