Effects of ratio variations of earthworm feces extract-BG11 medium on the population growth of Chlorella pyrenoidosa

doi:10.14088/j.cnki.issn0439-8114.2025.10.023

Abstract

Abstract: To investigate the utilization potential of earthworm feces extract in microalgal cultivation, this study constructed composite culture systems incorporating 11 gradient-diluted vermicompost leachate formulations with BG11 medium and deionized water, systematically analyzing the effects of varying ratios on the growth kinetics of Chlorella pyrenoidosa. The results revealed that C3 (earthworm feces extract∶water = 5∶5, V/V) demonstrated optimal industrial applicability within the multidimensional evaluation framework. While attaining a 91.40% reduction in unit production cost relative to BG11 controls, this formulation sustained superior production performance, evidenced by a biomass concentration of (1.340 ± 0.071)×10⁷ cells/mL, accompanied by a 48.77% enhancement in specific growth rate and 84.67% decrease in attenuation rate compared to BG11 controls. These data substantiated that the developed formulation constitutes a technically viable approach for low-cost Chlorella pyrenoidosa production.

Key words: earthworm feces extract, Chlorella pyrenoidosa, BG11, population growth

CLC Number:

Q958.8

ZHENG Rui-han, MA Ruo-bing, XIE Zi-yang, LI Li-qiong. Effects of ratio variations of earthworm feces extract-BG11 medium on the population growth of Chlorella pyrenoidosa[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 153-157.

References

[1] 王宝贝,蔡舒琳,李丽婷,等.小球藻在食品中的应用研究进展[J].食品工业科技,2017,38(17):341-346,352.
[2] 袁婷. 培养基对蛋白核小球藻生长及蛋白质积累的影响研究[D].南宁:广西大学. 2024.
[3] 谭彤彤,李登来,席贝,等.小球藻粉的营养特性及作为新型非粮蛋白源对水产养殖动物生长性能、消化代谢及免疫功能的影响[J].饲料工业, 2025(15):34-40.
[4] ASWATHY U, NIDHIN S, MUTHU A, et al.Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production[J]. Systems microbiology and biomanufacturing,2022,2(2):1-11.
[5] 韩桂鸥,李霞,刘洪庆,等.林下养殖蚯蚓对盐碱地土壤和苗木生长的影响[J].天津农业科学,2016,22(4):79-82.
[6] 李艺坚,谢学方,孔令泽,等.蚯蚓养殖对幼龄胶园土壤养分及橡胶树根系生长的影响[J].热带作物学报,2018,39(12):2331-2335.
[7] 吴东阳,吴家欢,李伟志,等. 蚓粪、猪粪配施化肥对土壤质量、辣椒生长及品质的影响[J].生态环境学报,2024,33(9):1416-1425.
[8] 丁龙飞,张丽琼,朱文东,等. 蚯蚓养殖与蚯蚓粪有机肥的制作[J].中南农业科技,2024,45(1):95-98.
[9] 段睿洁,武玲,张航瑜,等. 蚯蚓液体肥料中细菌的分离鉴定及多样性研究[J].扬州大学学报(农业与生命科学版),2023,44(1):61-67,74.
[10] 邢益钊. 浅谈蚯蚓对土壤环境的指示作用及其功能的影响[J].热带农业科学,2020,40(12):87-90.
[11] 王伟. 土壤及其浸出液与藻类的适生性研究[D].重庆:西南大学,2011.
[12] 叶永茂,葛庆丰,吴满刚,等.基于OPLS-DA和熵权-Topsis法研究一氧化氮提升冷却猪肉品质[J].食品科学,2025(16):333-347.
[13] 梁颖,冯俊丽,徐方娇,等.一株产油微藻——小球藻的纯化鉴定与培养基的筛选[J].科技通报,2013,29(3):40-46.
[14] 王斌,李根,刘满强,等.不同生活型蚯蚓蚓粪化学组成及其性状的研究[J].土壤,2013,45(2):1313-1318.
[15] 曹月琴. 乙酸盐协同植物激素调控普通小球藻生长和代谢的机理研究[D].兰州:西北师范大学,2022.
[16] 李万年,余祥勇,张振波. 不同氮、磷浓度对4种饵料微藻生长的影响[J].水产养殖,2025,46(1):29-34.
[17] 阮铃铃. 植物多酚抑藻效能与其作用下藻细胞生理特征的研究[D].北京:北京林业大学,2011.
[18] 罗静波,陈树睿.EM菌发酵兔粪浸出液对小球藻生长及生化成分的影响[J].长江大学学报(自然科学版),2024,21(4):120-125.
[19] 简启刚,廖前柳,费树站,等.几种畜禽粪便浸出液培养小球藻的比较研究[J].凯里学院学报,2015,33(6):74-76.
[20] 仪凡. 典型微藻高浓度扩培及综合资源化效能研究[D].山东青岛:青岛理工大学,2024.
[21] 何雨晴,王红霞,胡强,等.肝素钠生产废水对小球藻生长和营养物去除的影响[J].食品与生物技术学报,2024,43(7):133-142.
[22] REVELLAME E D,AGUDA R,GATDUAL K M,et al.Microalgae in bioregenerative life support systems for space applications[J]. Algal research,2024,77(1):103332.