Influence on Rice Anther Culture Process on Culture Result

doi:10.14088/j.cnki.issn0439-8114.2018.24.044

Abstract

Abstract: There are many factors which influence the result of rice anther culture. In this study, Yuzhenxiang/Ezhongwuhao/Yujing91 was used to reveal the influence of rice anther culture process on culture result. Young panicles pretreated in low temperature for 8 days had a good induction rate if sucrose was added into the induction medium while pretreatment with ten days had a better result if maltose was added into the induction medium, on the whole, maltose was better than sucrose in the induction period. The time of drawing young panicles effected the induction rate and too early and too late were disadvantageous to callus induction. If calluses were induced with the medium with maltose, there would have better differentiation rate than medium with sucrose. Calluses which were induced early had much higher differentiation rate than those were lately induced.

Key words: rice （Oryza sativa L.）, anther culture, induction rate, differentiation rate

CLC Number:

S511

LI San-he, ZHA Wen-jun, ZHOU Lei, LIU Kai, YANG Guo-cai, YOU Ai-qing. Influence on Rice Anther Culture Process on Culture Result[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(24): 164-166.

References

[1] 朱旭东,曾大力,斯华敏,等.标记两用核不育水稻M2S的花药培养及遗传分析[J].中国水稻科学,2002,16(2):24-128.
[2] 曾千春,吴茜,冯德江,等.转修饰cry1Ac基因籼稻明恢81经花药培养获得抗虫DH系[J].生物工程学报,2002,18(4):442-445.
[3] 黄翠红,杨瑰丽,黄明,等.两系不育系Y58S的花药培养与花培后代的鉴定筛选[J].广东农业科学,2016,43(5):31-36.
[4] 葛胜娟. 水稻花药培养及其在遗传育种上的应用[J].种子,2013, 32(8):45-50.
[5] 葛胜娟. 水稻花药培养及其在遗传育种上的应用[J].种子,2013, 32(9):47-50.
[6] 李三和,陈志军,刘凯,等.籼稻不育系花药培养诱导条件探讨[J].湖北农业科学,2015,54(19):4868-4870.
[7] 孙宗修,斯华敏,程式华.麦芽糖提高水稻花培效率的研究[J].中国水稻科学,1993,7(4):227-231.
[8] RAINA S K,IRFAN S T.High frequency embryogenesis and plantlet regeneration from isolated microspores of rice[J].Plant Cell Reports,1998,17:957-962.
[9] GABRIELA T T,URIEL M A,GUADALUPE S M.The effects of cold-pretreatment,auxins and carbon source on anther culture of rice[J].Plant Cell Tissue and Organ Culture,2002,71:41-46.
[10] MARIA A G.Anther culture for haploid and doubled haploid production[J].Plant Cell Tissue and Organ Culture,2011,104:283-300.
[11] 冯双华,赵森,郭家源,等.不同培养基和激素对超级杂交稻花药培养力的影响[J].西南农业大学学报(自然科学版),2006,28(4):523-525,565.
[12] 王伍梅,台德卫,张效忠,等.水稻高效花药培养技术体系的构建[J].中国农学通报,2009,25(16):65-68.
[13] 向发云,宋志红,吴金平,等.籼稻材料特性和激素配比对花药培养效率的影响[J].湖北农业科学,2008,47(12):1380-1384.
[14] 刘凯,唐红生,严国红,等.籼稻复交F₁花药培养力的提高技术[J].江苏农业科学,2017,45(4):47-49.
[15] 刘凯,唐红生,严国红,等.提高籼稻恢复系花药培养绿苗分化率的研究[J].河南农业科学,2016,45(10):32-34.
[16] 孙杰,马凌霄.水稻花药培养适宜条件的相关研究[J].北方水稻,2016,47(1):35-37.
[17] 沈锦骅,李梅芳,陈银全,等.花药培养在水稻品种改良上的应用[J].中国农业科学,1982(2):15-19.
[18] 赵成章. 水稻试管苗正常化调控技术的研究[A].胡古,王恒立.植物细胞工程与育种[M].北京:北京工业大学出版杜,1990.
[19] 冯双华,侯海军,廖江林,等.不同因素对水稻温敏核不育系香125S花药培养的影响[J].农业现代化研究,2007,28(3):358-360.
[20] 丁世萍,严菊强,季道藩.糖类在植物组织培养中的效应[J].植物学通报,1998(6):42-46.
[21] 罗琼,曾千春,周开达,等.水稻花药培养及其在育种中的应用[J].杂交水稻,2000,15(3):l-2.