Research progress on the application of RNA interference technology in the precise control of piercing-sucking pests

doi:10.14088/j.cnki.issn0439-8114.2026.03.015

Abstract

Abstract: In response to the challenges of growing insecticide resistance in piercing-sucking pests and the limitations of traditional chemical control methods, the research and application status of RNA interference (RNAi) technology in the green management of piercing-sucking pests was systematically reviewed. First, the mechanism of RNAi and its technical adaptability to the feeding behavior of piercing-sucking pests were elaborated. Next, key target genes validated in major pest groups, such as aphids, whiteflies, and planthoppers, as well as their interference effects, were summarized. The core bottlenecks hindering the industrialization of this technology were then analyzed, including issues such as dsRNA delivery efficiency, potential pest resistance, production costs, and environmental safety, along with corresponding optimization strategies. Finally, the application prospects of RNA-based pesticides and RNAi transgenic crops in future agricultural green pest management systems were discussed.

Key words: RNA interference, piercing-sucking pests, target gene(s), pest control, RNA-based pesticides

CLC Number:

CHENG Xi-yu, FANG Shang-ling, MIN Yong, LIU Xiao-yan, ZHU Lei, CHEN Ling. Research progress on the application of RNA interference technology in the precise control of piercing-sucking pests[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(3): 96-105.

References

[1] WARI D,KURAMITSU K,KAVALLIERATOS N G,et al.Sap-sucking pests; they do matter[J].Insects,2021,12(4):363.
[2] WILBY A,THOMAS M B.Natural enemy diversity and pest control: Patterns of pest emergence with agricultural intensification[J].Ecology letters,2002,5(3):353-360.
[3] MA C S,ZHANG W,PENG Y,et al.Climate warming promotes pesticide resistance through expanding overwintering range of a global pest[J].Nature communications,2021,12(1):5351.
[4] MARCHIORO C A,KRECHEMER F S.Potential global distribution of Diabrotica species and the risks for agricultural production[J].Pest management science,2018,74(9):2100-2109.
[5] BOSQUEE E,BOULLIS A,BERTAUX M,et al.Dispersion of Myzus persicae and transmission of potato virus Y under elevated CO₂ atmosphere[J].Entomologia experimentalis et applicata,2018, 166(5):380-385.
[6] HOGENHOUT S A,AMMAR E D,WHITFIELD A E,et al.Insect vector interactions with persistently transmitted viruses[J].Annual review of phytopathology,2008,46:327-359.
[7] BLACKMAN R L,EASTOP V F.Aphids on the world’s crops: An identification and information guide[M].Chichester, UK:John Wiley & Sons Ltd,1984.
[8] MORRISON W P,PEAIRS F B.Response model concept and economic impact[A].Response model for an introduced pest: The Russian wheat aphid[C].Lanham,M D,USA:Entomological society of America,1998:1.
[9] BRAR D S,VIRK P S,JENA K K,et al.Breeding for resistance to planthoppers in rice[A].HEONG K L,HARDY B. Planthoppers:New threats to the sustainability of intensive rice production systems in Asia[C]. Los Baños,Philippines:International rice research institute,2010:401-428.
[10] YANG L,ZHANG W L.Genetic and biochemical mechanisms of rice resistance to planthopper[J].Plant cell reports,2016, 35(8): 1559-1572.
[11] AKTER M,FAN L X,RAHMAN M M,et al.Vegetable farmers’ behaviour and knowledge related to pesticide use and related health problems: A case study from Bangladesh[J].Journal of cleaner production,2018,200:122-133.
[12] SHARAFI K,PIRSAHEB M,MALEKI S,et al.Knowledge, attitude and practices of farmers about pesticide use, risks, and wastes; A cross-sectional study (Kermanshah, Iran)[J].Science of the total environment,2018,645:509-517.
[13] TANG B,XU K K,LIU Y K,et al.A review of physiological resistance to insecticide stress in Nilaparvata lugens[J].3 Biotech,2022,12(3):84.
[14] ZHOU C S,LV H H,GUO X H,et al.Transcriptional analysis of Bemisia tabaci MEAM1 cryptic species under the selection pressure of neonicotinoids imidacloprid, acetamiprid and thiamethoxam[J].BMC genomics,2022,23(1):15.
