Optimization of extraction process of total flavonoids from Pueraria montana and it’s antibacterial and antioxidant activities

doi:10.14088/j.cnki.issn0439-8114.2023.11.012

Abstract

Abstract: The antibacterial and antioxidant activities of total flavonoids from Pueraria montana （Loureiro） Merrill were studied. The water extraction technology of Pueraria montana was optimized by the single factor test and response surface method. The total flavonoids were characterized by ultraviolet spectrophotometry and high performance liquid chromatography. The biological activities of total flavonoids were evaluated by agar diffusion method, with DPPH·, ABTS⁺· and total reduction capacity as indicators. The results showed that the optimized extraction process of total flavonoids from Pueraria montana was as follows： Solid-liquid ratio （m∶V,g∶mL） of 1∶16, extraction time of 69 min, extraction times of 3 times, and the total flavonoids yield was 7.690%. Nine common flavonoids were detected in the optimized total flavonoids of Pueraria montana, the total content of which accounted for 91.81%. The total flavonoids of Pueraria montana had different antibacterial activities against 11 strains tested, and it was found to be moderately sensitive to Enterococcus faecalis and Bacillus thuringiensis for the first time, with MIC of 250, 500 mg/mL, respectively. It had strong scavenging ability and total reduction ability, and the IC₅₀ of DPPH· and ABTS⁺· free radicals were 0.398, 0.189 mg/mL, respectively.

Key words: Pueraria montana（Loureiro）Merrill, response surface, process optimization, total flavonoid, antibacterial, antioxidation

CLC Number:

R284.2

ZHENG Min, SU Fu-lian, YUAN Ming-yuan, LIU Yu, HUANG Dong-hai, ZHANG Wei, LI Yu, LIAO Lu-jing, LUO Kai, HE Mei-jun. Optimization of extraction process of total flavonoids from Pueraria montana and it’s antibacterial and antioxidant activities[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(11): 65-72.

References

[1] QI Q,ZHANG X Q,YAO L,et al.Pueratin improves diminished ovarian reserve by inhibiting apoptosis[J]. Experimental and therapeutic medicine,2021,22(6):1-10.
[2] NIU W,MIAO J,LI X,et al.Metabolomics combined with systematic pharmacology reveals the therapeutic effects of Salvia miltiorrhiza and Radix Pueraria lobata herb pair on type 2 diabetes rats[J].Journal of functional foods,2022,89(1):1423.
[3] 陈丽红,唐于平,王强.不同提取方法制备的葛根芩连配方颗粒抑菌药效研究[J].中成药,2012,34(10): 2016-2017.
[4] 庞会娜,范琳,肖凤琴,等.指纹图谱结合化学计量法对葛根抗氧化活性部位的药效物质筛选[J].中国药房,2021,32(7):839-844.
[5] 向志军,赵广荣,元英进,等.复方丹参的体外抗氧化活性研究[J].中草药,2006(2):211-213.
[6] 胡清文. 三种中药材的化学成分及其生物活性研究[D].济南:山东大学,2018.
[7] MIGUES V H,DAVID J M,GOMES A F,et al.Determination of soybean isoflavone by HPLC/DAD and simple UV spectroscopic analysis: A comparative study[J].Food analytical methods,2022, 15(2):367-376.
[8] 张晋,陈杨,曹飒丽,等.水煎煮法提取葛根总黄酮的动力学模型适用性研究[J].中国中药杂志,2013, 38(17):2788-2792.
[9] WOUMBO C Y,KUATE D,KLANG M J,et al.Valorization of glycine max (soybean) seed waste: Optimization of the microwave-assisted extraction (MAE) and characterization of polyphenols from soybean meal using response surface methodology (RSM)[J].Journal of chemistry,2021(11):1-12.
[10] BUCKOW R,KASTELL A,TEREFE N S,et al.Pressure and temperature effects on degradation kinetics and storage stability of total anthocyanins in blueberry juice[J].Journal of agricultural and food chemistry,2010, 58(18):10076-10084.
[11] 王萍,王宇鹤,辛爱洁,等.Box-Behnken响应面法优化粉葛配方颗粒提取工艺[J].中成药,2017,39(6): 1293-1296.
[12] KATHURIA D,DHIMAN A K,SUREKHA A.Effect of different extraction techniques and drying conditions for the assay of isoflavone from Harit soya seeds[J].Journal of food processing and preservation,2021,46(1):e16107.
[13] 袁名远,刘玉,黄东海,等.响应面优化复合藤茶固体饮料研究[J].食品科技,2021,46(8):96-103.
[14] 刘雨诗,刘娟汝,张存艳,等.微波萃取葛根总黄酮工艺及其抗氧化活性研究[J].时珍国医国药,2020,31(1): 68-72.
[15] SUN Y,YANG B,WU Y,et al. Structural characterization and antioxidant activities of κ-carrageenan oligosaccharides degraded by different methods[J].Food chemistry,2015(1),178:311-318.
[16] ZHAO T,LUO Y,ZHANG X,et al.Subcritical water extraction of bioactive compounds from Radix Puerariae and optimization study using response surface methodology[J].Chemical engineering communications,2019, 206(9):1-11.
[17] 杨青青,龚吉军.响应面法优化超声辅助葛根浸提工艺及浸提液抗氧化活性研究[J].食品安全质量检测学报,2021,12(13):5409-5417.
[18] 魏磊,王伟,侯玥莹,等.葛叶总黄酮的提取工艺优化及抗菌抗氧化能力研究[J].安徽农业科学,2021, 49(14):152-156,161.
[19] 范琳,王苗,马馨桐,等.葛根抗氧化活性有效部位的筛选研究[J].食品研究与开发,2021,42(6): 119-123.