Screening and identification of endophytic antimicrobial strains from Dendrobium changjiangense

doi:10.14088/j.cnki.issn0439-8114.2022.15.021

Abstract

Abstract: Ten endophytic bacteria were isolated from Dendrobium changjiangense by tissue block method and streak method, and three indicator bacteria including Escherichia coli, Staphylococcus aureus and Candida albicans were used to study the antimicrobial activity of endophytic bacteria isolated from Dendrobium changjiangense by agar plate method. The results showed that among the 10 endophytic bacteria isolated from Dendrobium changjiangensis, the root was the most, followed by the stem, and the leaf was the least. Among the 10 endophytic bacteria isolated from Dendrobium changjiangensis, two strains had inhibitory effects on at least one test bacterium, accounting for 20% of the total isolated strains. Among them, C-06 strain had inhibitory effects on the three indicator bacteria, and one strain C-05 had inhibitory effects on the two indicator bacteria, respectively. Through the analysis of bacterial 16S rRNA gene sequence, the active strains were distributed in Bacillus and Bacillus-like. Dendrobium changjiangense is rich in endophytic bacteria resources. Some endophytic bacteria have the characteristics of metabolic inhibition, which can be used as a screening source of new drugs and provide a reference for the treatment of clinical diseases.

Key words: Dendrobium chanjiangensis, endophytic bacteria, antimicrobial activity, genus identification

CLC Number:

Q946

GAO Fang-mei, CHEN Xia-shi, CHEN Mao-nan, GAO Fang-jian, YANG Wen. Screening and identification of endophytic antimicrobial strains from Dendrobium changjiangense[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 124-128.

References

[1] 王楠,苏誉,刘文杰,等.植物内生菌中抗耐药微生物活性成分的研究进展[J].生物技术通报,2021,37(8):263-274.
[2] 方珍娟,张晓霞,马立安.植物内生菌研究进展[J]. 长江大学学报(自然科学版),2018,15(10):41-45.
[3] MOURAD M H E S. New bioactive compounds from verticillium alboatrum and verticillium leptobactrum[J]. Australian journal of basic & applied sciences,2010,4(8):2166-2175.
[4] JOUDA J B, MAWABO I K, NOTEDJI A, et al.Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacteriumsmegmatis[J]. International journal of mycobacteriology,2016,5(2):192-196.
[5] 梁卉,李联萍,欧阳敏,等. 药用植物内生真菌及其生物活性物质研究概况[J]. 广东化工,2011,38(5):50-51,29.
[6] 曹森.王婷,高天.叶底珠内生真菌提取物的抗炎镇痛和抗氧化活性研究[J]. 药物评价研究,2021,44(6):1232-1239.
[7] MAYER E,QUADROS P D,FULTHORPE R.Plantibacter flavus, Curtobacterium herbarum,Paenibacillus taichungensis,and Rhizobium selenitireducens endophytes provide host-specific growth promotion of Arabidopsis thaliana, Basil, Lettuce, and Bok choy plants[J]. Applied and environmental microbiology,2019,85(19):e00383-19.
[8] 杨志军,邓毅,曼琼,等.内生菌在天然药物研究中的研究进展[J]. 中国临床药理学杂志,2018,34(5):593-596.
[9] GOS F M W R, SAVI D C, SHAABAN K A, et al. Antibacterial activity of endophytic actinomycetes isolated from the medicinal plant Vochysia divergens(Pantanal, Brazil)[J]. Frontiers in microbiology,2017,8(8):1-17.
[10] ALSHAIBANI M, ZIN N M, JALIL J, et al.Isolation, purification, and characterization of five active diketopiperazine derivatives from endophytic Streptomyces SUK 25 with antimicrobial and cytotoxic activities[J]. Journal of microbiology and biotechnology, 2017,27(7):1249-1256.
[11] VU H N T, NGUYEN D T, NGUYEN H Q, et al. Antimicrobial and cytotoxic properties of bioactive metabolites produced by Streptomyces cavourensis YBQ59 isolated from Cinnamomum cassia prels in Yen Bai province of Vietnam[J]. Current microbiology,2018,75(10):1247-1255.
[12] TANG H X, ZHAO T W, SHENG Y J.et al.Dendrobium officinale Kimura et Mi-go: A review on its ethnopharmacology,phytochemistry,pharmacology,and industrialization[J]. Evidence-based complementary and alternative medicine,2017(5):1-19.
[13] 陶泽鑫,陆宁姝,吴晓倩,等.石斛的化学成分及药理作用研究进展[J].药学研究,2021,40(1):44-51,70.
[14] 吴庆珊,雷珣,雷友梅,等.金钗石斛内生细菌的组成及多样性分析[J]. 植物资源与环境学报,2018,27(1):79-90.
[15] WANG S S,LIU J M,SUN J,et al.Diversity of culture-independent bacteria and antimicrobial activity of culturable endophytic bacteria isolated from different Dendrobium stems[J].Scientific reports,2019,9(1):10389.
[16] 吴德邻. 海南及广东沿海岛屿植物名录[M].北京:科学出版社, 1994.
[17] 许洋瑜,罗文,韩文涛,等.海南岛霸王岭极小种群野生植物调查初报[J].热带林业,2014,42(2):32-36.
[18] 杨柳,滕李濛,黄内力,等. 海南石斛与昌江石斛体外抗菌活性试验研究[J]. 热带农业科学,2018,38(8):56-63.
[19] 赵静. 杜仲内生细菌的分离鉴定与活性菌株筛选[D]. 河南开封:河南大学,2017.
[20] 柴晓蕾, 宋希强, 朱婕. 华石斛内生真菌组织分布特点及其抑菌活性[J]. 热带作物学报,2018,39(1):137-144.
[21] 王珊珊,刘佳萌,孙晶,等.药用石斛内生菌的研究进展[J].中国食物与营养,2020,26(7):35-40,25.
[22] 高阳,朱双丽, 吴庆珊,等.金钗石斛IAA内生细菌筛选及对干旱胁迫下玉米种子萌发的影响[J].种子,2017,36(4):36-41.
[23] RAMALINGAM R,HASHEM A, ALLAH E F A. Bacillus: A biological tool for crop improvement through bio-molecular changes in adverse environments[J]. Front Physiol,2017,8: 667.
[24] 靳锦,赵庆,张晓梅,等. 植物内生菌活性代谢产物最新研究进展[J]. 微生物学杂志,2018,38(3):103-113.
[25] PEI C,MI C Y,SUN L H,et al.Diversity of en-dophytic bacteria of Dendrobium officinale based on culture-dependent and culture-independent methods[J]. Biotechnology and biotechnological equipment,2016,31(1):112-119.