Study on extraction process of polysaccharide in Codonopsis pilosula based on response surface methodology

doi:10.14088/j.cnki.issn0439-8114.2022.12.024

Abstract

Abstract: The polysaccharide in Codonopsis pilosula was extracted by water extraction and alcohol precipitation. The effects of reflux time, extraction temperature and liquid-solid ratio on the extraction rate of the polysaccharide in Codonopsis pilosula were investigated by the single factor test. The extraction process was optimized by Box-Behnken response surface methodology. The optimal extraction process was liquid-solid ratio of 11.2∶1（mL/g）, extraction temperature of 80 ℃ and reflux time of 1.5 h. Under these conditions, the yield of Codonopsis pilosula polysaccharide was 19.38%, and the measured value was 0.573 percentage points lower than the theoretical value of the model, with a good fitting degree, which fully verified the accuracy of the model. The process optimization was reasonable and effective.

Key words: Codonopsis pilosula polysaccharide, extraction and separation, Box-Behnken design, response surface methodology

CLC Number:

TQ281

ZHANG Xue-ling, LIAN Sheng, QI Shu-lan, GUO Yi-yi, CHEN Jia-wei. Study on extraction process of polysaccharide in Codonopsis pilosula based on response surface methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(12): 120-123.

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