Identification of the EIN3/EIL gene family in foxtail millet and its expression analysis in multiple tissues

doi:10.14088/j.cnki.issn0439-8114.2026.07.033

Abstract

Abstract: In order to systematically elucidate the evolutionary characteristics, structural features, and potential regulatory functions of the EIN3/EIL (SiEIN3/EIL) gene family in foxtail millet (Setaria italica) during growth, development, and environmental adaptation, a systematic identification and multi-tissue expression profile analysis were conducted based on the whole-genome data of foxtail millet. Through combined HMMER and BLASTP searches, a total of four EIN3/EIL family members were identified in foxtail millet, with gene IDs of Seita.1G205600.1, Seita.7G121700.1, Seita.2G437500.1, and Seita.9G419900.1. Bioinformatics analysis indicated that all proteins of this family were localized in the nucleus and were hydrophilic, distributed dispersedly across four chromosomes; all members contained exons, shared six core conserved motifs, and their promoter regions were enriched with cis-acting elements associated with light responses, multiple phytohormone responses (e.g., methyl jasmonate, abscisic acid), and abiotic stress responses. Expression analysis of multi-tissue transcriptome data revealed differences in the expression levels among the genes, among which Seita.9G419900.1 exhibited significantly higher expression from the jointing stage to the flowering stage than during vegetative growth stages such as the seedling stage, playing a core regulatory role mainly in reproductive development and floral organ formation.

Key words: foxtail millet (Setaria italica), EIN3/EIL gene family, identification, tissue, bioinformatics, expression analysis

CLC Number:

S515

LIU Peng-cheng, WANG Ya-yu, YANG Tian-tian. Identification of the EIN3/EIL gene family in foxtail millet and its expression analysis in multiple tissues[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(7): 215-220.

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