Screening, identification and culture conditions optimization of a highly efficient potassium-solubilizing bacteria

doi:10.14088/j.cnki.issn0439-8114.2024.04.006

Abstract

Abstract: Five strains of potassium-solubilizing bacteria were isolated and screened from black soil paddy field. One strain （S1）with high efficiency of potassium-solubilizing was screened by its morphological characteristics and potassium-solubilizing ability. The species of S1 was identified by 16S rDNA, and the culture conditions of the strain were optimized by the single factor test and orthogonal test. The results showed that the five strains of potassium-solubilizing bacteria were all able to decompose potassium feldspar effectively. Strain S1 had the highest potassium-solubilizing ability with a potassium-solubilizing rate of 57.03%, and the content of potassium-solubilizing in the fermentation broth was 3.852 mg/L, which was the best potassium-solubilizing bacteria. The potassium-solubilizing strain S1 was identified as Burkholderia ambifaria. The optimized culture conditions were carbon source 1.0% mannitol, nitrogen source 1.0% peptone, inorganic salt 0.5% K₂HPO₄, culture temperature 30 ℃, culture time 48 h, liquid volume 80 mL/250 mL, initial pH 6.5, and inoculation volume 5.0%.

Key words: lack soil, potassium-solubilizing bacteria, strain identification, culture conditions, orthogonal test

CLC Number:

S154.3

ZHANG Lei, QIU Lu-fan, LIU Li-hong, ZHAO Xue-yu, HAN Chun-mei. Screening, identification and culture conditions optimization of a highly efficient potassium-solubilizing bacteria[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(4): 30-36.

