Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana

doi:10.14088/j.cnki.issn0439-8114.2018.02.029

HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (2): 115-118.doi: 10.14088/j.cnki.issn0439-8114.2018.02.029

• Biological Engineering • Previous Articles Next Articles

Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana

CHEN Da-qing¹, ZHONG Yu-hai², LI Ying-sheng, SU Ai-guo, ZHU Yun-fen⁴, LI Ya-nan

1.College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;
2.Enshi Seed Authority,Enshi 445000,Hubei,China;
3a.School of Life Science;3b.Development and Planning Department,Yangtze University,Jingzhou 434023,Hubei,China;
4.Enshi Institute of Selenium Application Technology and Product Development,Enshi 445000,Hubei,China

Received:2018-01-05 Online:2018-01-25 Published:2018-01-10

Abstract

Abstract: A selenate-torlerance arabidopsis mutant was obtained by using the root-bending trait under selenate stress. It was conformed that the selenate resistant characteristics could be steady inheritance. Then this mutant was crossed with the wild-type Ler. and F₁ generation were created with heterozygous type completely. The F₂ crossed by theirself were expressed the separation ratio of 3∶1 in response of sensitive selenium to resistent selenium,It were suggested that this mutant was a monogenic recessive mutation. Map-based cloning population were composed of the 132 selenate-tolerance seedlings in F₂ generation. Using SSLP and In/Del based on PCR to preliminary chromosomal location. Through linkage analysis,it's indicated that this mutant gene was located on the chromosome I,which was mapped between molecular marker F12P19 and NGA111.

Key words: Arabidopsis thaliana, selenate stress, mutant, gene lacation

CLC Number:

CHEN Da-qing, ZHONG Yu-hai, LI Ying-sheng, SU Ai-guo, ZHU Yun-fen, LI Ya-nan. Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(2): 115-118.

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Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana

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