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Table of Content

    25 July 2026, Volume 65 Issue 7
    Breeding & Cultivation
    Differential responses of different genotypes of rapeseed to gibberellin regulation
    ZHANG Ying-ying, LI Mei, LUO Li-ya, ZHU Pei, XU Ben-bo, XU Jin-song, ZHANG Xue-kun
    2026, 65(7):  1-5.  doi:10.14088/j.cnki.issn0439-8114.2026.07.001
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    Twelve rapeseed cultivars(lines) belonging to three types were used as experimental materials. Four treatments were established, including 40 mg/L uniconazole, 50 mg/L paclobutrazol,18.75 mg/L GA3 and 30 mg/L GA4. Spraying was conducted on the 7th day after rapeseed germination. Agronomic traits at the seedling and mature stages were determined, and cold tolerance and waterlogging tolerance of seedlings were evaluated. The results showed that the coefficient of variation for waterlogging tolerance was the highest. Treatments with uniconazole and paclobutrazol significantly reduced seedling height and main root length, and markedly increased lateral root number and leaf chlorophyll content. By contrast, GA3 and GA4 treatments substantially increased seedling height and main root length, but significantly inhibited lateral root number and leaf chlorophyll content. Overall, the sensitivity of seedling agronomic traits of different rapeseed genotypes to uniconazole, paclobutrazol and gibberellins ranked from high to low as: Chinese semi-winter female parent × winter male parent, Chinese semi-winter female parent × (Chinese semi-winter × European winter) DH male parent, and Chinese semi-winter female parent × Chinese semi-winter male parent. Stepwise regression analysis was performed between the response values of seedling traits to gibberellin treatments and seedling stress resistance as well as yield. The results indicated that yield-related traits had no correlation with the response values of seedling traits to gibberellins. The response value of seedling chlorophyll content to GA4 was closely associated with seedling cold tolerance, and the response value of seedling chlorophyll content to GA3 was significantly correlated with growth period. Additionally, the response values of root length and lateral root number to paclobutrazol were strongly related to waterlogging tolerance. In conclusion, rapeseed genotypes presented distinct differences in response to gibberellins. In practical production, gibberellin-related plant growth regulators could be adopted for classified regulation of seedlings of different rapeseed cultivars, so as to precisely improve the stress resistance of rapeseed seedlings.
    Preliminary study on wheat × maize induced haploid breeding technology in Northern Xinjiang
    ZENG Tian-yun, ZHU Yu, LI Hui-qin, ZHANG Wen-tao, GUO Zong-zong, WANG Qian, LIN Xia
    2026, 65(7):  6-11.  doi:10.14088/j.cnki.issn0439-8114.2026.07.002
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    To establish a technical system for inducing wheat haploids via wheat-maize hybridization suitable for the Northern Xinjiang wheat-growing region, three winter wheat F1 hybrids and three spring wheat F1 hybrids were selected as female parents, and early-maturing sweet-waxy maize varieties of different types were introduced as male parents for pollination to induce haploid embryo formation in wheat. The difference of seed setting rate and haploid embryo recovery rate of different genotypes of wheat induced by maize under different sowing dates in the greenhouse was studied. The results showed that under greenhouse cultivation, the hybridization period for winter wheat and maize was mid-to-late May, while that for spring wheat and maize ranged from late May to early June, with a suitable duration of 7-14 days when sufficient maize pollen was available. The pollen rolling pollination method was applicable in this period. The seed setting rate of hybrid combinations ranged from 18.82% to 76.14%, and the embryo recovery rate was 1.41%-20.00%. The combining ability was ranked as Baitiannuo > Tiannuo 208-B3 > Wannuo. The average seed setting rate and average embryo recovery rate reached 39.79% and 6.68% with single pollination, respectively, while those increased to 53.91% and 10.77% with double pollination, indicating that double pollination significantly increased seed setting rate and embryo recovery rate. The average embryo recovery rate of plump hybrid caryopses was 5.84%, which was 2.17 times higher than that of shrivelled caryopses.
    Effects of ethephon ripening acceleration on yield and quality of rape
    JIN Di, LEI Ran, AI Tang-shun, QIN Huan, LIU Long, WU Ya-bin
    2026, 65(7):  12-16.  doi:10.14088/j.cnki.issn0439-8114.2026.07.003
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    To explore the effects of ethephon ripening acceleration on yield and quality of different rapeseed (Brassica napus L.) cultivars, three rapeseed varieties/lines (Shuangyou 195, PW-1B and 19FW-1) were used as experimental materials. Four ethephon concentrations (500, 750, 1 000 and 2 000 mg/kg) and three spraying dates (17 May, 20 May and 22 May) were arranged for the ripening-accelerating trial. After harvest, the moisture contents of pod husks, seeds and straws, as well as yield and quality traits of plants were determined. The results showed that except for a few treatments, the moisture contents of pod husks, straws and seeds of three rapeseed varieties/lines were markedly lower than those of the control. In terms of germination rate, all treated Shuangyou 195 samples maintained a germination rate above 90%. No significant differences in germination rate of 19FW-1 were detected between ethephon treatments and the control, whereas low-concentration ethephon applied at the early stage significantly impaired the germination rate of PW-1B. The grain yield of all tested varieties/lines declined to varying extents, with cultivar-specific discrepancies among genotypes. Quality analysis showed that after ethephon spraying for Shuangyou 195, the contents of high oleic acid, oleic acid, linolenic acid, palmitic acid and stearic acid decreased, while the contents of linoleic acid and protein increased. For PW-1B, the contents of glucosinolate, high oleic acid, linolenic acid, palmitic acid and stearic acid were reduced, whereas linoleic acid and protein contents were elevated. For 19FW-1, the contents of glucosinolate, high oleic acid, oleic acid, linolenic acid, protein, palmitic acid and stearic acid decreased, and only the linoleic acid content increased. Considering the comprehensive performance of yield and quality, ethephon could be practically applied for ripening acceleration in rapeseed production. For the cultivar Shuangyou 195, 2 000 mg/kg ethephon was recommended to be sprayed at 7-8 days before harvest with appropriately advanced harvesting time. For the colored rapeseed lines PW-1B and 19FW-1, spraying concentrations of 2 000 mg/kg and 1 000 mg/kg ethephon at 5-6 days before harvest were the optimal treatments, respectively.
    Screening and comprehensive evaluation of low-Cd accumulating maize varieties in heavy metal-contaminated farmland of Northwestern Hubei
    XU Zi-gang, QIN Dian-feng, ZHOU Hong-yu, YANG Li, LIU Bo, SONG Na, HONG Jun
    2026, 65(7):  17-22.  doi:10.14088/j.cnki.issn0439-8114.2026.07.004
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    To screen maize (Zea mays L.) varieties with high yield potential and low-cadmium (Cd) accumulation for heavy metal-contaminated farmland in Northwest Hubei, a randomized block experiment was conducted on a heavy metal-contaminated farmland using 15 maize varieties. The agronomic traits, yield, and the contents of cadmium (Cd), lead (Pb), chromium (Cr), arsenic (As) and mercury (Hg) in grain of all varieties were determined. One-way analysis of variance (ANOVA) and the LSD method were adopted to compare differences among varieties. The entropy weight-TOPSIS model was applied for multi-index comprehensive evaluation and ranking, and Pearson correlation analysis was used to explore the relationships between agronomic traits and grain Cd accumulation. The results showed that extremely significant differences (P<0.001) existed in grain yield and grain Cd content among different maize varieties. The average yield ranged from 5.48 to 7.66 t/hm2, among which Tongyu 609, Tongyu 808 and Mengyu 298 presented relatively high yields. The top five varieties in the comprehensive evaluation by the entropy weight-TOPSIS model were Weike 702, Mengyu 298, Tongyu 808, Huayu 130 and Aiheyu 501. These superior varieties generally featured large biomass and robust stalks. Correlation analysis indicated that key above-ground agronomic traits (plant height, biomass) had no significant correlation with grain Cd accumulation. In conclusion, varieties including Weike 702, Mengyu 298 and Tongyu 808 exhibited high yield and low heavy metal accumulation in heavy metal-contaminated farmland of Yunxi County. They were suitable for popularization and cultivation in this area and other similarly contaminated farmlands.
