HUBEI AGRICULTURAL SCIENCES ›› 2025, Vol. 64 ›› Issue (8): 54-61.doi: 10.14088/j.cnki.issn0439-8114.2025.08.009

• Breeding & Cultivation • Previous Articles     Next Articles

The creation of waxy sorghum maintainer line resources by test cross identification combined with quantitative trait genetic analysis

HAN Yong-liang1, FANG Lu-bin1, LUO He-yue1, CUI Jiang-hui2, GUO Qun3, CHANG Jin-hua2   

  1. 1. Institute of Resources and Environment, Handan Academy of Agricultural Sciences, Handan 056000, Hebei, China;
    2. College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China;
    3. Hebei Hanqing Seed Technology Co., Ltd., Cangzhou 061000, Hebei, China
  • Received:2025-05-28 Online:2025-08-25 Published:2025-09-12

Abstract: The application of three-line hybrid waxy sorghum in production had been constrained by the scarcity of elite waxy maintainer germplasm resources. To rapidly create waxy sorghum maintainer germplasm resources, the F2 segregating population and F2:3 family population were constructed by using Aijiaonuo as the male parent and maintainer line TX623B as the female parent. The inheritance of sorghum quantitative traits such as plant height and stem diameter was analyzed by using the major gene+polygene mixed model. The results showed that the plant height was controlled by two pairs of major genes, and the heritability was high. Stem diameter was controlled by a pair of major genes, and the heritability was low. Based on the results of genetic analysis, a weighted comprehensive evaluation and screening system based on fuzzy similarity priority ratio was established by DPS data processing system, and the weighted comprehensive evaluation of F2:3 families was carried out. The application direction of excellent family materials in variety breeding was evaluated by comprehensive evaluation, and waxy maintainer materials with good comprehensive traits and phenotypic traits close to TX623B were screened out, which provided a method basis and material basis for sorghum variety breeding and heterosis utilization.

Key words: waxy sorghum, maintainer line, backcross breeding, test cross, quantitative trait, plant height, stem diameter, major gene + polygene mixed genetic model

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