[15] ZHANG R,YANG J,HU J Y,et al.Glutathione S-transferase directly metabolizes imidacloprid in the whitefly, Bemisia tabaci[J].Pesticide biochemistry and physiology,2024,201:105863.
[16] WEI Y Y,GUAN F,WANG R,et al.Amplicon sequencing detects mutations associated with pyrethroid resistance in Bemisia tabaci (Hemiptera: Aleyrodidae)[J].Pest management science,2021, 77(6):2914-2923.
[17] FELIX M,HOLST N,SHARP A.PestTox: An object oriented model for modeling fate and transport of pesticides in the environment and their effects on population dynamics of non-target organisms[J].Computers and electronics in agriculture,2019,166:105022.
[18] KARIMZADEH R,SCIARRETTA A.Spatial patchiness and association of pests and natural enemies in agro-ecosystems and their application in precision pest management: A review[J].Precision agriculture,2022,23(5):1836-1855.
[19] HANNON G J.RNA interference[J].Nature,2002,418(6894):244-251.
[20] JINEK M,DOUDNA J A.A three-dimensional view of the molecular machinery of RNA interference[J].Nature,2009,457(7228):405-412.
[21] TENLLADO F,DÍAZ-RUÍZ J R. Double-stranded RNA-mediated interference with plant virus infection[J].Journal of virology,2001,75(24):12288-12297.
[22] BARTEL D P. microRNAs: Genomics, biogenesis, mechanism, and function[J].Cell,2004,116(2):281-297.
[23] SIOMI M C,SATO K,PEZIC D,et al.PIWI-interacting small RNAs: The vanguard of genome defence[J].Nature reviews molecular cell biology,2011,12(4):246-258.
[24] RINN J L,CHANG H Y.Genome regulation by long noncoding RNAs[J].Annual review of biochemistry,2012,81: 145-166.
[25] WILL C L,LÜHRMANN R. Spliceosome structure and function[J].Cold spring harbor perspectives in biology,2011,3(7): a003707.
[26] KISS T, FILIPOWICZ W.Exonucleolytic processing of small nucleolar RNAs from pre-mRNA introns[J]. Genes & development,1995,9(11):1411-1424.
[27] CHEN L L.The biogenesis and emerging roles of circular RNAs[J].Nature reviews molecular cell biology, 2016,17(4):205-211.
[28] NAPOLI C,LEMIEUX C,JORGENSEN R.Introduction of a chimeric Chalcone Synthase gene into Petunia results in reversible co-suppression of homologous genes in trans[J].The plant cell,1990,2(4):279-289.
[29] FIRE A,XU S Q,MONTGOMERY M K,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature,1998,391(6669):806-811.
[30] MENDOZA-ALATORRE M,JULIAN-CHÁVEZ B,SOLANO-ORNELAS S,et al. RNAi in pest control: Critical factors affecting dsRNA efficacy[J].Insects,2025,16(7):737.
[31] HAUSSECKER D,KAY M A.Drugging RNAi[J].Science,2015,347(6226):1069-1070.
[32] LI Z,LI Q Y,PENG Q X,et al.RNAi of nuclear receptor E78 inhibits the cuticle formation in the molting process of spider mite, Tetranychus urticae[J].Pest management science,2025,81(2):809-818.
[33] MUTTI N S,LOUIS J,PAPPAN L K,et al.A protein from the salivary glands of the pea aphid, Acyrthosiphon pisum, is essential in feeding on a host plant[J].Proceedings of the national academy of sciences of the United States of America,2008,105(29):9965-9969.
[34] MAJIDIANI S,POURABAD R F,LAUDANI F,et al.RNAi in Tuta absoluta management: Effects of injection and root delivery of dsRNAs[J].Journal of pest science,2019,92(4):1409-1419.
[35] HAN S H,KIM J H,KIM K,et al.Selection of lethal genes for ingestion RNA interference against western flower Thrips, Frankliniella occidentalis, via leaf disc-mediated dsRNA delivery[J].Pesticide biochemistry and physiology,2019,161:47-53.
[36] TIMMONS L,COURT D L,FIRE A.Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans[J].Gene,2001,263(1-2):103-112.