    Genetic diversity analysis of wheat varieties bred by Xiangyang Academy of Agricultural Sciences
    SUN Hua-wei, LING Dong, ZHANG Dao-rong, TANG Qing-yi, ZHOU Fang-ju, TANG Qing, LIU Xian-bin, WANG Zhi-shun, WANG Xin, TANG San-ming
    2026, 65(7):  23-27.  doi:10.14088/j.cnki.issn0439-8114.2026.07.005
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    In order to identify the genetic diversity of wheat varieties bred by Xiangyang Academy of Agricultural Sciences and to guide parent selection, a genome-wide scan was conducted on 28 wheat varieties using the 100k SNP chip to analyze their genetic diversity. The results showed that the number of polymorphic mSNP loci was the highest on chromosome 2A (10 982) and the lowest on chromosome 4D (1 469). The distribution of polymorphic loci was relatively uniform on homologous chromosome groups, with the most on chromosome B and the least on chromosome D. The polymorphic information content (PIC) ranged from 0.096 to 0.375, with an average of 0.262. The genetic distances between the test materials ranged from 0.002 38 to 0.421 68, with an average of 0.305 84, and were mainly concentrated between 0.25 and 0.40, accounting for 70.63% of the total. Xiangmai 93 and Xiangmai 21 shared the closest genetic distance (0.002 38), while the maximum genetic distance (0.421 68) was observed between Xiangmai 1816 and Xiangmai 93. The 28 varieties were divided into three groups based on cluster analysis, with some varieties in the same cluster having relatively close genetic distances. Overall, the genetic diversity of the selected varieties was low, and their genetic backgrounds were similar. Therefore, it was necessary to strengthen the introduction of superior exogenous genes during variety selection.
    Breeding and application of Yi110S, a high-quality indica rice photo-thermo-sensitive genic male sterile line
    LI Xing-hua, CAI Xing-xing, TU Jun-ming, ZHANG Sheng, WANG Huan, CHEN Jie, CAO Zhi-gang, ZHANG Wen-chao, DENG Qiu-yu, HUANG Jin-qiu
    2026, 65(7):  28-31.  doi:10.14088/j.cnki.issn0439-8114.2026.07.006
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    The breeding process, characteristic traits and hybrid application performance of Yi110S, a high-quality indica rice photo-thermo-sensitive genic male sterile (TGMS) line, were systematically elaborated. Yi110S was bred by Huanggang Academy of Agricultural Sciences through crossing the self-bred sterile line 46S as the female parent with the high-quality restorer line GR347. In the F2 generation, sterile plants were selected and backcrossed with GR347. Following the breeding strategy of selecting fertile plants with superior grain quality in early generations and screening for stable sterile plants in advanced generations, Yi110S was bred through systematic multi-generation selection over years. This TGMS line exhibited moderate plant architecture, uniform panicle distribution, stable fertility conversion temperature, favorable flowering habit, strong combining ability and excellent grain quality. It was certified by the Hubei Provincial Crop Variety Approval Committee in 2025. The hybrid combination Yiliangyou 1046, derived from Yi110S, demonstrated suitable growth duration, high yield potential, superior grain quality and good resistance to rice blast, and had passed the regional trials and production trials for early-maturing medium rice in Hubei Province in 2025, showing promising application prospects.
    Resource & Environment
    Effects of intercropping dandelion on soil fertility, enzyme activities, and microbial community structure in apple orchards
    TIAN Qi, WU Jin-shui, LI Tian, XI Ming-pan, ZHANG Ding-jun, HU Chun-yan, CAO Hui
    2026, 65(7):  32-42.  doi:10.14088/j.cnki.issn0439-8114.2026.07.007
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    To elucidate the regulatory effects of orchard intercropping dandelion(Taraxacum mongolicum Hand.-Mazz.) on the soil micro-ecological environment and system vitality, this study was conducted in an apple orchard in Qingcheng County, Qingyang City, Gansu Province. Clean cultivation (CK) and intercropping dandelion (PGY) treatments were established, and soil physicochemical properties, enzyme activities, and microbial community characteristics were systematically determined. The results showed that from the young fruit stage to post-harvest, the available phosphorus and readily available potassium contents in the clean cultivation plot exhibited a significant decreasing trend; in the intercropping plot, the readily available potassium content exhibited a significant decreasing trend, whereas the total nitrogen, total phosphorus, alkali-hydrolyzable nitrogen, available phosphorus, and organic matter contents all exhibited significant increasing trends compared with those at the young fruit stage. Intercropping dandelion effectively maintained the relative stability of the activities of key soil enzymes, including catalase, urease, and acid phosphatase. Microbial community analysis revealed that intercropping dandelion effectively maintained the relative stability of bacterial and fungal community richness (ACE and Chao1 indices) in the orchard soil, significantly promoted the proliferation of beneficial microbial groups such as Schizothecium, and suppressed the enrichment of potentially soil-borne pathogenic genera such as Leptosphaeria and Volutella. The Mantel test and correlation analysis indicated that soil readily available nutrients and enzyme activities were the key environmental factors driving the succession of microbial communities. In summary, intercropping dandelion effectively alleviated the risks of soil functional degradation and continuous cropping obstacles caused by long-term clean cultivation by ameliorating rhizosphere hydrothermal conditions, continuously supplying exogenous organic matter, and broadening microbial niches.
    Comprehensive evaluation of plant selenium enrichment capacity and screening of high-potential selenium-enriched plants in selenium-rich area of Ankang City
    DU Xiao-ping, WANG Qi, ZHU Ai-min, ZHANG Jun, ZHAO Jian-xiang, LIU Jia-long, ZHOU Chen-yao, ZHOU Dan-wen, ZHANG Ying, JING Lin-fei, WEI Xin-yi
    2026, 65(7):  43-50.  doi:10.14088/j.cnki.issn0439-8114.2026.07.008
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    To screen plant resources with high selenium accumulation potential in selenium-rich areas of Ankang City, Shaanxi Province, a total of 121 sets of plant and rhizosphere soil samples were collected. Selenium content, translocation factor (TF), bioconcentration factor (BCF) and other indices were determined, and the selenium accumulation capacity of plants was comprehensively evaluated by the membership function method and cluster analysis. The results showed that the average selenium contents in shoots, roots and rhizosphere soil of plants in the study area were 4.30 mg/kg, 2.83 mg/kg and 12.89 mg/kg, respectively. The translocation factor of selenium was higher than 1 in 64.46% of the samples. The bioconcentration factor of selenium in shoots exceeded 1 in 11.57% of the samples, while that in roots was greater than 1 in 4.13% of the samples. Among them, the three indices of Hibiscus manihot L., Plantago asiatica L., Raphanus sativus L. and Brassica juncea (L.) Czern. were all greater than 1. Cluster analysis classified the plants into three categories by comprehensive selenium accumulation capacity: high potential plants (4.13%), moderate potential plants (9.92%) and low potential plants (85.95%). Brassica juncea (L.) Czern., Hibiscus manihot L., Sonchus asper (L.) Hill, Artemisia annua L. and Portulaca oleracea L. were identified as plants with high comprehensive selenium accumulation potential.
