Abstract: In order to speed up the molecular breeding process of strawberry resistance（Fragaria×ananassa Duch.）, the excellent hybrid offspring of strawberry were used as the test materials. At first,the resistance test was carried out, and the resistant genes of parents and different hybrid offsprings were screened, combined with SSR molecular marker technology. Finally, the resistant strains were screened out. The results showed that excellent offspring 13-53-100 and 13-52-407 had strong resistance to F. oxysporum, and excellent offspring 13-65-265 had the worst resistance to F. oxysporum. The screening result of resistance gene showed that, the parent Zijin Sijin and sweet Charlie had the resistance gene, the offspring 13-53-100 and 13-52-407 had a consistent band of parent sweet Charlie, which may have inherited the resistance of sweet Charlie.

Key words: strawberry（Fragaria×ananassa Duch.）, Fusarium oxysporum, resistance gene, SSR markers