Abstract: To study the effects of Phaeoporus obliquus polysaccharose to non-alcoholic fatty liver disease in model mice induced by high-fat-high-fructose diet. Optimizing the extraction of Phaeoporus obliquus polysaccharose by orthogonal experiments, and the polysaccharide was used in mice with high sugar and fat-induced non-alcoholic fatty liver. Results showed that compared with normal group, model group mice liver increased obviously, and liver index increased significantly （P<0.01）. Compared with the model group, the mice liver index significantly decreased by the extraction of polysaccharides of Phaeoporus obliquus. The livers had different degrees of improvement, and the fasting blood sugar levels decreased significantly（P<0.01）. The mice serum TC, LDL-C, HDL-C and ALT levels decreased significantly the in high dosage group （120 mg/kg）（P<0.01）, except TG. Pathological results showed that mice liver cell damage have significantly improved with Phaeoporus obliquus polysaccharose. The liver cells arranged closer, central vein situation has improved significantly in high dose group. Phaeoporus obliquus polysaccharose has therapeutic effect in non-alcoholic fatty liver disease in model mice, and its mechanism may be related to lower blood lipids.

Key words: Phaeoporus obliquus, polysaccharide, NAFLD, mice