Analysis of genetic stability of rice transformed with sm-Ngt1

doi:10.14088/j.cnki.issn0439-8114.2022.04.004

Abstract

Abstract: To detect the genetic stability of the offspring of the transgenic with sm-Ngt1 plant lines, 15 T4 rice lines transformed with sm-Ngt1 gene were planted, the positive plants were identified, the genetically modified markers were tested with 9 primer pairs required by the ministry of agriculture and the agronomic trait was counted for two consecutive years. The results showed that the sm-Ngt1 was found in 11 genetically modified rice lines numbered 1, 3, 5, 7-9, 11-15 in the 15 lines, and in the another 10 lines except numbered 8 the sm-Ngt1 was genetically stable. There were 5 of 9 primer pairs of detecting markers with amplification strips, of which there were 3 primer pairs NOS-F1/NOS-R1, T35S-F1/T35S-R1, NPTF68/NPTR356 with specific bands in all genetically modified offspring （T5 and T6 generations）, and the test results for 2 primer pairs （35S-F1/35S-R1and HPTF226/HPTF69） were the same as for sm-Ngt1. These results demonstrated that the 10 genetically modified rice lines numbered 1, 3, 5, 7, 9, 11-15 contained the 35S-sm-Ngt1 and HPT were able to maintain stable genetics, and there was no significant difference in growth period, plant height, seeding rate and thousand grain weight between the modified lines with sm-Ngt1 and the receptor.

Key words: sm-Ngt1, rice, genetic stability, agronomic trait

CLC Number:

S188

XIA Dong, LU Wei, ZHANG Jun, TAN Yan-ping, LIU Xue-qun, WANG Chun-tai. Analysis of genetic stability of rice transformed with sm-Ngt1 [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(4): 24-28.

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