Identification of An Actinomyce Isolate JK-1 and Its Antifungal Activity Against the Rice Blast Fungus

doi:10.14088/j.cnki.issn0439-8114.2018.24.027

Abstract

Abstract: Morphological,physiological and biochemical characteristics and 16S rRNA gene sequence of actinomyce isolate JK-1 were analysed. It showed that isolate JK-1 belonged to Streptomyces albidoflavus var. . To evaluate the inhibiting activities of isolate JK-1 against four plant fungal pathogens,the rice blast fungus（Magnaporthe oryzae）,the cotton verticillium wilt pathogen （Verticillium alboatrum）,the fusarium head blight pathogen （Fusarium graminearum） and rice sheath blight pathogen （Rhizoctonia solani Kühn）,antifungal activity assays were performed using mycelium growth rate method. The crude fermentation liquid showed strong inhibiting activity against the four pathogens,especially M. oryzae with inhibitory rate of 91.14%±0.04%. The crude fermentation liquid was concentrated by freeze drying,and then extracted with methanol. The diluted methanol extraction of fermentation liquid with 2 000 times exhibited a significant inhibitory effect on mycelium growth of M. oryzae with inhibiting rate of 53.42%±1.29%.

Key words: Streptomyces, classification and identification, the rice blast fungus （Magnaporthe oryzae）, antimicrobial activity

CLC Number:

S435.111.4⁺1

ZENG Fan-song, LI Su-qin, WANG Yun-feng, LIU Qi-mei, XIANG Li-bo, YANG Li-jun, GONG Shuang-jun, SHI Wen-qi. Identification of An Actinomyce Isolate JK-1 and Its Antifungal Activity Against the Rice Blast Fungus[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(24): 96-99.

References

[1] SKAMNIOTI P,GURR S J.Against the grain:Safeguarding rice from rice blast disease[J].Trends Biotechnol,2009,27(3):141-150.
[2] POOJA K,KATOCH A.Past,present and future of rice blast management[J].Plant Sci Today,2014,1(3):165-173.
[3] 吴志华,李苹,柏连阳,等.湖南省水稻稻瘟病菌对稻瘟灵抗药性研究[J].安徽农业科学,2011,39(28):17279-17280.
[4] 姚树萍,贾丽,何玲,等.我国水稿稻瘟病菌对三环唑抗性研究现状[J].农药科学与管理,2017,38(6):13-17.
[5] LAW J W,SER H L,KHAN T M,et al.The potential of Streptomycesas biocontrol agents against the rice blast fungus, Magnaporthe oryzae(Pyricularia oryzae)[J].Front Microbiol,2017, 8(3):1-10.
[6] 张炳火,李汉全,徐阳,等.链霉菌JXJ402抗稻瘟病菌的活性成分[J].江苏农业学报,2009,25(2):282-285.
[7] 廖红东,袁珊珊,杨远柱,等.一株拮抗稻瘟病内生链霉菌OsiSh-10的筛选与鉴定[J].湖南大学学报(自然科学版),2015,42(12):80-87.
[8] 邵正英,陈业欣,熊超,等.链霉菌JD211对接种稻瘟病后水稻过敏性反应的生理机制分析[J].西北植物学报,2017,37(7):1340-1347.
[9] 王真真,徐婷,袁珊珊,等.水稻内生放线菌OsiRt-1的分离鉴定及对稻瘟病的防治作用[J].微生物学通报,2016,43(5):1009-1018.
[10] XU B,CHEN W,WU Z M,et al.A novel and effective Streptomyces sp. N2 against various phytopathogenic fungi[J].Appl Biochem Biotech,2015,177(6):1338-1347.
[11] PRABAVATHY V R,MATHIVANAN N,MURUGESAN K.Control of blast and sheath blight diseases of rice using antifungal metabolites produced by Streptomyces sp. PM5[J].Biol Control,2006,39(3):313-319.
[12] 张学江,曾凡松,杨立军,等.抗稻瘟病链霉菌JK-1基因组BAC文库构建[J].安徽农学通报,2014,20(24):17-18,129.
[13] 中国科学院微生物研究所放线菌分类组.链霉菌鉴定手册[M].北京:科学出版社,1975.
[14] 阎逊初. 放线菌的分类和鉴定[M].北京:科学出版社,1992.
[15] WILLIAMS S T,DAVIES F L.Use of a scanning electron microscope for the examination of actinomycetes[J].J Gen Microbiol,1967,48(2):171-177.
[16] 张志斌,敖武,熊瑶瑶,等.内生拮抗放线菌FRo2的鉴定及抑菌活性物质的分离[J].微生物学通报,2014,41(8):1574-1581.
[17] NIGTHOUJAM D S,SANASAM S,TAMREIHAO K,et al.Antagonistic activities of local actinomycete isolates against rice fungal pathogens[J].Afr J Microbiol Res,2009,3(11):737-742.
[18] BOUKAEW S,PRASERTSAN P.Suppression of rice sheath blight disease using a heat stable culture filtrate from Streptomyces philanthi RM-1-138[J].Crop Prot,2014,61(3):1-10.
[19] KHALIL M S,MOUBASHER H,HASAN F F.Biological control of rice blast disease by Streptomyces flavotricini[J].Res J Pharm Biol Chem Sci,2014,5(5):1453-1461.
[20] QIN S,LI J,CHEN H H,et al.Isolation,diversity,and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna,China[J].Appl Environ Microbiol,2009,75(19):6176-6186.
[21] 周美英,郑志成.海洋放线菌S-216菌株鉴定及其抗真菌抗生素合成条件研究[J].厦门大学学报(自然科学版),1998,37(1):109-114.
[22] 张永娜,刘佳琳,徐德阳,等.2株微白黄链霉菌发酵液的稳定性及抑菌效果分析[J].中国农学通报,2017,33(12):108-114.
[23] 于素亚,潘华奇,胡江春,等.深海放线菌08A4的鉴定及其抗真菌活性产物研究[J].微生物学杂志,2012,32(5):1-5.