Study on the response of Urechis unicinctus and amylase activity under Cd2+ stress

doi:10.14088/j.cnki.issn0439-8114.2020.18.012

Abstract

Abstract: Used hydrostatic test, cadmium ions solution （0, 0.2, 0.5, 0.8, 1.1 mg/L） prepared with deionized water at a water temperature of （10 ± 2） ℃ was used for 48 h acute toxicity test to Urechis unicinctus. The results showed that the activity of amylase in the body cavity of Urechis unicinctus was simply negatively correlated with the concentration of Cd²⁺ in the living water body of Urechis unicinctus, and with the increasing concentration of Cd²⁺ in the exposed water, Urechis unicinctus showed varying degrees of stress change.

Key words: cadmium, Urechis unicinctus, acute toxicity, amylase, biological indicator

CLC Number:

WANG Shu-yang, WANG Yue-chen, DU Xing-lin, MU Jin-hua, LYU Dong-wei. Study on the response of Urechis unicinctus and amylase activity under Cd²⁺ stress[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(18): 63-66.

References

[1] 杨金龙,吴晓郁,石国峰,等.海洋牧场技术的研究现状和发展趋势[J].中国渔业经济,2004(5):48-50.
[2] 贾晓平,蔡文贵,林钦.我国沿海水域的主要污染问题及其对海水增养殖的影响[J].中国水产科学,1997,4(4):78-82.
[3] 崔毅,陈碧鹃,宋云利.胶州湾海洋动物体中重金属含量及评价[J].海洋环境科学,1996(4):17-22.
[4] FAN D P,PAN L Q,XIAO G Q,et al.The effect of pH and temperature on digestive enzyme activity in Sinonoracula constricta[J].Transactions of oceanology and limnology,2003(4):69-73.
[5] SUPANNAPONG P,PIMSALEE T,A-KOMOL T,et al.Digestive enzymes and in-vitro digestibility of different species of phytoplankton for culture of the freshwater pearl mussel, Hyriopsis(Hyriopsis)bialatus[J].Aquaculture international,2008,16(5):437-453.
[6] HASSETT R P,LANDRY M R.Seasonal changes in feeding rate, digestive enzyme activity, and assimilation efficiency of Calanus pacificus[J].Limnology & oceanography,1990,62(3):203-210.
[7] 李俊辉,王庆恒,杜晓东,等.温度和pH 对马氏珠母贝肝胰脏消化酶活力的影响[J].水产科学,2011,30(2):115-117.
[8] COEN W M D,JANSSEN C R. The use of biomarkers in Daphnia magna toxicity testing II. Digestive enzyme activity in Daphnia magna exposed to sublethal concentrations of cadmium, chromium and mercury[J].Chemosphere,1997,35(5):1053-1067.
[9] 吴众望,潘鲁青,董双林.9种金属离子对缢蛏消化酶活力的影响[J].中国水产科学,2003,10(4):297-300.
[10] 辛碧芬,谢进金,庄巧阳.几种金属离子对鲢肝胰脏淀粉酶的影响[J].水生态学杂志,2009,2(3):91-95.
[11] LAI T H,HE B Y,FAN H Q,et al.Effects of cadmium stress on the activities of antioxidant enzymes,digestive enzymes and the membrane lipid peroxidation of themangrove mud clam Geloina coaxans(Gmelin)[J].Acta ecologica sinica,2011,31(11):3044-3053.
[12] CHEN Z,MAYER L M,WESTON D P,et al.Inhibition of digestive enzyme activities by copper in the guts of various marine benthic invertebrates[J].Environmental toxicology and chemistry,2002,21(6):1243-1248.
[13] 王晶. 沙蚕暴露于镉、铜和石油烃单一与复合污染的生态毒理学研究[D].哈尔滨:东北农业大学,2007.
[14] 唐永政,宋祥利,翟传阳,等.3种重金属离子对单环刺螠幼螠的急性毒性研究[J].烟台大学学报(自然科学与工程版),2017,30(1):31-35.
[15] 张晓举,赵升,冯春晖.渤海湾南部海域生物体内的重金属含量与富集因素[J].大连海洋大学学报,2014,29(3):267-271.
[16] 王惠艳,陈亮,胡树起.渤海湾西部海域表层沉积物重金属含量分布与评价[J].物探与化探,2016,40(3):609-613.
[17] 孙钦帮,王阳,张冲,等.渤海湾西南部近岸海域表层沉积物重金属的含量分布与污染评价[J].人民珠江, 2016,37(10):89-93.
[18] 陈岩. 渤海湾潮间带表层沉积物中重金属含量的空间分异[D].北京:中国科学院地理科学与资源研究所,2007.
[19] 陈肖肖,高业田,吴洪喜,等.重金属Cd²+和Cu²+胁迫下泥蚶消化酶活性的变化[J].生态学报,2013,33(24):7690-7698.
[20] 李金龙,秦贞奎,史晓丽,等.铜离子对单环刺螠的毒性及对体壁抗氧化酶活性的影响[J].海洋湖沼通报,2012(2):77-82.
[21] 洪法水,王雪峰,吴康,等.重金属离子对猪胰α-淀粉酶活性影响的作用机理研究[J].高等学校化学学报,2001,12(22):1979-1983.
[22] MARKERT B,WAPPELHORST O,WECKERT V,et al.The use of bio-indicators for monitoring the heavy metal status of the environment[J].Journal of radioanalytical & nuclear chemistry,1999,240(2):425-429.
[23] 马藏允,刘海,姚波,等.几种大型底栖生物对Cd,Zn,Cu的积累实验研究[J].中国环境科学,1997,17(2):151-155.

Study on the response of Urechis unicinctus and amylase activity under Cd²⁺ stress

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