Cloning and Expression Analysis of 3-dehydroquinate Synthase Gene in Pomegranate

doi:10.14088/j.cnki.issn0439-8114.2018.18.026

Abstract

Abstract: The 3-dehydroquinate synthase（DHQS） cDNA sequence was cloned from Taishanhong pomegranate（Punica granatum L.） pericarp with RT-PCR technique. The expression of DHQS gene was analyzed in different pomegranate cultivars fruits during development. The result showed that the cDNA sequence length of DHQS was 273 bp,which encoded 91 amino acids. The protein of DHQS gene contained DHQ_Fe_ADH superfamily conservative structure domain. The similarity of DHQS nucleotide sequence in pomegranate and Fagus sylvatica,Prunus mume,Medicago truncatula were 91%,87% and 88%,respectively. The similarity of DHQS deduced amino acid sequences in pomegranate and Fagus sylvatica,Malus domestica,Pyrus x bretschneideri,Arabidopsis thaliana were 95%,92%,92% and 88%,respectively. The relative expression level of DHQS gene decreased gradually with the fruit development in two pomegranate cultivars,which peak value appeared at July 15^th. The relative expression level of DHQS gene in Taishanhong was higher than that in Taishansanbaitian in the whole development period.

Key words: Punica granatum L., 3-dehydroquinate synthase, cloning, expression analysis

CLC Number:

S665.4

YIN Yan-lei, TAO Ji-han, YANG Xue-mei, TANG Hai-xia, FENG Li-juan. Cloning and Expression Analysis of 3-dehydroquinate Synthase Gene in Pomegranate[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(18): 103-105.

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