    Spatial pattern and influencing factor spatial heterogeneity of geological hazards in the national key ecological function zone of southwestern Hubei
    SHU Nan-nan, PAN Fang-jie
    2026, 65(7):  51-59.  doi:10.14088/j.cnki.issn0439-8114.2026.07.009
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    In order to systematically reveal the spatial distribution patterns and driving mechanisms of geological hazards in the national key ecological function zone of southwestern Hubei, and to provide a scientific basis for grassroots disaster prevention and mitigation and regional ecological security maintenance, this study took Changyang County of Hubei Province, a typical geological hazard-prone area, as a case area and investigated the spatial pattern of geological hazards and the spatial heterogeneity of influencing factors at the village scale. Standard deviational ellipse analysis, kernel density estimation, and exploratory spatial data analysis (ESDA) were comprehensively employed to characterize the spatial differentiation features of the hazards, and the geographically weighted regression (GWR) model and the geographical detector model were used to quantitatively analyze the spatial heterogeneity of the core influencing factors and their interaction mechanisms. The results showed that the geological hazards in Changyang County were mainly distributed in a banded cluster along the Qingjiang River in an east (slightly north) - west (slightly south) orientation, with strong centrality and pronounced directionality. The hazard distribution at the village scale exhibited significant spatial autocorrelation, presenting a block-like pattern of three major hot spots and two major cold spots, with the highest point density of hazards along the Qingjiang River and in densely populated areas. Road network density, relief amplitude, elevation, population density, and vegetation coverage were the main driving factors. The cold and hot spot areas exhibited a coexistence of nonlinear enhancement and bi-factor enhancement, with significant spatial differentiation in interaction intensity. This study could provide decision-making references for the precise prevention and control of geological hazards, the optimization of spatial planning, and the coordination of human-land relationships in the key ecological function zone of southwestern Hubei, and it was recommended that monitoring, early warning, and comprehensive management in hot spot areas be strengthened according to local conditions, and that the intensity of human engineering activities in highly geological hazard-sensitive areas be strictly controlled.
    Spatio-temporal characteristics and influencing factors of cultivated land non-agriculturalization in the Guangdong-Hong Kong-Macao Greater Bay Area from 2000 to 2020
    GUO Jing-chao, ZHANG Guo-zhuang
    2026, 65(7):  60-69.  doi:10.14088/j.cnki.issn0439-8114.2026.07.010
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    Under the background of rapid urbanization, it is of great practical significance to reveal the spatio-temporal differentiation characteristics and influencing factors of non-agriculturalization of cultivated land in the Guangdong-Hong Kong-Macao Greater Bay Area for coordinating economic development and cultivated land protection in highly urbanized areas. Based on the multi-period land use data from 2000 to 2020, the spatial-temporal pattern and influencing factors of non-agriculturalization of cultivated land in the Guangdong-Hong Kong-Macao Greater Bay Area were systematically analyzed by integrating natural geography and socio-economic factors, and using the change index of cultivated land quantity, spatial autocorrelation analysis and interpretable machine learning method (XGBoost-SHAP-PDP). The results showed that the area of cultivated land in the Guangdong-Hong Kong-Macao Greater Bay Area continued to decrease by 270 000 hm2 during the study period. The non-agriculturalization rate showed a stage evolution characteristic of rapid growth-fluctuating decline-slow recovery over time, and showed a significant “core-periphery” gradient differentiation pattern in space. The global Moran's I was 0.24-0.53, and the non-agriculturalization of cultivated land had significant spatial agglomeration. The high-value areas gradually expanded from the core region of Guangzhou, Foshan and Huizhou to the eastern coastal zones. The overall migration trend of the gravity center of cultivated land non-agricultural conversion followed the sequence of “southeastward-northwestward-northeastward”, and the migration distance increased from 4.24 km in 2005-2010 to 18.99 km in 2010-2015, and further expanded to 20.72 km in 2015-2020. The directional characteristic of its spatial distribution first weakened and then strengthened. In terms of influencing factors, population and economic conditions served as major drivers, while their impacts gradually declined over time. By contrast, agricultural mechanization and grain output exerted an increasingly prominent restraining effect. Complex non-linear synergistic relationships existed among various influencing factors.
    Dynamic monitoring of water surface area of Hulun Lake from 1991 to 2019 and its driving mechanism
    ZENG Wan-qiu, QIN Jie, LI Yu-ling
    2026, 65(7):  70-75.  doi:10.14088/j.cnki.issn0439-8114.2026.07.011
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    To reveal the spatiotemporal evolution of the water surface area of Hulun Lake over the past 30 years and its dominant driving factors, and to provide a scientific basis for watershed ecological protection and optimal allocation of water resources, this study used Landsat TM/OLI remote sensing images from 1991 to 2019, employed the Modified Normalized Difference Water Index (MNDWI) and GIS spatial analysis techniques to extract the time series of the lake's water surface area, and combined meteorological and hydrological data with Pearson correlation analysis to quantitatively evaluate the impacts of natural climate and human activities on changes in the lake's water surface area. The results showed that the evolution of the water surface area of Hulun Lake from 1991 to 2019 underwent three stages: a high-level fluctuation period, a continuous shrinkage period, and a recovery and adjustment period. The water surface area ranged from 1798.0 to 2 389.0 km2, with a range of 591.0 km2. There was no significant linear correlation between the water surface area of Hulun Lake and the annual mean temperature or annual precipitation. A single climatic factor could not fully explain the complex evolution of Hulun Lake over the past 30 years. The shrinkage of the water surface area of Hulun Lake from 2000 to 2010 was mainly driven by the sharp reduction in upstream inflow into the lake, especially the frequent drying-up of the Kherlen River, whereas the rapid recovery of the water surface area after 2010 was primarily attributed to the effective implementation of ecological water replenishment projects such as the “Diverting River Water to Recharge the Lake” project. The study indicated that the evolution mechanism of the water surface area of Hulun Lake had shifted from being dominated by natural hydrology to being jointly driven by natural and artificial regulation. Therefore, it was recommended that the joint dispatch of water resources across the entire basin and the dynamic ecological water replenishment mechanism be strengthened in the future, so as to ensure the long-term stability of the lake ecosystem.
    Analysis of carbon emission effects and multi-scenario prediction based on land use in Karamay City
    ZENG Wei-peng, HE Liang, WANG Jie, CHEN Shuo
    2026, 65(7):  76-83.  doi:10.14088/j.cnki.issn0439-8114.2026.07.012
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    To optimize the land use pattern of resource-based cities in arid regions and synergistically promote low-carbon transition and ecological protection, this study took Karamay City as the research object and systematically explored the spatiotemporal evolution characteristics, driving mechanisms, and future evolution trends of its land use carbon emissions. The carbon emission coefficient method, Tapio decoupling model, and LMDI decomposition model were comprehensively applied to evaluate the carbon emission effects of land use from 2000 to 2020, and the PLUS model was coupled with the STIRPAT model to simulate and predict the land use pattern and carbon emission trends under four scenarios for 2030, natural development, ecological protection, cultivated land protection, and urban development. The results showed that land use in Karamay City from 2000 to 2020 exhibited a “five increases and one decrease” trend, with net carbon emissions nearly doubling. Construction land was the core carbon source, while the carbon sink function of ecological land such as forestland and grassland was weak. Carbon emissions and economic development showed an overall weak decoupling relationship. The economic output effect and the construction land scale effect were the main driving forces for the increase in carbon emissions, while the energy efficiency effect was the key inhibiting factor. The multi-scenario prediction for 2030 showed that under the ecological protection scenario, the expansion of construction land was effectively suppressed and net carbon emissions were reduced to the lowest level. The development model corresponding to this scenario was the optimal path that gave consideration to both ecological protection and carbon emission reduction.
    Plant Protection
    Eclosion and reproductive behavior of Archips micaceana
    CHEN Wen-jia, TANG Xuan, PENG Yi-qin, LI Jun-feng, QIN Liu-xia, YU Yong-hui
    2026, 65(7):  84-89.  doi:10.14088/j.cnki.issn0439-8114.2026.07.013
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    To clarify the adult emergence and reproductive behaviors of Archips micaceana, laboratory rearing was conducted to observe the rhythms and behaviors of emergence, calling, mating, and oviposition. The results showed that the emergence, calling, and mating behaviors of Archips micaceana exhibited significant photoperiodic rhythms. The peak emergence of females occurred from 17:00 to 18:00, while that of males occurred from 16:00 to 17:00; the overall emergence peak was from 17:00 to 18:00, with females emerging approximately one day earlier than males. The calling peak occurred within one hour after the onset of the photophase, with an average calling duration of 252.23 minutes. The pre-mating period for both sexes was two days. The mating peak occurred within 1-3 hours after the onset of the photophase, with an average mating duration of 111.97 minutes. Each female mated an average of 2.37 times during its lifetime, whereas each male mated an average of 3.80 times. Females began oviposition on the night after mating, with the oviposition peak occurring one hour after the onset of the scotophase, and the average fecundity per female was 502.33 eggs.
