RAPD Analysis on Genetic Diversity of Cardamine violifolia O. E. Schulz from Different Dregion in Hubei and Hunan Provinces

doi:10.14088/j.cnki.issn0439-8114.2018.24.043

HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (24): 160-163.doi: 10.14088/j.cnki.issn0439-8114.2018.24.043

• Biological Engineering • Previous Articles Next Articles

RAPD Analysis on Genetic Diversity of Cardamine violifolia O. E. Schulz from Different Dregion in Hubei and Hunan Provinces

XIANG Ji-qian^1,2,3, WANG Xiao-hui^1,2, YIN Hong-qing^1,2,3, WAN Hai-ying^1,3, LI Ya-jie^1,3, YANG Yong-kang^1,3, CONG xin⁴

1.Enshi Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000,Hubei,China;
2.Hubei Selenium Industrial Technology Research Institute, Enshi 445000,Hubei,China;
3.West Comprehensive Experiment Station of Hubei Provincial S&T Innovation Center,Enshi 445000,Hubei,China;
4.Deyuan Health Industry Research Institute,Enshi 445000,Hubei,China

Received:2018-09-14 Online:2018-12-25 Published:2020-04-01

Abstract

Abstract: Random amplified polymorphic DNA（RAPD） analysis was applied to determine the genetic diversity among 5 Cardamine violifolia O. E. Schulz samples from different region of Hubei and Hunan provinces. 10 among 50 primers were selected to amplify about 78 segments DNA, 49 polymorphic segments were found, the percentage of polymorphic probability was 62.8%. Cluster analysis using NTSYS software, a similar coefficient of 0.68 as the standard, all varieties can be divided into three categories. GroupⅠincluding C.violifolia Hubei “1” and Yutangba Shuanghe of Enshi City; GroupⅡ including Sifangdong Changyang Yichang City and Hupingshan Changde City; Liziping Wufeng Yichang City was divided into a class of independent.

Key words: Cardamine. violifolia O. E. Schulz, RAPD, genetic diversity

CLC Number:

XIANG Ji-qian, WANG Xiao-hui, YIN Hong-qing, WAN Hai-ying, LI Ya-jie, YANG Yong-kang, CONG xin. RAPD Analysis on Genetic Diversity of Cardamine violifolia O. E. Schulz from Different Dregion in Hubei and Hunan Provinces[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(24): 160-163.

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RAPD Analysis on Genetic Diversity of Cardamine violifolia O. E. Schulz from Different Dregion in Hubei and Hunan Provinces

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