[37] BETTENCOURT R,TERENIUS O,FAYE I.Hemolin gene silencing by ds-RNA injected into Cecropia pupae is lethal to next generation embryos[J].Insect molecular biology,2002,11(3):267-271.
[38] MUTTI N S,PARK Y,REESE J C,et al.RNAi knockdown of a salivary transcript leading to lethality in the pea aphid, Acyrthosiphon pisum[J].Journal of insect science,2006,6(38):1-7.
[39] SHARIF M N,IQBAL M S,ALAM R,et al.Silencing of multiple target genes via ingestion of dsRNA and PMRi affects development and survival in Helicoverpa armigera[J].Scientific reports,2022,12(1):10405.
[40] VOGEL E,SANTOS D,MINGELS L,et al.RNA interference in insects: Protecting beneficials and controlling pests[J].Frontiers in physiology,2019,9:1912.
[41] HERNÁNDEZ-SOTO A,CHACÓN-CERDAS R. RNAi crop protection advances[J].International journal of molecular sciences,2021,22(22):12148.
[42] SEPTIANI P,PRAMESTI Y,GHILDAN M,et al.RNAi-based biocontrol for crops: A revised expectation for a non-recent technology[J].Planta,2025,261(2):44.
[43] LI J Y,MENG S L,ZHANG Z X,et al.Nanoparticle-mediated calmodulin dsRNA and cyantraniliprole co-delivery system: High-efficient control of two key pear pests while ensuring safety for natural enemy insects[J].International journal of biological macromolecules,2024,277(Pt 3):134478.
[44] ZHENG H Y,HUA M K,JIANG M N,et al.Transgenic expression of mAChR-C dsRNA in maize confers efficient locust control[J].Plant communications,2025,6(5):101316.
[45] BAUM J A,BOGAERT T,CLINTON W,et al.Control of coleopteran insect pests through RNA interference[J].Nature biotechnology,2007,25(11):1322-1326.
[46] SINGH V,MANDHANIA S,PAL A,et al.Morpho-physiological and biochemical responses of cotton (Gossypium hirsutum L.) genotypes upon sucking insect-pest infestations[J].Physiology and molecular biology of plants,2022,28(11):2023-2039.
[47] WANG S F,GUO H J,ZHU-SALZMAN K,et al.PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection[J].Nature communications,2022,13:846.
[48] WU D W,QI T C,LI W X,et al.Viral effector protein manipulates host hormone signaling to attract insect vectors[J].Cell research,2017,27(3):402-415.
[49] ZHOU W,LI M M,ACHAL V.A comprehensive review on environmental and human health impacts of chemical pesticide usage[J].Emerging contaminants,2025,11(1):100410.
[50] WU M,DONG Y,ZHANG Q,et al.Efficient control of western flower thrips by plastid-mediated RNA interference[J].Proceedings of the National academy of sciences,2022,119(15):e2120081119.
[51] ZHANG Y X,YANG B J,YANG Z M,et al.Alternative splicing and expression reduction of P450 genes mediating the oxidation of chlorpyrifos revealed a novel resistance mechanism in Nilaparvata lugens[J]. Journal of agricultural and food chemistry,2023, 71(9):4036-4042.
[52] YANG C L,MENG J Y,ZHOU L,et al.Induced heat shock protein 70 confers biological tolerance in UV-B stress-adapted Myzus persicae (Hemiptera)[J].International journal of biological macromolecules,2022,220: 1146-1154.
[53] HAMDI F A,KATAOKA K,ARAI Y,et al.An octopamine receptor involved in feeding behavior of the two-spotted spider mite, Tetranychus urticae Koch: A possible candidate for RNAi-based pest control[J]. Entomologia generalis,2023,43(1):89-97.
[54] JI M Y,VANDENHOLE M,DE BEER B,et al.A nuclear receptor HR96-related gene underlies large trans-driven differences in detoxification gene expression in a generalist herbivore[J].Nature communications,2023,14(1):4990.
[55] XIA J,YANG Z,GONG C,et al.Genome-wide Identification and expression analysis of amino acid transporters in the whitefly, Bemisia tabaci (Gennadius)[J].International journal of biological sciences, 2017,13(6):735-747.
[55] XIA J X,YANG Z Z,GONG C,et al.Genome-wide identification and expression analysis of amino acid transporters in the whitefly, Bemisia tabaci (Gennadius)[J].International journal of biological sciences,2017, 13(6):735-747.