    Pathogen identification, biological characteristics, and laboratory fungicide screening of leaf blight on Curcuma wenyujin in Guangxi
    ZHAN Xin-jie, LIANG Li-yi, YANG Dong-yu, JIANG Ni, HUANG Qi, WEI Shu-gen, SONG Li-sha, BAI Dan-yu
    2026, 65(7):  90-96.  doi:10.14088/j.cnki.issn0439-8114.2026.07.014
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    To clarify the causal agent of leaf blight on Curcuma wenyujin in Guangxi, identify the pathogenic species, analyze its biological characteristics, and evaluate the efficacy of common fungicides, the pathogen was isolated via the tissue isolation method. Species identification was conducted through pathogenicity tests, morphological observation, and multi-gene sequence analysis of ITS, TEF1, H3, and TUB2. The effects of different media, temperature, pH, light, and carbon/nitrogen sources on mycelial growth were determined using the mycelial growth rate method. The indoor toxicity of four fungicides was tested using the fungicide-amended plate method. A total of 38 strains were isolated and purified from diseased leaf tissues. The 27 strains with the highest isolation frequency (71.05%) and consistent morphology were selected as representative strains and designated JH43-2 for subsequent studies. Morphological and multi-gene phylogenetic analyses identified strain JH43-2 as Diaporthe ueckeri. The fungus grew optimally at 20-30 ℃, tolerated pH 5-9, and utilized various carbon and nitrogen sources, with the most vigorous mycelial growth observed when inositol was used as the carbon source and sodium nitrate as the nitrogen source. Toxicity assays showed that the tested agents—Trichoderma harzianum, hymexazol, pyraclostrobin, and prochloraz—all inhibited the pathogen, with Trichoderma harzianum exhibiting the strongest effect (EC50= 0.019 6 mg/L).
    Identification and biological characteristics of the pathogen causing anthracnose on Cinnamomum cassia in Yulin, Guangxi
    MENG Hai-qin, LU Chen-sheng, CHEN Xing-wen, QIN Hao-min, HUANG Guang-lan, YAN Rong-bin, WU Jun
    2026, 65(7):  97-104.  doi:10.14088/j.cnki.issn0439-8114.2026.07.015
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    To clarify the pathogen species causing anthracnose on Cinnamomum cassia in Yulin, Guangxi, and its biological characteristics, the pathogen was isolated and purified from diseased leaves using the tissue isolation method. It was identified through pathogenicity testing, morphological observation, and concatenated phylogenetic analysis based on four gene fragments (ITS, ACT, GAPDH, and TUB2). The effects of carbon sources, nitrogen sources, temperature, light, and pH on mycelial growth were also investigated. The results showed that the pathogen causing anthracnose on Cinnamomum cassia in Yulin was Colletotrichum fructicola. This study reported for the first time that Colletotrichum fructicola infected Cinnamomum cassia. The optimal carbon source for mycelial growth was maltose, and the optimal nitrogen sources were ammonium dihydrogen phosphate and peptone. The optimal temperature for growth was 28 ℃, with the best growth observed under continuous light and at pH 6. The lethal condition for the mycelia was treatment at 52 ℃ for 15 min.
    Development of a triolein-based botanical pesticide and its laboratory efficacy evaluation
    SHI Qi-wen, GAO Jing, YE Feng-xian, WANG Zhao-hong, Clement Nzabanita, WU Zhong-zhen, MAO Run-qian
    2026, 65(7):  105-109.  doi:10.14088/j.cnki.issn0439-8114.2026.07.016
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    Using triolein, the main active component of vegetable oils, as the target compound, a novel green pesticide was developed, and its formulation performance, insecticidal activity, and growth-regulating effects on crops were evaluated. The optimal hydrophilic-lipophilic balance (HLB) was determined through emulsifier screening and compounding, and the emulsification system was optimized to prepare a 90% triolein emulsion. The toxicity of the emulsion against adult whiteflies (Bemisia tabaci) was evaluated using the immersion method, its repellent activity was tested with a Y-tube olfactometer, and its growth-promoting effects on tomato seedlings were investigated through pot experiments. The results showed that the optimal HLB for triolein was 8.5. A composite emulsifier consisting of AEO3, NP4, and Tween-80 at a mass ratio of 1∶1∶0.015, added at a concentration of 10%, produced an emulsion with excellent emulsification properties. The emulsion exhibited significant contact toxicity against adult whiteflies, with an LC50 of 167.16 mg/L, which was significantly lower than that of the control SK mineral oil (LC50 = 354.54 mg/L). In addition, the emulsion showed a repellent effect against whiteflies, with repellency rates of 76.00% and 64.37% at 1 hour and 1 day after application, respectively, and no significant differences were observed between the triolein emulsion and the SK mineral oil control at any time point. At a concentration of 5.00 mg/L, triolein consistently promoted the growth of tomato seedlings, with both the relative growth rate of plant height and the net increase in stem diameter being significantly higher than those of the blank control after multiple treatments. In contrast, the same concentration of SK mineral oil significantly inhibited the net increase in tomato stem diameter.
    Horticulture & Local Products
    Effects of different colored plastic films on quality and yield of dwarf common bean
    PENG Yin, ZHANG Jian-hua, SUN Xin-cheng, HUANG Rong-rong, YUE Feng, TIAN Ju-ying, BAI Wei-tao, JIANG Wan, YANG Lian-yong, HUANG You-hua
    2026, 65(7):  110-113.  doi:10.14088/j.cnki.issn0439-8114.2026.07.017
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    To investigate the effects of different colored plastic film mulching on photosynthetic pigment content, fruit quality and yield of dwarf common bean (Phaseolus vulgaris L.), and to select suitable film types for high-quality and efficient cultivation in the Yangtze River Basin, the self-bred dwarf common bean variety Aijiang 321 was used as the test material. Four colored films, i.e., red (R), blue (B), purple (P) and yellow (Y), were applied, with colorless film as the control (CK). The contents of chlorophyll a, chlorophyll b, and carotenoids in leaves at the mature stage, as well as the contents of free amino acids, vitamin C, soluble sugar, soluble protein and yield in pods were determined. The results showed that under yellow film treatment, the contents of chlorophyll a, chlorophyll b and chlorophyll a+b were the highest, which were significantly increased by 26.29%, 38.10% and 28.57%, respectively, compared with CK. The photosynthetic pigment contents under blue and purple films were also significantly higher than those under CK, while no significant difference was observed between red film treatment and CK. Regarding fruit quality, the contents of free amino acids and soluble protein in all colored film treatments were significantly lower than those in CK, with a greater reduction under yellow film. No significant differences were found in vitamin C and soluble sugar contents among all treatments. In terms of yield, the yield per plant and yield per plot under all colored film treatments were significantly lower than those of CK. Among them, purple film treatment gave the relatively highest yield, yet still decreased by more than 10% compared with CK, while red and blue films caused a yield reduction of over 20%.
