SSR purity identification of cucumber variety Jingyan Lvfeicui

doi:10.14088/j.cnki.issn0439-8114.2022.01.018

HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (1): 98-101.doi: 10.14088/j.cnki.issn0439-8114.2022.01.018

• Horticulture & Local Products • Previous Articles Next Articles

SSR purity identification of cucumber variety Jingyan Lvfeicui

MENG Shu-chun¹, XU Xiu-ping², LIU Li-gong¹, SONG Shun-hua¹, ZHANG Feng¹

1. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops （North China）/Key Laboratory of Urban Agriculture （North）, Ministry of Agriculture and Rural Affairs, Beijing 100097, China;
2. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2021-03-08 Online:2022-01-10 Published:2022-01-26

Abstract

Abstract: For ensuring the seed genetic quality, increasing the efficiency of application of the variety identification and seed genetic purity on cucumber hybrid seed, alternative 63 pairs of SSR primers have been applied in the screening the molecular markers on the cucumber Jingyan Lvfeicui and their parents. The results showed that 16 pairs of primers complementary to appear polymorphic bands between the parents, clear and stabilized, performing codominance, highly specificity, among hybrids, which can be used to distinct its parents and other varieties. The core SSR primers of Jingyan Lvfeicui were composed mainly of these 16 pairs of primers, formed the SSR fingerprint of Jingyan Lvfeicui, which provided technological information for seeds purity, authenticity of hybrid and parents purifying. Among them, the bands amplified by 10 pairs of primers were high distinguishable, the particularity was strong, as well as the characteristics of clear bands and the result agreed with field test.

Key words: Jingyan Lvfeicui cucumber, SSR, purity identification

CLC Number:

S642.2

MENG Shu-chun, XU Xiu-ping, LIU Li-gong, SONG Shun-hua, ZHANG Feng. SSR purity identification of cucumber variety Jingyan Lvfeicui[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(1): 98-101.

References

[1] 董玉琛,刘旭. 中国作物及其野生近缘植物(蔬菜作物卷下)[M]. 北京:中国农业出版社,2008.
[2] 王全,张桂华,苗伟利. 应用SSR 技术进行黄瓜杂交种种子纯度鉴定[J]. 长江蔬菜,2009(7):43-44.
[3] YASHITOLA J,THIRUMURUGAN T,SUNDARAM R M,et al.Assessment of purity of rice hybrid using microsatellite and STS markers[J]. Crop science,2002,42(4):1369-1373.
[4] ZHANG W W, PAN J S, HE H L, et al.Construction of a high density integrated genetic map for cucumber(Cucumis sativus L.)[J]. Theor Appl Genet, 2012,124: 249-259.
[5] LI Y H,YANG L M,DAWEI M P,et al.Fine genetic mapping of cp:A recessive gene for compact (dwarf) plant architecture in cucumber,Cucumis sativus L.[J]. Theor Appl Genet,2011,123: 973-983.
[6] ZHANG S P, MIAO H, GU X F, et al.A major quantitative trait locus conferring resistance to fusarium wilt was detected in cucumber by using recombinant inbred lines[J]. Mol Breeding,2014,34:1805-1815.
[7] HU J, WANG L, LI J.Comparison of genomic SSR and EST-SSR markers for estimating genetic diversity in cucumber[J]. Biologia plantarum, 2011, 55: 577-580.
[8] PANDEY S, ANSARI W A, MISHRA V K, et al.Genetic diversity in Indian cucumber based on microsatellite and morphological markers[J]. Biochemical systematics and ecology, 2013, 51:19-27.
[9] 张桂华,李家旺,张文珠,等. 利用SSR技术对黄瓜新品种津优35号进行种子纯度鉴定[J]. 北方园艺,2010(10):184-185.
[10] 崔兴华,韩毅科,杜胜利,等黄瓜新品种‘津优401’种子纯度的SSR鉴定[J]. 中国瓜菜,2015(3):38-39.
[11] 杨宏,刘小俊,梁根云,等. 黄瓜品种‘川绿2号’SSR指纹图谱的构建和纯度鉴定[J]. 西南农业学报,2016,29(2):374-378.
[12] 张桂华. 与黄瓜白粉病抗性相关基因紧密连锁的分子标记研究[D]. 陕西杨凌:西北农林科技大学,2003.
[13] 葛风伟,张海英,陈青君,等. 黄瓜SSR反应体系的建立[J]. 华北农学报,2004,19(2):5-9.
[14] 孟淑春,徐秀苹,宋顺华. 品种纯度检测助力蔬菜种业发展[J]. 种子,2020,39(4):161-164.
[15] 魏明丽. 浅谈种子纯度的检验方法[J]. 种子世界,2011(9):22.
[16] 陈越. 晋中市蔬菜作物种子质量分析研究[D]. 山西太谷:山西农业大学,2017.
[17] 王娇. “陕油803”油菜种子纯度鉴定及一个芥菜型油菜黄化基因的分子标记[D]. 陕西杨凌:西北农林科技大学,2014.

SSR purity identification of cucumber variety Jingyan Lvfeicui

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