[56] HUANG M J,FU B L,YIN C,et al.Cytochrome P450 CYP6EM1 underpins dinotefuran resistance in the whitefly Bemisia tabaci[J].Journal of agricultural and food chemistry,2024,72(10):5153-5164.
[57] RAZA A,MALIK H J,SHAFIQ M,et al.RNA interference based approach to down regulate osmoregulators of whitefly (Bemisia tabaci): Potential technology for the control of whitefly[J].PLoS one,2016,11(4): e0153883.
[58] KIM Y H,SOUMAILA ISSA M,COOPER A M W,et al. RNA interference: Applications and advances in insect toxicology and insect pest management[J].Pesticide biochemistry and physiology,2015,120:109-117.
[59] TARIQ K,ALI A,EMYR DAVIES T G,et al. RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae[J].Scientific reports,2019,9: 5291.
[60] WANG H,DUAN W B,GAO R B,et al.Unique post-translational modifications diversify the sodium channels in peach aphid (Myzus persicae[Sulzer])[J].Pest management science,2021,77(10):4521-4529.
[61] WEI L Y,ZHANG L J,LIU N,et al.Effect of RNAi targeting CYP6CY3 on the growth, development and insecticide susceptibility of Aphis gossypii by using nanocarrier-based transdermal dsRNA delivery system[J].Pesticide biochemistry and physiology,2021,177:104878.
[62] XU L J,DUAN X L,LV Y H,et al.Silencing of an aphid carboxylesterase gene by use of plant-mediated RNAi impairs Sitobion avenae tolerance of phoxim insecticides[J].Transgenic research,2014,23(2): 389-396.
[63] GONG C,YANG Z Z,HU Y,et al.Silencing of the BtTPS genes by transgenic plant-mediated RNAi to control Bemisia tabaci MED[J].Pest management science,2022,78(3):1128-1137.
[64] MATSUMOTO Y,HATTORI M.The green rice leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), salivary protein NcSP75 is a key effector for successful phloem ingestion[J].PLoS one,2018,13(9): e0202492.
[65] DALAISÓN-FUENTES L I,PASCUAL A,GAZZA E,et al. Development of efficient RNAi methods in the corn leafhopper Dalbulus maidis, a promising application for pest control[J].Pest management science,2022, 78(7):3108-3116.
[66] YE C L,FENG Y L,YU F F,et al.RNAi-mediated silencing of the autophagy-related gene NlATG3 inhibits survival and fecundity of the brown planthopper, Nilaparvata lugens[J].Pest management science,2021, 77(10):4658-4668.
[67] YANG X B,ZHOU C,GONG M F,et al.Identification and RNAi-based functional analysis of four chitin deacetylase genes in Sogatella furcifera (Hemiptera: Delphacidae)[J].Journal of insect science,2021,21(4): 9.
[68] IBANEZ F,VIEIRA ROCHA S,DAWSON W O,et al.Gene silencing of cathepsins B and L using CTV-based, plant-mediated RNAi interferes with ovarial development in Asian Citrus psyllid (ACP), Diaphorina citri[J]. Frontiers in plant science,2023,14:1219319.
[69] PAREDES-MONTERO J R,ARIF U,BROWN J K. Knockdown of ecdysteroid synthesis genes results in impaired molting and high mortality in Bactericera cockerelli (Hemiptera: Triozidae)[J].Pest management science,2022,78(6):2204-2214.
[70] KHAN F,KIM M,KIM Y.Greenhouse test of spraying dsRNA to control the western flower Thrips, Frankliniella occidentalis, infesting hot peppers[J].BMC biotechnology,2023,23(1):10.
[71] WANG H Y,XIA B,WANG H F,et al.Fatty acid elongase gene PcELO7 is essential for lipid accumulation and fecundity of Panonychus citri (Acari: Tetranychidae)[J].Journal of agricultural and food chemistry, 2024,72(4):2100-2108.
[72] FINETTI L,PEZZI M,CIVOLANI S,et al. Characterization of Halyomorpha halys TAR1 reveals its involvement in (E)-2-decenal pheromone perception[J].The journal of experimental biology,2021,224(8): jeb238816.