    Effects of different fermentation media on the quality of Yunnan-produced cigar tobacco leaves
    ZHAO Zhi-liang, YANG Wan-long, SHI Man, HU Yong-jin, LI Bo, Hu Jian-yu, HE Xiao-hui
    2026, 65(7):  114-122.  doi:10.14088/j.cnki.issn0439-8114.2026.07.018
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    To investigate the influence of different fermentation media on the quality characteristics of Yunnan-produced cigar tobacco leaves, seven fermentation substrates, including formic acid chelates, acetic acid chelates, citric acid chelates, lactic acid chelates, acid salt chelates, sugar chelates, and distiller's lees supernatant, were selected as base media. Various combinations of these substrates were formulated to prepare compound fermentation media for the fermentation of the middle leaves of Yunxue No. 39 cigar filler tobacco. After fermentation, the conventional chemical constituents and volatile aroma compounds of the tobacco leaves were systematically analyzed. The results demonstrated that different fermentation media exerted distinct effects on the chemical composition and aroma quality of cigar tobacco leaves. Among the tested treatments, the B3 fermentation medium (acetic acid chelates combined with sugar chelates) markedly reduced the content of tobacco-specific nitrosamines (TSNAs). A2 fermentation medium (formic acid chelates combined with acid salt chelates) significantly increased chloroplast pigment content by 28.26%. Furthermore, D4 fermentation medium, consisting of lactic acid chelates, acid salt chelates, sugar chelates, and distiller's lees supernatant, produced the highest total volatile aroma content in the fermented cigar tobacco leaves, with the concentration of the characteristic aroma compound neophytadiene increasing from 615.12 μg/g to 1 236.24 μg/g. This study provided theoretical guidance and experimental evidence for improving cigar tobacco fermentation technology and developing novel fermentation media.
    The influence of inter-row grass mulching on the growth characteristics and orchard microenvironment of kiwifruit
    ZHAI Jing-hua, JI Xiao-mei, ZHANG Hong, CHEN Zhi-wei, YUE You-zhang, LI Xiu-li
    2026, 65(7):  123-126.  doi:10.14088/j.cnki.issn0439-8114.2026.07.019
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    To investigate the effects of inter-row grass mulching on the growth characteristics, photosynthetic characteristics and orchard microenvironment of kiwifruit(Actinidia chinensis Planch.), the 4-year-old kiwifruit Jinfeng Youxi was used as the research object. Three treatments were established including MSZ(inter-row planting of Vicia villosa Roth.),SMC(inter-row planting of Vulpia myuros), and CK(natural grass cover). The biological characteristics, photosynthetic characteristics, temperature and relative humidity of the orchard at 1 m above the orchard floor were measured. The results indicated that compared with CK, the MSZ and SMC treatments increased leaf length by 2.74% and 8.38%, leaf width by 1.39% and 2.02%, and hundred-leaf weight by 6.86% and 8.43%, respectively. The SMC treatment increased the current-year shoot diameter by 8.86%, while the leaf water content decreased by 1.80% and 0.12%, respectively, though the overall leaf water content remained within a relatively stable range. The MSZ treatment effectively increased the chlorophyll content of kiwifruit leaves by 9.17%. Both MSZ and SMC treatments reduced the total nitrogen, total phosphorus, and total potassium content in kiwifruit leaves, but the reduction was smaller under the SMC treatment. Both MSZ and SMC treatments effectively enhanced the net photosynthetic rate, transpiration rate and stomatal conductance, reduced intercellular CO2 concentration, and increased the instantaneous water use efficiency of kiwifruit leaves. Compared with CK, the MSZ and SMC treatments effectively reduced air temperature in kiwifruit orchards in August by 0.10 ℃ and 1.44 ℃, respectively, and increased the relative air humidity by 5.37 and 1.76 percentage points, respectively. Therefore, inter-row planting of Vicia villosa or Vulpia myuros in kiwifruit orchards was an effective agricultural management practice and worth promoting.
    Medicinal Plant
    Effects of different nutrient solution formulations on the growth and quality of Dendrobium nobile seedlings
    SHEN Zhong-guo, LYU Chao-yan, SUI Chang-ling, QIAN Zheng-min, HAN Chang
    2026, 65(7):  127-132.  doi:10.14088/j.cnki.issn0439-8114.2026.07.020
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    To clarify the effects of different nutrient solution formulations on the growth and quality of Dendrobium nobile, six different nutrient solution treatments were applied to Dendrobium nobile seedlings. Growth indicators including survival rate, number of new shoots, stem length, stem diameter, internode length, leaf length, leaf width, and fresh stem weight, as well as quality indicators including soluble sugar content, soluble protein content, and free amino acid content, were measured. The results showed that compared with the control (CK), no significant differences in seedling survival rate were observed among the nutrient solution treatments. T2 demonstrated a clear advantage in promoting the number of new shoots of Dendrobium nobile. The stem length, internode length, leaf length, leaf width, and fresh stem weight of T2 were relatively higher than those of CK and the other treatments, indicating relatively better performance in seedling growth. T1, T2, and T5 exhibited relatively high levels of soluble protein content, soluble sugar content, and free amino acid content, showing relatively good performance in seedling quality. Subordinate function analysis showed that T2 achieved the highest comprehensive score of 0.72. Overall, T2 nutrient solution performed relatively well in both the growth and quality of Dendrobium nobile seedlings.
    Combined flow cytometry and K-mer analysis for genome size estimation and genomic characterization of Schnabelia terniflora
    DENG Yu-jie, ZHANG Ze-zhi, YOU Lei, ZHANG Yong-hong, ZHENG Lan-lan
    2026, 65(7):  133-139.  doi:10.14088/j.cnki.issn0439-8114.2026.07.021
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    To further elucidate the genetic background of Schnabelia terniflora and promote the development of its genomic resources, flow cytometry was used to estimate the genome size of Schnabelia terniflora, with maize used as the reference plant. Meanwhile, the genome of Schnabelia terniflora was sequenced using the Illumina sequencing platform, and K-mer analysis was used to analyze the heterozygosity, GC content, and other genetic information of the Schnabelia terniflora genome, followed by NT alignment. The results showed that the genome size of Schnabelia terniflora estimated by flow cytometry was approximately 1 210 Mb. A total of 103.77 Gb of high-quality data was obtained using the Illumina sequencing platform, with an average sequencing depth of 73×. The genome size of Schnabelia terniflora was estimated to be approximately 1 220 Mb, with a heterozygosity rate of 1.18%, a GC content of 38.04%, and a repetitive sequence proportion of 80.20%. A comparison revealed that the genome size estimated by K-mer analysis was basically consistent with the results detected by flow cytometry. In addition, according to the NT database alignment results, among all species with known sequences, the sequencing reads of Schnabelia terniflora showed the highest matching rate with Scutellaria galericulata (17.82%), followed by Schnabelia terniflora itself (10.44%), Ballota nigra (4.77%), Schnabelia oligophylla (3.24%), and other plants. The genome of Schnabelia terniflora exhibited relatively low heterozygosity and a high proportion of repetitive sequences.
    Effects and mechanisms of biological synergistic improvement on alleviating continuous cropping obstacles of under-forest Codonopsis pilosula
    YANG Yong, LIU Hai-xia, LI Ya-ni, WANG Yu-long, GUO Cun-wu, YE Chen, LIU Fei
    2026, 65(7):  140-144.  doi:10.14088/j.cnki.issn0439-8114.2026.07.022
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    To investigate the regulatory effects and mechanisms of biological synergistic improvement on continuous cropping obstacles of under-forest Codonopsis pilosula, two-year continuously cropped Codonopsis pilosula under pear forest in Pingshun County, Shanxi Province was used as the test material, and five treatments including a water control, single biological amendments and compound biological amendments were set up. Plant growth, physiological resistance, active ingredient content, soil physicochemical properties, rhizosphere microorganisms, and the occurrence of root rot were measured. The results showed that the treatment of 1 235.5 kg/hm2 biological organic fertilizer + 24.71 kg/hm2 biological soil amendment had the best effect. Compared with the control, this treatment significantly increased plant height, stem diameter, and main root length by 34.4%, 41.2%, and 28.4%, respectively, and increased fresh weight per root and dry weight per root by 52.3% and 55.6%, respectively. Leaf peroxidase (POD) activity increased by 141.2%, malondialdehyde(MDA) content decreased by 42.08%, and soluble protein content increased by 96.62%. The contents of lobetyolin and polysaccharide in laternal roots increased by 61.33% and 27.35%, respectively. Soil pH was adjusted to 6.5, organic matter content increased by 135.29%, the number of rhizosphere bacteria increased significantly, while the numbers of fungi and Fusarium decreased significantly. The incidence of root rot decreased to 6.67%, with a control effect of 84.3%. Biological synergistic improvement could effectively alleviate continuous cropping obstacles of under-forest Codonopsis pilosula by optimizing the soil microecology, enhancing plant physiological resistance, and promoting the accumulation of active ingredients.
