Analysis of tillering characteristics, related-nitrogen assimilation enzyme activity and gene expression in rice

doi:10.14088/j.cnki.issn0439-8114.2020.23.039

Abstract

Abstract: Tillering of rice is one of the important agronomic traits that affect the number of panicles and thus the yield of rice.It was found that low-node (early-onset) tillers had higher yield per panicle than high-node (late-onset) tillers.12 rice varieties with different tillering characteristics were used as meterials.Based on the observation of tiller development dynamics under hydroponics conditions,ammonium nitrogen assimilation enzyme activity and rice tillering-related gene expression were analyzed.Correlation analysis found that the number of tillers was significantly positively correlated with the tillering rate in the second and third leaf positions,but no significant correlation between tiller number and tiller rate at the frist leaf position in rice.The tillering rate at the first leaf position was mainly affected by the ammonium assimilation enzyme activity in rice seedlings,while the expression of genes related to the auxin and monopetalactone signaling pathway did not significantly affect it.The second and third leaf tillering rate were relatively weakly affected by the seedling ammonium nitrogen assimilation enzyme activity and gene expression,but was mainly negatively regulated by the expression of genes related to the auxin and strigolactone signaling pathways.Besides,the soluble sugar content of rice seedlings,genes such as MOC1 that control tiller bud formation and genes that control CK synthesis were not significantly correlated with the tillering rate in the first,second and third leaf positions,while genes such as TB1 that regulate tiller bud elongation had a more significant effect on it.

Key words: rice (Oryza sativa L.), tiller, ammonium assimilation, hormone, gene expression

CLC Number:

S511

AN Qi, JI Wen-jun, HE Yong-gang, ZHANG Zhi-hong. Analysis of tillering characteristics, related-nitrogen assimilation enzyme activity and gene expression in rice[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(23): 174-179.

