Abstract: The effects of disinfection methods on explants of forest medicine Pithecellobium clypearia （Jack） Benth., different concentrations of different plant growth regulators and different tissue culture methods on rooting and survival of tissue culture seedlings of Pithecellobium clypearia were studied. The most suitable disinfection method for explants of Pithecellobium clypearia was first treated with 75.00% ethanol for 1 min, and then treated with 5.00% calcium hypochlorite solution for 25 min. The germination rate was 70.00%. The most suitable ratio of plant growth regulators for rooting was IBA 0.40 mg/L + NAA 0.10 mg/L. Under this concentration, the rooting rate and transplanting survival rate of traditional tissue culture were 70.30% and 92.20%, respectively. The rooting survival rate and transplanting survival rate of sugar-free tissue culture were 100.00% and 99.90%, respectively. The best planting substrate ratio was river sand + peat soil （1∶1） and loess + river sand + peat soil （1∶1∶1）. The rooting rate of sugar-free tissue culture was higher than that of traditional tissue culture, and the root system was more developed, which was more suitable for commercial production.

Key words: Pithecellobium clypearia （Jack） Benth., explants, sugar-free tissue culture, rooting technology