Comparative analysis of transcriptome of Ganoderma lucidum mycelium with different nuclear numbers

doi:10.14088/j.cnki.issn0439-8114.2023.12.035

Abstract

Abstract: Using the mycelium of wild Ganoderma lucidum [Ganoderma lucidum （Leyss. ex Fr.） Karst.]（D）, natural mononuclear Ganoderma lucidum （Mo）, and binuclear Ganoderma lucidum （Mu） as materials, high-throughput sequencing technology was used to screen differentially expressed genes, and GO analysis and KEGG enrichment analysis were conducted. Through differential gene analysis, 568 differentially expressed genes were screened. The GO analysis results showed that the differentially expressed genes between wild Ganoderma lucidum （D） and natural mononuclear Ganoderma lucidum （Mo） hyphae were mainly enriched in transcriptional regulation, DNA templating, tRNA aminoacylation, and histone methylation processes;the differentially expressed genes in the mycelium of wild Ganoderma lucidum （D） and binuclear Ganoderma lucidum （Mu） were mainly enriched in processes such as chromatin silencing, L-serine metabolism, and sulfur compound metabolism;the differentially expressed genes in the mycelium of natural mononuclear Ganoderma lucidum （Mo） and binuclear Ganoderma lucidum （Mu） were mainly enriched in processes such as chromatin silencing, DNA template transcription, and glutamate decomposition. The KEGG enrichment results indicated that there were significant differences in the metabolic pathways between natural mononuclear Ganoderma lucidum （Mo） and binuclear Ganoderma lucidum （Mu） mycelium, with differential genes mainly annotated into pathways such as peroxidase and vitamin metabolism.The differential genes between wild Ganoderma lucidum （D） and natural mononuclear Ganoderma lucidum （Mo） mycelium, as well as between wild Ganoderma lucidum （D） and binuclear Ganoderma lucidum （Mu） mycelium, did not show KEGG enrichment, indicating no significant differences in their metabolic pathways. A total of 12 genes with significant differences in expression levels were selected from 3 strains, and the main functions of the genes were predicted.Out of 12 genes, 5 genes could be matched to the GO library. The GO annotation results indicated that these 5 genes were mainly annotated in aspects such as oxidoreductase activity, metal ion binding, membrane regulation, and membrane composition.

Key words: Ganoderma lucidum [Ganoderma lucidum （Leyss. ex Fr.） Karst.], transcriptome, mycelial nucleus number

CLC Number:

S567.3

WANG Hong-li, YANG Yang, HU Xue-bo. Comparative analysis of transcriptome of Ganoderma lucidum mycelium with different nuclear numbers[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 200-205,235.

References

[1] 汪戎锦,张鸿旭,武家杰,等.不同栽培土壤种植灵芝中主要成分的比较分析[J].人参研究,2022,34(2):2-5.
[2] 马传贵,张志秀,闫梅霞,等.灵芝的活性成分及其抗肿瘤研究进展[J].食药用菌,2022,30(2):114-118.
[3] 陈嘉骏,王颖,桑婷婷,等.灵芝多糖在糖尿病及其并发症防治中的研究进展[J].中草药2022, 53(3):937-947.
[4] 屠均亮,徐军伟.灵芝CRZ1的亚细胞定位及其编码基因在子实体不同发育阶段的转录水平分析[J].食用菌学报,2021,28(1):1-9.
[5] 李杰,蔡嘉慧,王慧中,等.药用植物基因组测序及功能基因组学研究进展[J].杭州师范大学学报(自然科学版),2021,20(4):364-373,397.
[6] 张闻婷,焦萌,王继华.药用植物基因组学研究进展[J].广东农业科学,2021,48(12):138-150.
[7] TONG T, YAN R, KANG J, et al.Chemical components of ganoderma[J]. Advances in experimental medicine and biology,2019,181:59-106.
[8] JIN M L,ZHANG H,WANG J J,et al.Response of intestinal metabolome to polysaccharides from mycelia of Ganoderma lucidum[J].International journal of biological macromolecules, 2019,122:723-731.
[9] 李希政,徐作成,夏宏志,等.灵芝菌株性状稳定性试验和品比试验[J].农业与技术,2022,42(4):14-16.
[10] CHEN S L, XU J, LIU C, et al.Genome sequence of the model medicinal mushroom ganoderma lucidum[J]. Nature communications,2012,3:913.
[11] 叶丽云,鲁欣,林强,等.灵芝单、双核菌丝差异研究[J].食品工业科技,2016,37(20):211-215.
[12] 连瑞丽,李宇伟,李存法,等.灵芝单双核菌丝液体发酵产多糖量的比较研究[J].郑州牧业工程高等专科学校学报,2012,32(2):14-16.
[13] SOHRETOGLU D, HUANG S.Ganoderma lucidum polysaccharides as an anti-canceragent[J]. Anti-Cancer agents in medicinal chemistry, 2018,18(5):667-674.
[14] JIN X, RUIZ BEGUERIE J, SZE D M, et al.Ganoderma lucidum (Reishi mushroom) for cancer treatment[J]. Cochrane database of systematic reviews, 2016,4:7731.
[15] YIN Z, YANG B, REN H.Preventive and therapeutic effect of ganoderma (Lingzhi) on skin diseases and care[J]. Advances in experimental medicine and biology, 2019,182:311-321.
[16] 胡延如. 木材降解物及温度光照对灵芝液体发酵三萜类物质产量的影响[D].武汉:华中农业大学,2016.
[17] JIANG Z, ZHOU X, LI R, et al.Whole transcriptome analysis with sequencing: methods, challenges and potential solutions[J]. Cellular and molecular life sciences, 2015,72(18):3425-3439.
[18] HRDLICKOVA R, TOLOUE M, TIAN B.RNA-Seq methods for transcriptome analysis[J]. Wiley interdisciplinary reviews-rna, 2017,8(1):1364.
[19] SEKI M, OKA M, XU L, et al.Transcript identification through long-read sequencing[J]. Methods in molecular biology, 2021,284:531-541.
[20] ZOABI Y, SHOMRON N.Processing and analysis of RNA-seq data from public resources[J]. Methods in molecular biology,2021,243:81-94.