Experimental research on the separation of L-serine by ion exchange method

doi:10.14088/j.cnki.issn0439-8114.2021.18.027

Abstract

Abstract: By studying the adsorption and desorption ability of four different kinds of ion exchange resins named 201×7, AB-8, D301R and DAION PA312 to L-serine, the better resin named DAION PA312 was selected, and the best adsorption and desorption process parameters were also determined. Adsorption conditionsThe sample solution pH=9, the adsorption time was 20min, the actual exchange capacity was 94.25 mg/g; Desorption conditionsEluent was 0.2mol/L of phosphate buffer solution （pH=5）, elution flow rate was 0.4 mL/min. The results showed that DAION PA312 had a high adsorption amount and lower adsorption time, which was suitable for the separation and purification of L-serine.

Key words: ion exchange resin, L-serine, adsorption, desorption, separation and purification

CLC Number:

O629.7

ZHENG Gui-hua, LI Shi-yao, ZHANG Ting, CUI Xiong, ZHANG Wen-hao, SUN Jing, QIU Cheng. Experimental research on the separation of L-serine by ion exchange method[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(18): 133-136.

References 14

[1]	徐虹,滕农,应汉杰,等.离子交换法提取L-苯丙氨酸的研究[J].离子交换与吸附, 1999(1): 81-85.
[2]	余炜,伍时华,王恒山,等.离子交换法分离提取发酵液中L-亮氨酸[J].化学世界,2005(2):109-113.
[3]	SIMON G, HANÁK L, SZÁNYA T, et al. Ion-exchange parametric pumping:A cleaner separation method for producing amino acids from waste of the leather industry[J]. Journal of cleaner production, 1998, 6(3): 329-334.
[4]	SIMON G, GREVILLOT G, HANÁK L, et al. Preparative scale separation of amino acids by using thermal ion exchange parametric pumping[J]. Chemical engineering science, 1997,52(4):467-480.
[5]	翁连进,甘林火,王士斌,等.大孔强酸型阳离子交换树脂对L-精氨酸的吸附[J].过程工程学报, 2004,4(5): 391-396.
[6]	张义萍,张伟国,郝刚.离子交换法从发酵液中提取L-精氨酸[J].食品与发酵工业,2005,31(9):63-65.
[7]	翁连进,甘林火,王士斌,等.717型阴离子交换树脂吸附L-赖氨酸的特性[J]. 离子交换与吸附, 2004,20(2):119-124.
[8]	刘清华,张伟国,钱和.离子交换法从发酵液中提取L-异亮氨酸[J].离子交换与吸附, 2003,19(5): 457-462.
[9]	刘芳,谢丽敏,沈忠耀.离子交换吸附L-色氨酸的研究[J].生物工程学报, 1996,12(4):499-502.
[10]	徐颖,高建群.离子交换法精制丙氨酸的研究[J].氨基酸和生物资源,1995,17(3): 43-46.
[11]	王惟桂. 发酵液中L-脯氨酸分离纯化工艺的研究[D].广州:华南理工大学, 2013.
[12]	翁连进,邹建辉,甘林火,等. 717离子交换树脂对L-组氨酸的吸附及应用[J]. 沈阳药科大学学报, 2005,22(3): 223-225.
[13]	沈金玉,江秦宇.离子交换法提取谷氨酸的研究[J].离子交换与吸附,1994(4): 300-305.
[14]	ZHENG G H, JIN W W, FAN P, et al.A novel method for detecting amino acids derivatized with phenylisothiocyanate by high-performance liquid chromatography-electrospray ionization mass spectrometry[J]. International journal of mass spectrometry, 2015, 392:1-6.

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