[73] RIGA M,DENECKE S,LIVADARAS I,et al.Development of efficient RNAi in Nezara viridula for use in insecticide target discovery[J].Archives of insect biochemistry and physiology,2020, 103(3):e21650.
[74] LIU F Z,YANG B,ZHANG A H,et al.Plant-mediated RNAi for controlling Apolygus lucorum[J].Frontiers in plant science,2019,10:64.
[75] ZHANG X Y,ZHU X Q,GU S H,et al.Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals[J].Insect science, 2017,24(5):789-797.
[76] PARISE C,GALETTO L,ABBÀ S,et al.RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa[J].Scientific reports,2024,14:25812.
[77] DE SCHUTTER K,TANING C N T,VAN DAELE L,et al. RNAi-based biocontrol products: Market status, regulatory aspects, and risk assessment[J].Frontiers in insect science,2022,1:818037.
[78] JAIN R G,FLETCHER S J,MANZIE N,et al.Foliar application of clay-delivered RNA interference for whitefly control[J].Nature plants,2022,8(5):535-548.
[79] MAT JALALUDDIN N S,OTHMAN R Y,HARIKRISHNA J A. Global trends in research and commercialization of exogenous and endogenous RNAi technologies for crops[J].Critical reviews in biotechnology,2019,39(1):67-78.
[80] WHYARD S,SINGH A D,WONG S.Ingested double-stranded RNAs can act as species-specific insecticides[J].Insect biochemistry and molecular biology,2009,39(11):824-832.
[81] PRICE D R G,GATEHOUSE J A. RNAi-mediated crop protection against insects[J].Trends in biotechnology, 2008,26(7):393-400.
[82] MAO Y B,CAI W J,WANG J W,et al.Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol[J].Nature biotechnology,2007, 25(11):1307-1313.
[83] COLEMAN A D,WOUTERS R H M,MUGFORD S T,et al. Persistence and transgenerational effect of plant-mediated RNAi in aphids[J].Journal of experimental botany,2015,66(2):541-548.
[84] ABDELLATEF E,WILL T,KOCH A,et al.Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae[J].Plant biotechnology journal,2015, 13(6):849-857.
[85] SUN Y W,SPARKS C,JONES H,et al.Silencing an essential gene involved in infestation and digestion in grain aphid through plant-mediated RNA interference generates aphid-resistant wheat plants[J].Plant biotechnology journal,2019,17(5):852-854.
[86] WANG M,WEIBERG A,LIN F M,et al.Bidirectional cross-Kingdom RNAi and fungal uptake of external RNAs confer plant protection[J].Nature plants,2016,2:16151.
[87] WANG K X,PENG Y C,PU J,et al.Variation in RNAi efficacy among insect species is attributable to dsRNA degradation in vivo[J].Insect biochemistry and molecular biology,2016,77:1-9.
[88] COOPER A M,SILVER K,ZHANG J Z,et al.Molecular mechanisms influencing efficiency of RNA interference in insects[J].Pest management science,2019,75(1):18-28.
[89] QIAO H,ZHAO J,WANG X F,et al.An oral dsRNA delivery system based on chitosan induces G protein-coupled receptor kinase 2 gene silencing for Apolygus lucorum control[J].Pesticide biochemistry and physiology,2023,194:105481.
[90] YAN S,QIAN J,CAI C,et al.Spray method application of transdermal dsRNA delivery system for efficient gene silencing and pest control on soybean aphid Aphis glycines[J].Journal of pest science,2020,93(1): 449-459.
[91] MYSORE K,NJOROGE T M,STEWART A T M,et al. Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1 receptor genes[J].Scientific reports,2023,13:22511.
[92] HUANG Y J,HUANG J M,KIM D S,et al.The essential role of gut microbiota in dsRNA-mediated pest control of the phytophagous ladybird beetle, Henosepilachna vigintioctopunctata[J].npj biofilms and microbiomes,2025,11:141.
[93] LIU J Y,ZHANG L M,TONG H,et al.Microorganisms in mosquitoes for controlling mosquito-borne viral diseases: From lab to field[J/OL].Trends in microbiology,(2025-01-01)[2025-11-16]. https://doi.org/10.1016/j.tim.2025.01.001.