    Animal Science
    Effects of Houttuynia cordata on immunity of Pelophylax nigromaculatus against Elizabethkingia miricola
    HE Wan-wan, LI Long-ju, LIU Lu-yi, LI Yuan, ZHU Ze-liang, ZHENG Cheng-peng, DENG Ping, ZHANG Li-qiang
    2026, 65(7):  145-149.  doi:10.14088/j.cnki.issn0439-8114.2026.07.023
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    To investigate the effects of Houttuynia cordata Thunb. on the disease resistance of the black-spotted pond frog (Pelophylax nigromaculatus) against Elizabethkingia miricola infection, graded concentrations of H. cordata were supplemented into formulated feeds in the present trial. Blood biochemical and hepatic physiological parameters were determined, and the survival rate was recorded post artificial challenge with E. miricola. The results showed that after 9 weeks of dietary administration, dietary H. cordata significantly elevated serum lysozyme (LYS) activity as well as complement C3 and C4 contents in P. nigromaculatus. No significant variations were observed in hepatic alanine transaminase (ALT) and antioxidant capacity (AOC), whereas hepatic superoxide dismutase (SOD) activity was markedly reduced. All tested supplemental levels of H. cordata significantly improved the survival rate of frogs following E. miricola challenge. Relative to the blank control group, dietary supplementation with 1%, 3% and 5% H. cordata powder improved survival rates by 39.13, 28.42 and 28.42 percentage points, respectively; supplementation with 0.4% H. cordata crude extract increased survival by 14.13 percentage points. Collectively, dietary H. cordata effectively enhanced the innate immunity of P. nigromaculatus against E. miricola, with 1% H. cordata powder identified as the optimal dosage. These findings provided fundamental references for the prevention and control of Elizabethkingia infection in the culture of black-spotted pond frogs.
    Establishment of mouse hyperuricemia model and detection method of serum uric acid in vivo
    YANG Jing-li, RAN Ya-lan, AI Lun-qiang, LIAO Lu-jing, TAN Yan-hong, LUO Jian-qun, WANG Qing-fang, LI Yu, HE Mei-jun
    2026, 65(7):  150-156.  doi:10.14088/j.cnki.issn0439-8114.2026.07.024
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    To establish a stable mouse model of hyperuricemia (HUA), develop a method for in vivo blood uric acid (UA) detection, and evaluate the effects of different modeling approaches on UA metabolism and liver and kidney tissues in mice, healthy male Kunming mice were used to compare various drug administration regimens and determine the optimal HUA modeling method. Serum UA levels were measured using minimally invasive blood sampling, and the analytical method was validated. Histopathological changes in the liver and kidney were assessed by hematoxylin and eosin (HE) staining, and the transcriptional expression levels of URAT1 and OAT1 in renal tissues were examined by RT-qPCR. Results showed that the optimal HUA modeling method was intragastric administration of a yeast extract‑hypoxanthine suspension combined with intraperitoneal injection of potassium oxonate (YE-HX). This method resulted in the highest serum UA level (276.76 μmol/L), which was 1.62-fold higher than that in the control group, and induced typical HUA‑associated inflammatory pathologies in the liver and kidney, without significantly affecting the transcriptional expression of URAT1 or OAT1 in the kidney. A novel in vivo UA detection method was established using minimally invasive tail‑tip blood sampling (15-20 μL). The method achieved a limit of detection of 0.92 μmol/L, a limit of quantification of 1.70 μmol/L, recovery rates ranging from 88.62% to 105.55%, and a relative standard deviation of 9.31%.
    Study on gonadal development of Gymnodiptychus pachycheilus
    LIU Xun, ZHANG Huan, ZHAO Xian-wen, LU Hong-ke, LU Xing-yu, CHEN Kai, LIU Yong, LIU Mao-chun
    2026, 65(7):  157-161.  doi:10.14088/j.cnki.issn0439-8114.2026.07.025
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    Aiming to systematically analyze the gonadal development and fecundity characteristics of Gymnodiptychus pachycheilus, and their relationships with environmental factors in this fish species, sampling was conducted in the mainstem and tributaries of the upper Yellow River in Qinghai Province from May to June and October to November during 2023-2025. During the domestication period, dead specimens were used for sex identification. The gonadal developmental stages were determined by visual inspection. The gonadosomatic index (GSI) was calculated using the gravimetric method, and absolute fecundity (F) and relative fecundity (FL) were determined. The correlations between these reproductive parameters and basic biological indicators were analyzed. Results showed that the gonadal development of G.pachycheilus could be divided into six stages, and the main spawning period was concentrated from May to June. The body length of sexually mature females ranged from 263 to 380 mm. The absolute fecundity was 7 592-14 047 eggs, and the relative fecundity was 61-90 eggs per gram. The peak gonadosomatic index (GSI) of males during the spawning season reached 5.55%. The egg diameter distribution indicated that the oocyte development followed a group-synchronous maturation pattern. Fecundity showed a significantly positive correlation with body length and body weight (P<0.05). Gymnodiptychus pachycheilus exhibited moderate fecundity and a group-syhchronous spawning pattern.
    Storage & Processing
    Optimization of extraction of polyphenols from grapevine (Vitis vinifera L.) leaf and their in vitro antioxidant and hypoglycemic activities
    XIAO Shang-yue, ZHAO Jia, FU Yu-liang, MAN Zheng-yin, SHI Jia-wei
    2026, 65(7):  162-170.  doi:10.14088/j.cnki.issn0439-8114.2026.07.026
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    This study aimed to establish and optimize an ultrasound-assisted ethanol extraction process for grapevine (Vitis vinifera L.) leaf, and to evaluate the in vitro antioxidant and hypoglycemic activities of the purified polyphenol fractions. Taking grapevine leaf as the material, the ultrasonic-assisted extraction method was applied to extract polyphenols. Single-factor experiments combined with a Box-Behnken response surface methodology were employed to optimize the extraction parameters and evaluate their effects on polyphenol yield. The results showed that the optimal extraction technology was as follows: ultrasonic temperature of 69 °C, ultrasonic time of 51 min, ethanol volume fraction of 61%, and liquid to material ratio of 40∶1 (mL∶g). The yield of polyphenols in the crude grapevine leaf extract reached (36.56±0.86) mg/g. After purification with macroporous resin, grapevine leaf polyphenols exhibited strong antioxidant activity in vitro, with IC50 values for DPPH and ABTS+ radical scavenging of 0.037 3 mg/mL and 0.091 3 mg/mL, respectively. Meanwhile, the grapevine leaf polyphenols showed significant inhibitory effects against α-amylase and α-glucosidase, with IC50 values of 0.076 3 mg/mL and 0.147 0 mg/mL, respectively. These results indicated that grapevine leaf polyphenols possessed strong antioxidant and hypoglycemic potential, providing a reference for the resource utilization of grapevine leaf agricultural by-products.
