Abstract: Through the deep hole plate germination of Brassica napus hybrid, the steps of DNA extraction by alkali pyrolysis were simplified, and the application of high throughput grinder Fast & Fluid Management SK300 sample homogenizer was combined to provide a whole set of intensive scheme for rapidly obtaining cotyledon DNA samples of rapeseed hybrid with high throughput and low cost. Using this method, 10 thousand DNA could be obtained in 10 hours, which improve the efficiency of DNA extracting, significantly and facilitate purity identification of hybrid rape seed with molecular marker, effectively.

Key words: rape（Brassica napus）, hybrid, DNA extraction, molecular marker, purity identification