Optimization of extraction process of total alkaloids from Pinellia ternata and their biological activity analysis

doi:10.14088/j.cnki.issn0439-8114.2024.05.027

Abstract

Abstract: Taking the tuber of Pinellia ternata as raw material and ethanol as ultrasonic-assisted extraction solvent, the influence of single factors such as ethanol volume fraction, ultrasonic time, material-liquid ratio and ultrasonic temperature on total alkaloids extraction rate was investigated. The orthogonal test was designed to optimize the extraction process, and the in vitro antioxidant and hypoglycemic activities of total alkaloids were tested. The results showed that the optimal extraction condition was ethanol volume fraction of 65%, extraction time of 60 min, the material-liquid ratio of 1∶45, ultrasonic temperature of 65 ℃, and the extraction rate of total alkaloids was 0.502%; the clearance of DPPH and ABTS radicals reached 89.90% and 43.60%, respectively, and the inhibition of α-glucosidase reached 86.40%. The process was simple and feasible, and the total alkaloids showed good in vitro antioxidant and hypoglycemic biological activities.

Key words: Pinellia ternata, total alkaloids, orthogonal test, antioxidant, hypoglycemic action

CLC Number:

R284.2

ZHAO Li-ping, ZHANG Wen-wen, CHEN Qiong, SUN Wei, ZHANG Wen-qian, MA Xiao-wen. Optimization of extraction process of total alkaloids from Pinellia ternata and their biological activity analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 151-155.

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