    Detection & Analysis
    Determination of the residue of abamectin B1a in rice by QuEChERS-UPLC-MS/MS
    LYU Ang, ZHANG Kai, ZHU Kun-miao, LIU Jun, CHEN Xin
    2026, 65(7):  171-177.  doi:10.14088/j.cnki.issn0439-8114.2026.07.027
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    Using the QuEChERS and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)technology, a detection method of abamectin B1a residues in various rice matrices was established. The results showed that the method exhibited good linearity in the concentration range of 0.005-0.500 mg/L (r≥0.995). The limits of quantification (LOQ) in brown rice, rice husk and straw were 0.01, 0.01 and 0.02 mg/kg, respectively. The recoveries ranged from 71% to 92%, with relative standard deviations of 3%-11%, meeting the requirements for residue analysis. Field trials indicated that abamectin degraded rapidly in rice. The half-lives in rice husk and paddy rice at the Hainan experimental site were 1.2 d and 1.7 d, respectively. At the final residue stage, abamectin residues in brown rice were all below the LOQ. Only a small amount of residues was detected in rice husk and straw at the Hainan site, and the residue level in rice husk was higher than that in brown rice, suggesting that abamectin mainly accumulated in rice husk. Rice dehulling could effectively reduce abamectin residues. The established method was efficient and reliable, and could provide technical support for the rational application of abamectin on rice and the supervision of agricultural product quality and safety.
    Agricultural Engineering
    Application of quality control technology for surface meteorological observation data in radish production in Dongying
    LIANG Hai-xia, LIANG Qian, ZHANG Li-qing
    2026, 65(7):  178-183.  doi:10.14088/j.cnki.issn0439-8114.2026.07.028
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    To enhance the application value of ground meteorological observation data in radish production in Dongying, this study focused on the meteorological sensitive indicators during the growth period of Dongying green radish. A meteorological data quality control technology system and a three-level quality control process adapted to radish production requirements were constructed, and an INLM-PSO missing value repair method was proposed. The results showed that the error rejection rate of the RF anomaly identification method ranged from 1.37% to 1.62% across different growth periods. The INLM-PSO missing value repair method achieved repair completeness rates over 98% at daily, weekly, and ten-day-period scales. In the application evaluation of radish production, the emergence rate during the sowing and emergence period remained above 86%, the rotten seed rate was controlled within 3% to 8%, and the emergence uniformity ranged from 80% to 95%, indicating better overall performance than the KNN neighborhood discrimination method and the improved 3σ threshold method. This meteorological data quality control system could reduce the false rejection and missed detection rates of anomalous data, improve the repair completeness rates of missing data, and provide reliable data support for radish production and field management decisions in Dongying.
    A multi-factor cotton futures price prediction method based on MVMD-BO-BiMamba
    CHEN Cong, ZHU Jing, YUAN Meng-yu, WANG Shuo-chao
    2026, 65(7):  184-193.  doi:10.14088/j.cnki.issn0439-8114.2026.07.029
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    Aiming at the prediction difficulties caused by the nonlinear and non-stationary characteristics of cotton futures prices and multi-factor coupling, a prediction model integrating multivariate variational mode decomposition (MVMD), Bayesian optimization (BO), and bidirectional Mamba was proposed, with the cotton futures settlement price as the prediction target variable. First, the orthogonal projection maximum information coefficient was used to screen key features; MVMD was adopted to jointly decompose the multi-dimensional feature sequences, extracting collaborative features and suppressing non-stationary noise. Then, the hyperparameters of the bidirectional Mamba model were optimized by the BO algorithm to avoid the blindness of manual parameter tuning.Finally, the optimized model was applied to the bidirectional temporal dependency prediction of each component. The results showed that in the multi-factor scenario, the comparative experiment indicated that the root mean square error (RMSE), mean absolute error (MAE), and mean absolute percentage error (MAPE) of the model were reduced to 58.05 yuan/t, 46.04 yuan/t, and 0.34%, respectively, and the coefficient of determination (R2) increased to 0.978, with prediction accuracy superior to that of the MVMD-BiMamba, BO-BiMamba, BiMamba, VMD-BO-BiMamba, Mamba, Transformer, and LSTM benchmark models.
    YOLOv12-based framework for plant disease detection enhanced by multi-scale feedback aggregation
    CHEN Ling, GAN Lu, CHEN Jia-ying, CHEN Jian-feng, DAI Qu-shun
    2026, 65(7):  194-200.  doi:10.14088/j.cnki.issn0439-8114.2026.07.030
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    To address the problems of difficult small-object recognition, insufficient feature fusion, and complex background interference in plant disease detection, a multi-scale feedback aggregation detection model based on improved YOLOv12 was proposed. The model was validated on the PlantDoc dataset containing images of 30 categories of plant leaf diseases to improve the detection accuracy and robustness of lesion regions in complex environments. By introducing the Multi-Scale Enhanced Multi-Branch Aggregation with Feedback(MEMBA-F) module into the YOLOv12 framework, the improved model employed multi-branch convolution and a bidirectional information feedback mechanism to achieve adaptive cross-layer feature fusion, thereby enhancing the representation of lesion edges and fine-grained features. The results showed that the Precision, Recall, mAP50, and mAP50-95 of the improved model on the PlantDoc dataset reached 0.573, 0.372, 0.582, and 0.365, respectively, which were 7.7%, 1.4%, 3.7%, and 7.7% higher than those of YOLOv8, while maintaining an inference speed of 27.4 ms, demonstrating real-time detection capability. Compared with the baseline YOLOv12 model, the metrics of the improved model were further enhanced, verifying the effectiveness of the MEMBA-F module in multi-scale feature aggregation, cross-layer information interaction, and the representation of small objects and fine-grained features. The improved model balanced detection accuracy and efficiency, providing a reference for plant disease recognition in smart agriculture.
    Design and performance test of a self-propelled vegetable transplanter for solar greenhouses in Xinjiang
    ZHENG Xue-ning, LIU Chen, DONG Zhi-ming, HE Yi-chang
    2026, 65(7):  201-207.  doi:10.14088/j.cnki.issn0439-8114.2026.07.031
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    A self-propelled vegetable transplanter was designed to address the technical challenges of low mechanization level, high labor cost, poor adaptability of existing equipment, and wheel slippage on sandy loam soil in vegetable transplanting for solar greenhouses in Xinjiang. The machine was developed based on the local agronomic pattern characterized by “narrow passages, sandy loam soil, and double-row planting”. The transplanter mainly consisted of a seedling supply and delivery mechanism, a transplanting mechanism, a hydraulic balance system, and a power transmission system, and its key structural parameters were determined through theoretical analysis. Field tests were conducted at the protected agriculture base in Ying'awati Township, Hotan Prefecture, Xinjiang. The results showed that the operating speed of the machine ranged from 1.0 to 2.0 km/h, and the transfer speed reached 4.0 to 6.0 km/h. Under the conditions of a plant spacing of 300 mm and a transplanting depth of 60 mm, the qualified upright seedling rate reached 96.9%, the lodging rate was 3.1%, and the plant spacing variation coefficient was 4.5%. All these indicators were superior to those of traditional manual transplanting, and the row spacing deviation was less than or equal to 3 mm. All indicators met the requirements of the industry standard JB/T 10291-2013 Dryland Planting Machinery and the agronomic requirements for mechanized vegetable transplanting in solar greenhouses in Xinjiang. This design improved the mechanization level and operational efficiency of protected agriculture transplanting.
