Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain

doi:10.14088/j.cnki.issn0439-8114.2025.05.005

Abstract

Abstract: To better utilize microorganisms for environmental bioremediation, reduce selenium pollution in soil, and rationally exploit selenium resources, a selenium-tolerant strain, ZJ2, was isolated from selenium-rich soil in Yutangba, Enshi City, Hubei Province. Molecular biological identification confirmed it as Bacillus cereus. This strain exhibited high tolerance and reduction capability to sodium selenite (Na₂SeO₃), converting it into stable biogenic selenium nanoparticles (SeNPs). Additionally, the effects of four different carbon sources on the sodium selenite reduction efficiency and the stability of synthesized SeNPs were investigated. The results showed that strain ZJ2 had a 92% reduction capacity to 5 mmol/L sodium selenite. The bio-nanoselenium synthesised by this strain was spherical particles with an average particle size of (188.42±2.63) nm and a Zeta potential of (-41.17±1.77) mV. It was found that the addition of rhamnose and glucose promoted the reduction of sodium selenite by strain ZJ2, with an increase in the reduction rate of 37.48% and 39.53%, respectively. In addition, rhamnose had a good promoting effect on the synthesis of stable selenium nanoparticles by the strain. Based on a comprehensive evaluation of the sodium selenite reduction capability, characterization of selenium nanoparticles (SeNPs) and their stability, it was demonstrated that the addition of rhamnose significantly enhanced the ability of Bacillus cereus ZJ2 to efficiently synthesize more stable biogenic SeNPs.

Key words: selenium-resistant strain, Bacillus cereus, nano-selenium, reduction rate, stability, carbon source

CLC Number:

S154.3

HE Jia-li, LIU Meng-qi, ZHONG Wen-yi, HE Yi, WANG Zhang-qian, CHENG Shui-yuan. Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 25-32.

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