Preparation of Cerasus humilis Kernel Peptide and Assessment of the Antioxidant Activity in vivo

doi:10.14088/j.cnki.issn0439-8114.2018.21.025

Abstract

Abstract: Kernel of Cerasus humilis protein were hydrolyzed by three kinds of enzymes including alkaline protease, trypsin and neutral proteinase. Kernel of Cerasus humilis peptide of different concentrations were gaved in the mice. Determined the antioxidant of the normal mice and model mice by analyzing the body weight,the SOD activity,the GSH-PX activity and the MDA concentration in serum,liver and kidney of mice. Results suggest that the kernel of Cerasus Humilis peptides had a relatively high level of DPPH radical scavenging activity with the IC₅₀ value was 1.06 mg/mL. The high dose peptide could significantly increase the SOD and GSH-PX activity in serum,liver and kidney and could significantly reduce the concentration of MDA in serum,liver （P<0.05）,but the effect on MDA in kidney was not significantly.

Key words: kernel of Cerasus Humilis, hydrolysis, DPPH free radical, antioxidant activity

CLC Number:

R282

ZHANG Ling, HAO Li-ping. Preparation of Cerasus humilis Kernel Peptide and Assessment of the Antioxidant Activity in vivo[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(21): 101-104.

References 11

[1]	杜俊杰,杨怀毅,池建伟.山西省欧李资源的分布及类群[J].作物品种资源,1993(2):6-7.
[2]	阴健. 中药现代研究与临床应用[M].北京:中医古籍出版社,1993.
[3]	刘淑琴,常虹,周家华,等.我国欧李的开发应用研究现状[J].食品研究与开发,2009,30(12):167-170.
[4]	薛勇,陈玮,程霜,等.双相溶剂浸出法提取欧李仁油的研究[J].郑州工程学院学报,2003,24(1):20-23.
[5]	陈玮,王宏雁,薛勇,等.欧李仁蛋白的提取与性能研究[J].食品科学,2005,26(3):138-141.
[6]	张美莉,邓秋才,杨海霞,等.内蒙古欧李果肉和果仁中营养成分分析[J].氨基酸和生物资源,2007,29(4):18-20.
[7]	王晓坤. 超声波辅助酶解制备花生抗氧化肽的研究[D].郑州:河南工业大学,2012.
[8]	REN H F,LIU H,HIDEAKI E,et al.Anti-mutagenic and anti-oxidative activities found in Chinese traditional soybean fermented products furu[J].Food Chemistry,2006,95(1):71-76.
[9]	KATSUHIRO KUDO,SHUICHI ONODERA,YASUYUKI TAKEDA,et al.Antioxidative activity of some peptides isolated from hydrolyzed potato protein extract[J].Journal of Function Foods,2009,170-176.
[10]	荣建华. 大豆肽和其生物活性的研究[D].武汉:华中农业大学, 2001.
[11]	IMELDA W Y,CHEUNG,LENNIE K Y,et al. The role of molecular size in antioxidative activity of peptides fraction from pacific hake hydrolysates[J].Food Chemistry,2012,134:1297-1306.

[1]	DAI Lu, ZHANG Zi-yi, XUE Wen, SHEN Jing, TANG Li-li, LONG Ming-hua. Preparation and properties of polylactic acid/oregano essential oil antioxidant active packaging film [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 110-112.
[2]	YANG Jin-hong, CHEN Zheng-yu, MENG Gang, KONG Wei-qing. Determination of functional components and antioxidant activities of mulberry juice and mulberry wine [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 117-119.
[3]	YAN Dan, CAI Yan-qu, LI Tie-cheng, CHEN Bi-tian, FENG Hua-zi, ZHANG Zhi-peng. Optimization extraction through Box-Behnken response surface methodology and antioxidant activity in vitro of polysaccharides from aerospace hybrid Broussonetia papyrifera leaves [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 100-105.
[4]	ZHAO Nan, JING Yun-rong, HE Ting-ting, CHAI Jun-hong, WAN Chun-lei, ZHANG Lei. Study on extracting and antioxidant activity of total flavonoids from Evodia rutaecarpa [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(3): 118-121.
[5]	LI Xiao-na, JI Yong-hui, WANG Jing-long, ZHANG Li-hua, ZANG Yuan-long, YANG Bin, LIU Chun-he, LIU Chun-jiang. Study on antioxidant activity and HPLC fingerprints from different polarity parts of Oxalis corniculata L. [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(24): 156-163.
[6]	LI Xiao-ning, XU Xing-yang, FAN Mao-pan, YANG Qing-song, ZHAO Yan. Changes of polyphenol content and related enzyme activities in different leaf positions of tobacco [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(9): 80-85.
[7]	HUANG Jing-ling, JIANG Han-mei, TU Han, MENG Jia-min, XIAO Yu-shuo. Study on antioxidant activity of Eugenia cargophyllata Thunb. [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(9): 117-119.
[8]	LI Xiao-fen, XIONG Hua-bin, ZHANG Hai-fen, ZI Yuan-li, ZHANG Yan, GAO Yun-tao, ZHANG Yan-li. Spectrometric investigation of the antioxidant activities of Terminalia polyphenol against DPPH free radicals [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(8): 121-125.
[9]	ZHAO Yun-shu, CHENG Jing, LU Zi-bo, LI Xuan, CHEN Wen-wen, HUANG Liu-rong. Extraction of soluble dietary fiber from garlic straw via ultrasonic-assisted enzymatic hydrolysis and evaluation of antioxidant activity [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(6): 117-120.
[10]	CHAI Jun-hong, HE Ting-ting, ZHAO Nan, JIN Zhi-min. Study on antioxidant and antitumor activity of polysaccharide and anthocyanin from Parthenocissus quinquefolia fruit [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(15): 97-101.
[11]	HUANG Su-hua, CHEN Tong, QIU Feng-yan, HONG Yan-ping. Research on antioxidant and antitussive and expectorant activity of different components from loquat leaves [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 138-140.
[12]	FENG Tao, ZHOU Guo-hua, Chi Feng-long, WANG Bang-jie. Improvement of subcritical hydrolysis of organic waste on coastal saline alkali soil in Tianjin [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(10): 74-77.