Abstract: Pleurotus citrinopileatus was used as the test material, and the single factor test of the culture conditions was performed based on the best bag culture with sterilized raw materials. On the basis of the single factor experiment, the response surface optimization experiment was carried out with the mycelium growth rate as the response value by using the Box-Behnken central combination principle, and the optimization research was carried out on four different culture conditions in mycelium culture, including inoculum volume, pH, temperature and water content of culture material. The results showed that the influence of the four factors on the mycelium growth rate of Pleurotus citrinopileatus was temperature > inoculum volume > pH > water content of culture material. The optimal culture condition was inoculum volume of 7%, pH of 6.5, temperature of 26 ℃, and water content of culture material of 65%. Under this culture condition, the mycelium growth rate of Pleurotus citrinopileatus could reach 9.99 mm/d.

Key words: Pleurotus citrinopileatus, bag culture with sterilized raw materials, mycelium growth, culture conditions, response surface methodology