Abstract: E Liangyou 100 is a new high-quality two-line hybrid mid-season indica combination bred jointly by Institute of Food Crops, Hubei Academy of Agricultural Sciences, Anhui Zhengfeng Agricultural Science and Technology Co., Ltd. and Hubei Jiafengyuan Seed Industry Co., Ltd. The combination was bred by using two-line male sterile line Enong 1S as the female parent and restorer line R100 as the male parent. The combination participated in the regional test of mid-season indica late-maturing group in the middle and lower reaches of the Yangtze River from 2018 to 2019 and the production test in 2019 organized by Huachuang National Rice Science and Enterprise Association, showing the characteristics of high yield, good grain quality and suitable maturity. It was approved by the National Crop Variety Approval Committee in November 2020 （National Crop Variety Approval Committee 20200203）. The breeding process, characteristics, resistance, grain quality and yield performance of the rice（Oryza sativa L.） variety were summarized, and the key points of cultivation and seed production technology were introduced to provide reference for the application and promotion of the variety.

Key words: two-line hybrid medium indica rice （Oryza sativa L.）, E Liangyou 100, breeding, application