    Estimation of tobacco LAI across multiple growth stages based on improved particle swarm optimization
    DENG Zhe-hong, WANG Wei, DENG Shi-feng, CUI Guo-xian, SHE Wei, WANG Shuai-bin, WANG Dong, CAO Xiao-lan
    2026, 65(7):  208-214.  doi:10.14088/j.cnki.issn0439-8114.2026.07.032
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    To address the issues of low efficiency in the traditional measurement of tobacco leaf area index (LAI), feature redundancy in remote sensing inversion, and the tendency of model parameter optimization to easily fall into local optima, tobacco LAI estimation models suitable for different growth stages were constructed. The rosette stage and vigorous growth stage of tobacco were taken as the target growth stages, and 25 remote sensing variables were extracted based on unmanned aerial vehicle (UAV) multispectral imagery. Feature selection was conducted using competitive adaptive reweighted sampling (CARS), least angle regression (LARS), and iteratively retains informative variables (IRIV) algorithms. The results demonstrated that the IRIV algorithm exhibited superior comprehensive performance and could effectively reduce prediction errors. Based on the optimally selected features, Random Forest (RF), XGBoost, Back Propagation (BP) neural network, and Support Vector Regression (SVR) models were established, among which the SVR model demonstrated the best baseline predictive capability. Furthermore, a Particle Swarm Optimization algorithm improved by the Tent chaotic map (TPSO) was introduced to optimize the parameters of the SVR model, thereby constructing the TPSO-SVR model. Compared with the PSO-SVR model, the coefficient of determination (R2) of the TPSO-SVR model on the test set at the rosette stage increased by 0.015 to 0.757, and the root mean square error (RMSE) decreased by 0.006 to 0.243; at the vigorous growth stage, the R2 on the test set increased by 0.021 to 0.872, and the RMSE decreased by 0.020 to 0.253. The results indicated that the stage-specific inversion method based on IRIV feature selection and the TPSO-SVR model could improve the estimation accuracy of tobacco LAI at key growth stages, providing a technical reference for the non-destructive monitoring of tobacco growth and precision field management.
    Biological Engineering
    Identification of the EIN3/EIL gene family in foxtail millet and its expression analysis in multiple tissues
    LIU Peng-cheng, WANG Ya-yu, YANG Tian-tian
    2026, 65(7):  215-220.  doi:10.14088/j.cnki.issn0439-8114.2026.07.033
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    In order to systematically elucidate the evolutionary characteristics, structural features, and potential regulatory functions of the EIN3/EIL (SiEIN3/EIL) gene family in foxtail millet (Setaria italica) during growth, development, and environmental adaptation, a systematic identification and multi-tissue expression profile analysis were conducted based on the whole-genome data of foxtail millet. Through combined HMMER and BLASTP searches, a total of four EIN3/EIL family members were identified in foxtail millet, with gene IDs of Seita.1G205600.1, Seita.7G121700.1, Seita.2G437500.1, and Seita.9G419900.1. Bioinformatics analysis indicated that all proteins of this family were localized in the nucleus and were hydrophilic, distributed dispersedly across four chromosomes; all members contained exons, shared six core conserved motifs, and their promoter regions were enriched with cis-acting elements associated with light responses, multiple phytohormone responses (e.g., methyl jasmonate, abscisic acid), and abiotic stress responses. Expression analysis of multi-tissue transcriptome data revealed differences in the expression levels among the genes, among which Seita.9G419900.1 exhibited significantly higher expression from the jointing stage to the flowering stage than during vegetative growth stages such as the seedling stage, playing a core regulatory role mainly in reproductive development and floral organ formation.
    Economy & Management
    Impact of building geographical indication brands for agricultural products on high-quality agricultural development amid industrial restructuring
    LI Jia-lin, XU Qing-wei, LI Bing-jun
    2026, 65(7):  221-232.  doi:10.14088/j.cnki.issn0439-8114.2026.07.034
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    To explore the impact of building agricultural product geographical indication brands on high-quality agricultural development and the threshold effects of industrial structure, using panel data from 30 provinces in China from 2008 to 2023, an evaluation system comprising five dimensions—innovation, coordination, green development, openness, and sharing—was constructed and the entropy method was employed to objectively measure the level of high-quality agricultural development across provinces. Based on this, the two-way fixed effects model, instrumental variables, and panel threshold model were employed to empirically test the direct impact of agricultural product geographical indication brand development on high-quality agricultural development, its heterogeneity characteristics, and the moderating role of industrial structure. The results showed that agricultural product geographical indication brand development had a significant positive impact on high-quality agricultural development, primarily manifested in the innovation and sharing dimensions; however, this impact exhibited significant spatial heterogeneity, exerting a significant driving effect only in the central region. This promotional effect was subject to a single-threshold constraint imposed by industrial structure. When the level of industrial structure had not exceeded the threshold value of 1.067, the brand′s driving effect was limited and failed to pass the significance test; once the threshold of 1.067 was crossed, supported by the development and complementary infrastructure of modern service industries, the brand empowerment effect had experienced a substantial leap and was highly significant at the 1% statistical level, exhibiting a distinct nonlinear characteristic of increasing marginal utility.
    Reconstruction and empirical analysis of the evaluation indicator system for agricultural modernization during the process of digital transformation: Taking 153 villages in Yubei District, Chongqing as an example
    ZHOU Lin, WU Zhao-juan, CHEN Wen-yan, LIU Peng
    2026, 65(7):  233-240.  doi:10.14088/j.cnki.issn0439-8114.2026.07.035
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    To scientifically evaluate the level of agricultural modernization in the process of digital transformation, from a micro-perspective of villages and based on the theories of agricultural modernization and digital agriculture, a comprehensive evaluation system for agricultural modernization was reconstructed. The system covered 5 dimensions and 17 specific indicators. Based on field surveys, primary village-level data were collected from 153 administrative villages in Yubei District, Chongqing. An empirical analysis was conducted using methods such as the Analytic Hierarchy Process (AHP), entropy weighting method, Moran′s Index, and obstacle degree model. The results indicated that the agricultural modernization index at the village level in Yubei District showed significant variation (0.001 2-0.261 4), with high-level and relatively high-level villages accounting for only 13.08%. The region was generally in the initial stage of digital transformation and exhibited a distinct positive skewness. In terms of spatial patterns, village-level agricultural modernization levels were characterized by a discrete and random distribution with weak inter-regional linkage, and a large-scale collaborative feature had yet to be formed. The output value of leisure agriculture, the application of intelligent equipment, and the proportion of online agricultural product sales were the primary obstacle factors affecting the level of agricultural modernization in Yubei District. It was recommended to further promote the development of agricultural modernization by advancing coordinated regional development, deepening the integrated application of science and technology, promoting the deep integration of primary, secondary, and tertiary industries, and strengthening the cultivation of digital talent.
    Rural Revitalization
    Research on the shaping and communication of characteristic agricultural product brand culture: A case study of Enshi potato
    TAN Xiao-hua
    2026, 65(7):  241-248.  doi:10.14088/j.cnki.issn0439-8114.2026.07.036
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    To enhance the quality and efficiency of regional characteristic agriculture and ensure sustained income growth for farmers, using Enshi potatoes as the research subject, the current achievements and multiple challenges in shaping the brand culture of Enshi potatoes within the context of rural revitalization were systematically examined. From four dimensions—subject empowerment, content narrative, comprehensive channel integration, and audience engagement—a systematic framework for advancing Enshi potato brand culture development was established to explore pathways for branding distinctive agricultural products. This initiative aimed to strengthen the core competitiveness of the Enshi potato brand and enhance the industry′s value while driving comprehensive rural revitalization through a unique brand culture.
    Research on the responsive collaborative empowerment mechanism for inter-village development:Empirical analysis based on the “1+7” inter-village shared prosperity model of Shibadong Village, Hunan Province
    LUO Si-yue
    2026, 65(7):  249-256.  doi:10.14088/j.cnki.issn0439-8114.2026.07.037
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    To optimize the effectiveness of regional collaborative governance in rural revitalization, the practice of inter-village shared prosperity model in Shibadong Village, Hunan Province was analyzed by employing literature review, case study, and questionnaire survey methods and innovatively applying the theory of responsive collaborative governance, to examine the strengths and weaknesses of the “1+7” inter-village shared prosperity model and to explore the optimization paths for responsive governance and collaborative governance. Empirical analysis revealed that the current model possessed synergistic advantages in industry, resources, and governance, but it also faced prominent contradictions in three areas: resources, policy, and culture. By optimizing the demand response mechanism of “mobilization-pain points-response”, grasping the element correlation and coordination of “context-participants- process”, guided by the dual approaches of digital empowerment and endogenous development, this initiative achieved the joint prosperity of the seven villages through value reconstruction, industrial restructuring, and governance upgrading, in order to provide practical insights for rural revitalization and sustainable development in similar impoverished areas.