References

[1] TEOTIA P, KUMAR V, KUMAR M, et al.Probiotic microbiome: Potassium solubilization and plant productivity[J]. Probiotics in agroecosystem, 2017, 25:451-467.
[2] FRANS JM M.Physiological functions of mineral macronutrients[J]. Current opinion in plant biology, 2009, 12(3): 250-258.
[3] 吴翠云,蒋卉,李天红,等.土施钾肥对骏枣叶片光合特性及果实品质的影响[J].中国土壤与肥料, 2018(4):99-106.
[4] LEIGH R, WYN J.A hypothesis relating critical potassium concentrations for growth to the distribution and functions of this ion in the plant cell[J]. The new phytologist, 1984, 97(1): 1-13.
[5] MAATHUIS F, DALE S.Energization of potassium uptake in Arabidopsis thaliana[J]. Planta, 1993, 191(3): 302-307.
[6] CHRISTIAN Z,MEHMET S,EDGAR P.Potassium in agriculture-status and perspectives[J]. Journal of plant physiology,2014,171(9):656-669.
[7] WANG H Y, CHENG W, LI T, et al.Can nonexchangeable potassium be differentiated from structural potassium in soils?[J]. Pedosphere, 2016, 26(2): 206-215.
[8] 张梦旭,杨少峰,曾凡海,等.钾细菌的解钾机制及在烟草生产上的应用[J].福建农林大学学报(自然科学版),2017,46(4):373-378.
[9] LIU L, JIN Y, WANG J, et al.Comparation of spatial interpolation methods on slowly available potassium in soils[J]. IOP conference series: Earth and environmental science, 2019, 234(1): 012018.
[10] JING Y D, ZHU H F, DING H X, et al.Spatial variation in soil available potassium and temporal changes due to intrinsic and extrinsic factors: A 10-year study[J]. Journal of soil science and plant nutrition, 2022, 22(1): 1305-1314.
[11] 李新新,高新新,陈星,等.一株高效解钾菌的筛选、鉴定及发酵条件的优化[J].土壤学报,2014,51(2):381-388.
[12] FATHARANI R, RAHAYU Y S.Isolation and characterization of potassium-solubilizing bacteria from paddy rhizosphere (Oryza sativa L.)[J]. Journal of physics:Conference series,2018,1108(1): 012105.
[13] YALLAPPA M, SAVALAGI V P, SHRUTHI P.Effect of dual inoculation of potassium solubilizing bacteria and phosphorus solubilizing bacteria on nutrient content in maize crop[J]. Trends in biosciences, 2015, 8(16): 4402-4405.
[14] SUBHASHINI D V.Growth promotion and increased potassium uptake of tobacco by potassium-sobilizing bacterium Frateuria aurantia grown at different potassium levels in vertisols[J]. Communications in soil science and plant analysis,2014,46(2):210-220.
[15] 张成省,陈雪,张玉芹,等.烟草根际土壤中解钾细菌的分离与多样性分析[J].中国生态农业学报,2013,21(6):737-743.
[16] 陈易,程永毅,郭涛,等.一株具紫色土亲和性解钾菌的筛选及促生效应[J].西南大学学报(自然科学版),2016,38(5):58-65.
[17] 杜琪,赵新华,王华杰,等.低钾胁迫对玉米干物质和养分积累与分配的影响[J].沈阳农业大学学报,2017,48(3):257-264.
[18] 王建国,王德禄,王守宇,等.黑龙江农田养分平衡和养分水平的动态变化[J].农业系统科学与综合研究,2000(2):124-127.
[19] 张磊,都钧,孔丽丽,等.施磷对东北黑土区春玉米产量、磷素吸收利用及土壤磷素平衡的影响[J].东北农业科学,2020,45(5):38-42.
[20] 朱波,倪俊,高丽,等.土壤全磷全钾同时测定方法研究[J].安徽农业科学,2018,46(15):110-111.
[21] 索雲凯. 解钾菌的分离筛选及对水稻田土壤钾释放研究[D].黑龙江大庆:东北石油大学,2021.
[22] 刘紫嫣,马桥,由胜男,等.Burkholderia sp. IDO3中靛蓝合成基因的克隆表达及其合成特性[J].微生物学通报,2017,44(11):2634-2643.
[23] HAN H S, LEE K D.Phosphate and potassium solubilizing bacteria effect on mineral uptake, soil availability and growth of eggplant[J]. Research journal of agriculture and biological sciences, 2005, 1(2): 176-180.
[24] MAHENDRA V S R, SURENDER S, SATYA P T, et al. A modified plate assay for rapid screening of potassium-solubilizing bacteria[J].Pedosphere,2016,26(5):768-773.
[25] 燕红,于彩莲,包鑫,等.高效解磷兼解钾活性菌株分离筛选的初步研究[J].扬州大学学报(农业与生命科学版),2016,37(1):81-85,90.
[26] 许丽宁,徐敬棋,邵彩虹,等.再生稻根际促生菌的分离、筛选与鉴定[J].南方农业学报,2020,51(4):814-821.
[27] 于淼,吴红艳,冯健,等.解磷菌623-3的鉴定、培养条件优化及应用[J].四川农业大学学报,2020,38(5):572-579.
[28] SHENG X F, HE L Y.Solubilization of potassium-bearing minerals by a wild-type strain of Bacillus edaphicus and its mutants and increased potassium uptake by wheat[J]. Canadian journal of microbiology, 2006, 52(1): 66-72.
[29] BASAK B, SARKAR B, BISWAS D R.Bio-intervention of naturally occurring silicate minerals for alternative source of potassium:Challenges and opportunities[J]. Advances in agronomy, 2016, 141:115-145.
[30] JAVIER G,ALBERT G,JUAN A B.The nature of the carbon source rules the competition between PAO and denitrifiers in systems for simultaneous biological nitrogen and phosphorus removal[J]. Water research, 2011, 45(16): 4793-4802.
[31] 米前芬,向玉萍,王永敏,等.土生拉乌尔菌TGRB3的生物学特性及其不同氧气浓度条件下的汞甲基化[J].环境科学学报,2019,39(7):2240-2248.