Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia

doi:10.14088/j.cnki.issn0439-8114.2020.22.033

Abstract

Abstract: Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia,Fritillaria hupehensis Hsiao et k.c.Hsia were collected from Enshi area,Hubei province,China,whoes total extract was obtained by ultrasonic extraction with ethanol solution (60%).And the total extract to obtain petroleum ether segment were extracted.The antibacterial activity and mechanism of petroleum ether segment of Fritillaria hupehensis were studied by means of filter paper method,dilution pour plate method,ultra micro ultraviolet spectrophotometer and conductivity meter.The composition of petroleum ether segment from Fritillaria hupehensis was studied by means of GC-MS,1D and2D NMR.Results showed that Hubei Fritillaria petroleum ether solution was moderately inhibitory sensitive on Bacillus thuringiensis and Pseudomonas aeruginosa,and mildly inhibitory sensitive on Klebsiella pneumonia ATCC13883,Staphylococcus aureus ATCC29213,and Bacillus subtilis.Among them,the MIC/MBC of Bacillus thuringiensis,Bacillus subtilis,and Pseudomonas aeruginosa were less than4.0 mg/mL.Through the exploration of antibacterial mechanism,the petroleum ether section of Fritillaria fragrans Hubei probably slowed the growth of Bacillus thuringiensis,Bacillus subtilis,Pseudomonas aeruginosa by destroying the cell membrane,and finally achieved antibacterial effect.This study also explored the composition of the petroleum ether Extracts from Fritillaria hupehensis Hsiao et k.c.Hsia.Total ion chromatograms of the petroleum ether Extracts from Fritillaria hupehensis Hsiao et k.c.Hsia were obtained and compared with the NIST08.LIB standard library based on retention time and mass spectrometry data.Finally27 compounds of the total ion chromatogram were obtained.

Key words: Fritillaria hupehensis Hsiao et k.c.Hsia, antimicrobial activity, petroleum ether extract, compound

CLC Number:

FANG Shi-qi, LI Yu, HUANG Dong-hai, AI Lun-qiang, LIU Cui-jun, HE Mei-jun. Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 160-165.

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