[94] SAJEESH S,LEE T Y,HONG S W,et al.Long dsRNA-mediated RNA interference and immunostimulation: A targeted delivery approach using polyethyleneimine based nano-carriers[J].Molecular pharmaceutics,2014, 11(3):872-884.
[95] MITTER N,WORRALL E A,ROBINSON K E,et al.Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses[J].Nature plants,2017,3:16207.
[96] MA Z Z,ZHENG Y,CHAO Z J,et al.Visualization of the process of a nanocarrier-mediated gene delivery: Stabilization, endocytosis and endosomal escape of genes for intracellular spreading[J].Journal of nanobiotechnology,2022,20(1):124.
[97] DONG Y,WU M T,ZHANG Q,et al.Control of a sap-sucking insect pest by plastid-mediated RNA interference[J].Molecular plant,2022,15(7):1176-1191.
[98] TIAN W,ZHANG T,ZHAO J H,et al.HIGS-mediated crop protection against cotton aphids[J].Plant biotechnology journal,2025,23(3):692-694.
[99] XU Q Q,SHANG F,FENG S Y,et al.Design the fusion double-strand RNAs to control two global sap-sucking pests[J].Pesticide biochemistry and physiology,2024,205:106114.
[100] HU B,XING Z P,DONG H,et al.Cytochrome P450 CYP6AE70 confers resistance to multiple insecticides in a lepidopteran pest, Spodoptera exigua[J].Journal of agricultural and food chemistry,2024,72(42): 23141-23150.
[101] ZHANG H H,ZOU J Z,YANG B J,et al.Importance of CYP6ER1 was different among neonicotinoids in their susceptibility in Nilaparvata lugens[J].Journal of agricultural and food chemistry,2023,71(9): 4163-4171.
[102] NI M,MA W,WANG X F,et al.Next-generation transgenic cotton: Pyramiding RNAi and Bt counters insect resistance[J].Plant biotechnology journal,2017,15(9):1204-1213.
[103] BHATIA V, BHATTACHARYA R.Host-mediated RNA interference targeting a cuticular protein gene impaired fecundity in the green peach aphid Myzus persicae[J].Pest management science,2018,74(9): 2059-2068.
[104] YANG L,TIAN Y,PENG Y Y,et al.Expression dynamics of core RNAi machinery genes in pea aphids upon exposure to artificially synthesized dsRNA and miRNAs[J].Insects,2020,11(2):227.
[105] CAGLIARI D,DIAS N P,GALDEANO D M,et al.Management of pest insects and plant diseases by non-transformative RNAi[J].Frontiers in plant science,2019,10:1319.
[106] WEN H G,ZHAO J H,ZHANG B S,et al.Microbe-induced gene silencing boosts crop protection against soil-borne fungal pathogens[J].Nature plants,2023,9(9):1409-1418.
[107] NEUPANE A,FENG C C,MOCHAMA P K,et al.Roles of argonautes and dicers on Sclerotinia sclerotiorum antiviral RNA silencing[J].Frontiers in plant science,2019,10:976.
[108] GAMMON D B,ISHIDATE T,LI L C,et al.The antiviral RNA interference response provides resistance to lethal arbovirus infection and vertical transmission in Caenorhabditis elegans[J].Current biology,2017, 27(6):795-806.
[109] ROMEIS J,WIDMER F.Assessing the risks of topically applied dsRNA-based products to non-target arthropods[J].Frontiers in plant science,2020,11:679.
[110] DARSAN SINGH J K,MAT JALALUDDIN N S,SANAN-MISHRA N,et al. Genetic modification in Malaysia and India: Current regulatory framework and the special case of non-transformative RNAi in agriculture[J]. Plant cell reports,2019,38(12):1449-1463.
[111] CHRISTIAENS O,DZHAMBAZOVA T,KOSTOV K,et al.Literature review of baseline information on RNAi to support the environmental risk assessment of RNAi-based GM plants[J].EFSA supporting publications,2018,15(5):1424E.
[112] RODRIGUES T B,PETRICK J S.Safety considerations for humans and other vertebrates regarding agricultural uses of externally applied RNA molecules[J].Frontiers in plant science,2020,11:407.
[113] TANING C N,ARPAIA S,CHRISTIAENS O,et al.RNA-based biocontrol compounds: Current status and perspectives to reach the market[J].Pest management science,2020,76(3):841-845.