References

[1] SAKAMOTO T,MATSUOKA M.Identifying and exploiting grain yield genes in rice[J].Current opinion in plant biology,2008,11(2):209-214.
[2] LI X Y,QIAN Q,FU Z M,et al.Control of tillering in rice[J].Nature,2003,422(6932):618-621.
[3] 毛礼钟.水稻蘖间养分交流的初步观察[J].浙江师范大学学报(自然科学版),1989,12(2):116-125.
[4] 凌启鸿.作物群体质量[M].上海:上海科学技术出版社,2000.
[5] 刘瑞华,崔贞玉,冯权,等.水稻分蘖期的温度条件与适宜栽培密度的研究[J].吉林农业科学,1993(2):40-43.
[6] 丁艳锋,黄丕生,凌启鸿.水稻分蘖发生及与特定部位叶片叶鞘含氮率的关系[J].南京农业大学学报,1995,18(4):14-18.
[7] 蒋彭炎,马跃芳,洪晓富,等.水稻分蘖芽的环境敏感期研究[J].作物学报,1994,20(3):290-296.
[8] LAM H M,COSCHIGANO K T,OLIVEIRA I C,et al.The molecular-genetics of nitrogen assimilation into amino acids in higher plants[J].Annual review of plant biology,1996,47:569-593.
[9] TABUCHI M,ABIKO T,YAMAYA T.Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.)[J].Journal of experimental botany,2007,58(9):2319-2327.
[10] LIU Y,GU D D,DING Y F,et al.The relationship between nitrogen,auxin and cytokinin in the growth regulation of rice (Oryza sativa L.) tiller buds[J].Australian journal of crop science,2011,5(8):1019-1026.
[11] MAE T.Physiological nitrogen efficiency in rice:Nitrogen utilization,photosynthesis,and yield potential[J].Plant and soil,1997,196:201-210.
[12] TANAKA W,OHMORI Y,USHIJIMA T,et al.Axillary meristem formation in rice requires the WUSCHEL ortholog TILLERS ABSENT1[J].The plant cell,2015,27(4):1173-1184.
[13] LIN Q B,WANG D,DONG H,et al.Rice APC/C(TE) controls tillering by mediating the degradation of MONOCULM 1[J].Nature communications,2012,3:752-766.
[14] XU C,WANG Y,YU Y,et al.Degradation of MONOCULM 1 by APC/CTAD1 regulates rice tillering[J].Nature communications,2012,3:750-768.
[15] SU’UDI M,CHA J Y,JUNG M H,et al.Potential role of the rice OsCCS52A gene in endore duplication[J].Planta,2012,235(2):387-397.
[16] ARITE T,IWATA H,OHSHIMA K,et al.DWARF10,an RMS1/MAX4/DAD1 ortholog,controls lateral bud outgrowth in rice[J].The plant journal,2007,51(6):1019-1029.
[17] KULKARNI K P,VISHWAKARMA C,SAHOO S P,et al.A substitution mutation in OsCCD7 cosegregates with dwarf and increased tillering phenotype in rice[J].Journal of genetics,2014,93(2):389-401.
[18] ISHIKAWA S,MASAHIKO M,TOMOTSUGU A,et al.Suppression of tillerbud activity in tillering dwarf mutant of rice[J].Plant cell physiol,2005,46(1):79-86.
[19] ARITE T,UMEHARA M,ISHIKAWA S,et al.d14,a strigolactone-insensitive mutant of rice,shows an accelerated outgrowth of tillers[J].Plant cell physiol,2009,50(8):1416-1424.
[20] GAO Z,QIAN Q,LIU X H,et al.Dwarf 88,a novel putative esterase gene affecting architecture of rice plant[J].Plant molecularbiology,2009,71(3):265-276.
[21] LIU W Z,WU C,FU Y P,et al.Identification and characterization of HTD2:A novel gene negatively regulating tiller bud outgrowth in rice[J].Planta,2009,230(4):649-658.
[22] LU Z F,YU H,XIONG G S,et al.Genome-Wide binding analysis of the transcription activator IDEAL PLANT ARCHITECTURE1 reveals a complex network regulating rice plant architecture[J].Plant cell,2013,25(10):3743-3759.
[23] LEYSER O.Regulation of shoot branching by auxin[J].Trends in plant science,2003,8(11):541-545.
[24] CHEN C Y,ZHOU J H,ZHANG S Y,et al.Strigolactones are a new defined class of plant hormones which inhibit shoot branching and mediate the interaction of plant-AM fungi and plant-parasitic weeds[J].Science in China series C:Life sciences,2009,52(8):693-700.
[25] LEYSER O.The fall and rise of apical dominance[J].Current opinion in genetics and development,2005,15(4):468-471.
[26] DOMAGALSKA M A,LEYSER O.Signal integration in the control of shoot branching[J].Nature reviews molecular cell biology,2011,12(4):211-221.
[27] 毛达如.植物营养研究方法[M].北京:中国农业大学出版社,2005.
[28] 卢永恩.水稻谷氨酸合酶基因和胞质异柠檬酸脱氢酶基因的功能研究以及氨基酸转运蛋白基因家族分析[D].武汉:华中农业大学,2014.
[29] RHODES D,RENDO G A,STEWART G R.The control of glutamine synthetase level in Lemna minor L.[J].Planta,1975,125(3):201-211.
[30] TURANO F J,DASHNER R,UPADHYAYA A,et al.Purification of mitochondrial glutamate dehydrogenase from dark-grown soybean seedlings[J].Plant physiol,1996,112(3):1357-1364.
[31] 张志良,李小方.植物生理学实验指导[M].北京:高等教育出版社,2009.
[32] YAN J Q,ZHU J,HE C X,et al.Quantitative trait loci analysis for the developmental behavior of tiller number in rice(Oryza sativa L.)[J].Theoretical and applied genetics,1998,97:267-274.
[33] WANG Y H,LI J Y.Branching in rice[J].Current opinion in plant biology,2011,14(1):94-99.
[34] 蒋彭炎.水稻分蘖的发生、控制与茎蘖成穗率的提高[J].中国稻米,1999(4):7-9.
[35] 黄耀祥,林青山.水稻超高产、特优质株型模式的构想和育种实践[J].广东农业科学,1994,94(4):1-6.
[36] 蒋彭炎,洪晓富.水稻三高一稳栽培法Ⅰ[J].种植世界,1996,96(9):10-12.
[37] XU H C,CAI T,WANG Z L,et al.Physiological basis for the differences of productive capacity among tillers in winter wheat[J].Journal of integrative agriculture,2015,14(10):1958-1970.
[38] CAI T,XU H C,PENG D L,et al.Exogenous hormonal application improves grain yield of wheat byoptimizing tiller productivity[J].Field crops research,2014,155:172-183.
[39] FRANK A B,BAUER A,BLACK A L.Carbohydrate,nitrogen and phosphorus concentrations of spring wheat leaves and stems[J].Agronomy journal,1989,81(3):524-528.
[40] WATERS M T,GUTJAHR C,BENNETT T,et al.Strigolactone signaling and evolution[J].Annual review plant biology,2017,68:291-322.
[41] ZOU J H,ZHANG S Y,ZHANG W P,et al.The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is requid for negative regulation of the outgrowth of axillary buds[J].Plant journal,2006,48(5):687-698.
[42] ZHANG S Y,LI G,FANG J,et al.The interactions among DWARF10,auxin and cytokinin underlie lateral bud outgrowth in rice[J].Journal of integrative plant biology,2010,52(7):626-638.