HUBEI AGRICULTURAL SCIENCES ›› 2023, Vol. 62 ›› Issue (8): 133-139.doi: 10.14088/j.cnki.issn0439-8114.2023.08.021

• Aquatic Sciences • Previous Articles     Next Articles

Comparison of anesthetic effect of MS-222 and eugenol on Xenocypris davidi

CHEN Jun-ping1, WU Hui-hui1, SHEN Fang-fang1, ZHANG Jia-xin1, YU Ruo-meng1, FU Yong-jie1, ZHAO Dao-quan1,2,3, XIE Guo-qiang2, SUN Wen-xi1   

  1. 1. Changyuan Branch, Henan Academy of Agricultural Sciences, Changyuan 453400,Henan,China;
    2. Henan Academy of Fishery Sciences, Zhengzhou 450044,China;
    3. Research Station for Field Scientific Observation of Aquatic Organisms in Yiluo River, Yellow River Basin, Lushi 472200,Henan,China
  • Received:2022-04-16 Online:2023-08-25 Published:2023-09-22

Abstract: In order to study the anesthesia effect of MS-222 and eugenol on Xenocypris davidi, the Xenocypris davidi with a body weight of (20.10±1.28) g was selected and the anesthesia effect of the Xenocypris davidi was observed under different conditions. The results showed that with the increase of the concentrations of the two anesthetics, the time spent in anesthesia for Xenocypris davidi showed a decreasing trend, the recovery time of the MS-222 group was shortened, and the recovery time of eugenol was prolonged. When the mass concentration of MS-222 was 120 mg/L, or the mass concentration of eugenol was 30~60 mg/L, the Xenocypris davidi could be anesthetized within 3 minutes and resuscitated within 5 minutes. With the increase of the concentration of the two anesthetics, the respiratory rate of the experimental fish first increased and then decreased. Under the condition of the same mass concentration of the two anesthetics, with the continuous increase of the water temperature, the anesthesia time of the Xenocypris davidi showed a decreasing trend. And the recovery time of the eugenol treatment group continued to shorten, while the recovery time after MS-222 anesthesia was not significantly different at 10~20 ℃, and was significantly prolonged at 25 ℃(P<0.05). After anesthesia, with the increase of exposure time in the air, the resuscitation time of the MS-222 group showed a continuously prolonged trend, and the resuscitation time of the eugenol group was shortened at 4 minutes, and then continued to prolong. The MS-222 of 120 mg/L and eugenol of 30~60 mg/L had good anesthesia effect on Xenocypris davidi, and both could be used as ideal anesthetics for Xenocypris davidi.

Key words: Xenocypris davidi, anesthesia time, recovery time, respiratory rate, MS-222, eugenol

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