Abstract: In vitro the antioxidant activity of polysaccharide and anthocyanin on Parthenocissus quinquefolia fruit was discussed. Study on the scavenging ability of polysaccharide and anthocyanin with superoxide free radical, hydroxyl radical and DPPH free radical. Using MTT method to study the anthocyanins and polysaccharides in inhibition of 2 kinds of hepatoma cells（HepG2, SMMC-7221）. The results showed that the polysaccharide and anthocyanin concentration has a dose-dependent relationship with the HCC inhibitory rate, when the anthocyanin concentration reached 0.312 5 mg/mL, the inhibition rate of two HCC was 37.89%±1.74%, 53.57%±1.72%; when the polysaccharide concentration reached 0.312 5 mg/mL, the inhibition rate of two HCC cells was 79.59%±0.52%, 58.85%±1.12%. The polysaccharide or anthocyanin was 0.80 mg/mL, the scavenging rate of superoxide radicals was 74.21%±2.48%、90.06%±0.86%, the hydroxyl radical was 75.87%±1.61%、68.60%±1.52%, the scavenging rate of DPPH was 91.41%±0.74%、90.73%±0.44%. Furthermore, the scavenging ability of hydroxyl radical has a correlation with antitumor. The superoxide radicals also showed certain correlation, and the correlation of DPPH was poor. Parthenocissus quinquefolia fruit polysaccharide and anthocyanin has certain inhibitory effect on HCC and radical, there is a clear correlation with antioxidant and antitumor;it has the development potential.

Key words: Parthenocissus quinquefolia fruit, anthocyanin, polysaccharide, antioxidant activity, MTT