Optimization of ultrasonic-assisted salting-out assisted extraction of essential oil from Piper nigrum L. and its activity analysis

doi:10.14088/j.cnki.issn0439-8114.2024.05.029

Abstract

Abstract: The ultrasonic-assisted salting-out assisted steam distillation method was used to extract essential oil from Piper nigrum L.. Response surface methodology was used to determine the optimal extraction parameters such as liquid-solid ratio, ultrasonic time and NaCl concentration. The results showed that the optimal extraction condition of essential oil from Piper nigrum L. by ultrasonic-assisted distillation method was the liquid-solid ratio of 17∶1, ultrasonic time of 13 min, and NaCl concentration of 2.4%. Under this condition, the extraction yield of Piper nigrum L. essential oil was 1.79%. The extracts obtained by this method were tested for antioxidant and antibacterial activities. The results showed that Piper nigrum L. essential oil had a certain antioxidant capacity, and its scavenging ability on ABTS was much stronger than that on DPPH. The extract also showed strong growth inhibition against both Staphylococcus aureus and Pseudomonas solanacearum.

Key words: Piper nigrum L., essential oils, ultrasound, steam distillation, antioxidant, bacteriostatic

CLC Number:

TQ654.2

TAN Zi-yi, ZENG Jia-ru, LI Meng. Optimization of ultrasonic-assisted salting-out assisted extraction of essential oil from Piper nigrum L. and its activity analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 162-167.

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