Epidemiological investigation and species identification of Theileria in goats from Xiangxi Autonomous Prefecture

doi:10.14088/j.cnki.issn0439-8114.2022.13.023

Abstract

Abstract: The present study was conducted to investigate the infection status and genetic species of Theileria in goats from XiangXi Autonomous Prefecture of Hunan Province. From August 2018 to August 2019, PCR was used to detect the 18S rRNA gene of Theileria in 204 blood samples of goats from different regions of Xiangxi Autonomous Prefecture. The results showed that the positive rate of Theileria in goats was 81.86%, and the positive rates of that from black goats and local goats were 79.52% and 83.47%, respectively. The positive rates of Theileria in goats with house feeding and raised in free range were 75.78% and 92.10%, respectively. Also, the 18S rRNA sequences from 16 positive samples were randomly selected for sequencing and analysis. The results showed that the 18S rRNA gene sequences of these samples shared more than 99.50% genetic homology with Theileria luwenshuni strains prevalent in China, and all specimens collected in this study belonged to the same branch as that of T.luwenshuni strains. The results showed that Theileria was widely prevalent in goats in Xiangxi Autonomous Prefecture of Hunan Province, and T.luwenshuni was the dominant species among them. Therefore, the prevention and control of this disease should be strengthened.

Key words: Theileria luwenshuni, epidemiological investigation, molecular identification, goat, Xiangxi Autonomous Prefecture

CLC Number:

S852.72
S827

LI Hui, SONG Jin-qiu, TANG Guo-xiang, WU Yang-xiang, ZHANG Da-jun, WANG Xiao-ping, XIANG Xiao-han, WANG Xia, HE Lan, HOU Qiang-hong. Epidemiological investigation and species identification of Theileria in goats from Xiangxi Autonomous Prefecture[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(13): 125-127.

References

[1] 宋军科,彭先启,王雪婷,等. 羊吕氏泰勒虫陕西分离株的鉴定及遗传进化分析[J].中国兽医学报,2018, 38(2): 307-311.
[2] 陈倩,王坤轮,闫亚群,等. 中国不同地区羊吕氏泰勒虫分子流行病学调查及遗传进化分析[J]. 中国畜牧兽医,2020, 47(6): 1902-1909.
[3] 罗金,谭阳春,仇晓飞,等. 甘肃省庆阳地区泰勒虫病流行趋势及分子特征分析[J].中国兽医科学,2020,50(8):946-951.
[4] 齐萌,赵爱云,王海国,等. 新疆南疆绵羊泰勒虫病分子流行病学调查[J].中国病原生物学杂志,2017,12(11):1102-1105.
[5] 刘伯承. 湘西自治州山羊寄生虫病病原学调查[J].湖南畜牧兽医,2014(5):26-28.
[6] 朱玉涛,党娜娜,苏布赛,等. 新疆部分地区羊泰勒虫病的流行情况调查[J].中国预防兽医学报,2018,40(2): 164-166.
[7] 田万年,薛书江,贾立军,等. 吉林省部分地区羊泰勒虫病流行病学调查及分类鉴定[J].中国兽医杂志,2017,50(10): 13-16.
[8] 郝桂英,袁晓琴,陈永航,等. 四川省攀西地区山羊泰勒虫病血清学调查[J].中国动物检疫,2020,37(8): 21-25.
[9] BYARUHANGA C,AKURE P C,LUBEMBE D M, et al.Molecular detection and characterisation of protozoan and rickettsial pathogens in ticks from cattle in the pastoral area of Karamoja, Uganda[J]. Ticks Tick Borne Dis,2021, 12(4):101709.
[10] BENEDICTO B, CEYLAN O, MOUMOUNI P F A, et al. Molecular detection and assessment of risk factors for tick-borne diseases in sheep and goats from turkey[J]. Acta Parasitol,2020,65(3):723-732.
[11] 王坤轮,周永春,赵姗姗,等. 陕西麟游县山羊吕氏泰勒虫感染情况调查及遗传进化分析[J].动物医学进展,2020,41(4): 123-126.
[12] 孟林明,李有全,何海宁,等. 甘肃河西四地区羊泰勒虫病的流行病学调查[J]. 中国兽医科学,2012, 42(11): 1195-1199.
[13] 袁延庆,李有全,刘志杰,等. 我国南方地区羊泰勒虫病的流行病学调查[J].中国兽医科学,2013, 43(6): 551-556.