Experiment of swim bladder collagen-chitosan-alginate hydrogel on skin wound healing in mice

doi:10.14088/j.cnki.issn0439-8114.2022.10.022

Abstract

Abstract: The eel bladder collagen extracted by acetic acid was used as the main drug, combined with chitosan and sodium alginate. The hydrogel dressing was prepared by Ca²⁺ cross-linking method, and its structure was observed under the scanning electron microscope. Through the skin wound experimental in vivo, the control group was treated with 0.9% normal saline and the experimental group was treated with hydrogel dressing. Then, the healing of skin wound was observed at 3, 7 and 14 days. The wound healing rate was calculated, the antioxidant and inflammatory indicators in serum were detected, and the histological changes of skin were observed by hematoxylin-eosin staining. The results showed that hydrogel showed a polypore structure under the scanning electron microscope. The serum antioxidant capacity of the hydrogel group was higher than that of the control group, and the level of inflammatory factors was lower than that of control group. Hydrogel dressing can significantly promote wound healing, reduce wound redness and inflammation, and completely repair the skin with smaller scars.

Key words: eel swim bladder, collagen, hydrogel, mice, wound healing

CLC Number:

LI Hang-ting, JIN Ming-yue, LI Nuo-ying, MO Yi-wen, TIAN Jing, YANG Zui-su. Experiment of swim bladder collagen-chitosan-alginate hydrogel on skin wound healing in mice[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(10): 127-131.

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