Optimization of ultrasonic-assisted compound enzymatic hydrolysis process of corncob pretreated with organic acid

doi:10.14088/j.cnki.issn0439-8114.2024.05.030

Abstract

Abstract: The purpose was to destroy the tight structure of corncob lignocellulose for enzymatic hydrolysis and fermentation. The optimum organic acid and its concentration were determined by screening experiment firstly. Then the optimal composition and ratio of hydrolases for corncob were determined by compound enzymatic hydrolysis experiment. Finally, the optimal pretreatment process was optimized by response surface experiment. The results showed that the optimum process of enzymatic hydrolysis of corncob pretreated with 10% maleic acid was as follows： the liquid-solid ratio of 25∶1,ultrasonic power of 310 W, and ultrasonic treatment time of 34 min at 71 ℃, and the residue was hydrolyzed with cellulase, β-glucosidase and xylanase （1∶2∶1） for 48 h at pH 4.8 and temperature 50 ℃. Under this condition, the yield of reducing sugar was 34.49%,which was close to the cellulose content in corncob. The method was proved to be stable and reliable,and could provide preliminary technical support for ethanol production from corncob by fermentation.

Key words: ultrasonic-assisted, organic acid pretreatment, corncob, enzymatic hydrolysis, process optimization

CLC Number:

TS210.9

YU De-han, ZHU Ying-yi, LI Li, WU Hai-ling. Optimization of ultrasonic-assisted compound enzymatic hydrolysis process of corncob pretreated with organic acid[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 168-174.

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