Abstract: In order to obtain a large number of Bletilla striata seedlings,expand the cultivated area and realize the large-scale field production,L₉（3³） orthogonal experiment was conducted to study the environmental conditions of B. Striata seeds.The results showed that,the combination of substrate with seedling substrate∶matured organic matter=1∶1,temperature 25 ℃,light duration 16 h/d and humidity 100% was the most favorable for the germination of B. striata seeds,with the emergence rate reaching up to 69.45%. The impact degree of temperature was the biggest,followed by matrix ratio,and the third was illumination duration.

Key words: Bletilla striata, direct-sowing breeding seedling, environmental factors, orthogonal test